Abundant variation in microsatellites of the parasitic nematode Trichostrongylus tenuis and linkage to a tandem repeat

P. C. D. Johnson, Lucy Webster, A. Adam, R. Buckland, D. A. Dawson, L. F. Keller

    Research output: Contribution to journalArticle

    42 Citations (Scopus)

    Abstract

    An understanding of how genes move between and within populations of parasitic nematodes is important in combating the evolution and spread of anthelmintic, resistance. Much has been learned by studying mitochondrial DNA markers, but autosomal markers such as microsatellites have been applied to only a few nematode species, despite their many advantages for studying gene flow in eukaryotes. Here, we describe the isolation of 307 microsatellites from Trichostrongylus tenuis, an intestinal nematode of red grouse. High levels of variation were revealed at sixteen microsatellite loci (including three sex-lined loci) in 111 male T tenuis nematodes collected from four hosts at a single grouse estate in Scotland (average H-e = 0.708; mean number of alleles = 12.2). A population genetic analysis detected no deviation from panmixia either between (F-ST = 0.00) or within hosts (F-IS = 0.015). We discuss the feasibility of developing microsatellites in parasitic nematodes and the problem of null alleles. We also describe a novel 146-bp repeat element, TteREP1, which is linked to two-thirds of the microsatellites sequenced and is associated with marker development failure. The sequence of TteREP1 is related to the TcREP-class of repeats found in several other trichostrongyloid species including Trichostrongylus colubriformis and Haemonchus contortus. (c) 2006 Elsevier B.V. All rights reserved.

    Original languageEnglish
    Pages (from-to)210-218
    Number of pages8
    JournalMolecular and Biochemical Parasitology
    Volume148
    Issue number2
    DOIs
    Publication statusPublished - Aug 2006

    Keywords

    • microsatellite
    • parasitic nematode
    • population genetics
    • repetitive elements
    • sex-linkage
    • Trichostronglylus tenuis
    • LAGOPUS-LAGOPUS-SCOTICUS
    • CAPTIVE RED GROUSE
    • HAEMONCHUS-CONTORTUS
    • EUKARYOTIC GENOMES
    • GENETIC-STRUCTURE
    • DRUG-RESISTANCE
    • POPULATIONS
    • DNA
    • LOCI
    • IDENTIFICATION

    Cite this

    Abundant variation in microsatellites of the parasitic nematode Trichostrongylus tenuis and linkage to a tandem repeat. / Johnson, P. C. D.; Webster, Lucy; Adam, A.; Buckland, R.; Dawson, D. A.; Keller, L. F.

    In: Molecular and Biochemical Parasitology, Vol. 148, No. 2, 08.2006, p. 210-218.

    Research output: Contribution to journalArticle

    Johnson, P. C. D. ; Webster, Lucy ; Adam, A. ; Buckland, R. ; Dawson, D. A. ; Keller, L. F. / Abundant variation in microsatellites of the parasitic nematode Trichostrongylus tenuis and linkage to a tandem repeat. In: Molecular and Biochemical Parasitology. 2006 ; Vol. 148, No. 2. pp. 210-218.
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    abstract = "An understanding of how genes move between and within populations of parasitic nematodes is important in combating the evolution and spread of anthelmintic, resistance. Much has been learned by studying mitochondrial DNA markers, but autosomal markers such as microsatellites have been applied to only a few nematode species, despite their many advantages for studying gene flow in eukaryotes. Here, we describe the isolation of 307 microsatellites from Trichostrongylus tenuis, an intestinal nematode of red grouse. High levels of variation were revealed at sixteen microsatellite loci (including three sex-lined loci) in 111 male T tenuis nematodes collected from four hosts at a single grouse estate in Scotland (average H-e = 0.708; mean number of alleles = 12.2). A population genetic analysis detected no deviation from panmixia either between (F-ST = 0.00) or within hosts (F-IS = 0.015). We discuss the feasibility of developing microsatellites in parasitic nematodes and the problem of null alleles. We also describe a novel 146-bp repeat element, TteREP1, which is linked to two-thirds of the microsatellites sequenced and is associated with marker development failure. The sequence of TteREP1 is related to the TcREP-class of repeats found in several other trichostrongyloid species including Trichostrongylus colubriformis and Haemonchus contortus. (c) 2006 Elsevier B.V. All rights reserved.",
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    AB - An understanding of how genes move between and within populations of parasitic nematodes is important in combating the evolution and spread of anthelmintic, resistance. Much has been learned by studying mitochondrial DNA markers, but autosomal markers such as microsatellites have been applied to only a few nematode species, despite their many advantages for studying gene flow in eukaryotes. Here, we describe the isolation of 307 microsatellites from Trichostrongylus tenuis, an intestinal nematode of red grouse. High levels of variation were revealed at sixteen microsatellite loci (including three sex-lined loci) in 111 male T tenuis nematodes collected from four hosts at a single grouse estate in Scotland (average H-e = 0.708; mean number of alleles = 12.2). A population genetic analysis detected no deviation from panmixia either between (F-ST = 0.00) or within hosts (F-IS = 0.015). We discuss the feasibility of developing microsatellites in parasitic nematodes and the problem of null alleles. We also describe a novel 146-bp repeat element, TteREP1, which is linked to two-thirds of the microsatellites sequenced and is associated with marker development failure. The sequence of TteREP1 is related to the TcREP-class of repeats found in several other trichostrongyloid species including Trichostrongylus colubriformis and Haemonchus contortus. (c) 2006 Elsevier B.V. All rights reserved.

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    KW - EUKARYOTIC GENOMES

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    KW - DRUG-RESISTANCE

    KW - POPULATIONS

    KW - DNA

    KW - LOCI

    KW - IDENTIFICATION

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    SP - 210

    EP - 218

    JO - Molecular and Biochemical Parasitology

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    SN - 0166-6851

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    ER -