Accumulation, Depuration, and Biological Effects of Polystyrene Microplastic Spheres and Adsorbed Cadmium and Benzo(a)pyrene on the Mussel Mytilus galloprovincialis

Rebecca von Hellfeld; Maria Zazuelo; Beñat Zaldibar; Miren Cajaraville; Amaia Orbea

doi:10.3390/toxics10010018

Accumulation, Depuration, and Biological Effects of Polystyrene Microplastic Spheres and Adsorbed Cadmium and Benzo(a)pyrene on the Mussel Mytilus galloprovincialis

Rebecca von Hellfeld, Maria Zazuelo, Beñat Zaldibar, Miren Cajaraville, Amaia Orbea^* (Corresponding Author)

^*Corresponding author for this work

University of the Basque Country

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

Filter feeders are target species for microplastic (MP) pollution, as particles can accumulate in the digestive system, disturbing feeding processes and becoming internalized in tissues. MPs may also carry pathogens or pollutants present in the environment. This work assessed the influence of polystyrene (PS) MP size and concentration on accumulation and depuration time and the role of MPs as vectors for metallic (Cd) and organic (benzo(a)pyrene, BaP) pollutants. One-day exposure to pristine MPs induced a concentration-dependent accumulation in the digestive gland (in the stomach and duct lumen), and after 3-day depuration, 45 µm MPs appeared between gill filaments, while 4.5 µm MPs also occurred within gill filaments. After 3-day exposure to contaminated 4.5 µm MPs, mussels showed increased BaP levels whilst Cd accumulation did not occur. Here, PS showed higher affinity to BaP than to Cd. Three-day exposure to pristine or contaminated MPs did not provoke significant alterations in antioxidant and peroxisomal enzyme activities in the gills and digestive gland nor in lysosomal membrane stability. Exposure to dissolved contaminants and to MP-BaP caused histological alterations in the digestive gland. In conclusion, these short-term studies suggest that MPs are ingested and internalized in a size-dependent manner and act as carriers of the persistent organic pollutant BaP

Original language	English
Article number	18
Number of pages	23
Journal	Toxics
Volume	10
Issue number	1
DOIs	https://doi.org/10.3390/toxics10010018
Publication status	Published - 5 Jan 2022

Bibliographical note

This work was performed in the framework of the European project “PLASTOX: Direct and indirect ecotoxicological impacts of microplastics on marine organisms” (JPI Oceans) and Centre for Advanced Studies (CAS) project “H2020 CAS6 Nanoplastics” funded by the European Commission- Joint Research Centre (JRC/A/05). It was funded by Spanish MINECO (NACE project CTM2016-81130-R), UPV/EHU (VRI grant PLASTOX) and the Basque Government through a grant to consolidated research groups (IT810-13 and IT302-19).

Data Availability Statement

Data are contained within the article.

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10.3390/toxics10010018Licence: CC BY

Von Hellfeld_etal_T_Accumulation_Depuration_and_VOR
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Marine Microplastic Pollution
Rebecca von Hellfeld (Examiner)
25 Nov 2022
Activity: Examination, Marking, Reviewing, Supervision, Teaching, or Tutoring › Teaching

Cite this

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title = "Accumulation, Depuration, and Biological Effects of Polystyrene Microplastic Spheres and Adsorbed Cadmium and Benzo(a)pyrene on the Mussel Mytilus galloprovincialis",

abstract = "Filter feeders are target species for microplastic (MP) pollution, as particles can accumulate in the digestive system, disturbing feeding processes and becoming internalized in tissues. MPs may also carry pathogens or pollutants present in the environment. This work assessed the influence of polystyrene (PS) MP size and concentration on accumulation and depuration time and the role of MPs as vectors for metallic (Cd) and organic (benzo(a)pyrene, BaP) pollutants. One-day exposure to pristine MPs induced a concentration-dependent accumulation in the digestive gland (in the stomach and duct lumen), and after 3-day depuration, 45 µm MPs appeared between gill filaments, while 4.5 µm MPs also occurred within gill filaments. After 3-day exposure to contaminated 4.5 µm MPs, mussels showed increased BaP levels whilst Cd accumulation did not occur. Here, PS showed higher affinity to BaP than to Cd. Three-day exposure to pristine or contaminated MPs did not provoke significant alterations in antioxidant and peroxisomal enzyme activities in the gills and digestive gland nor in lysosomal membrane stability. Exposure to dissolved contaminants and to MP-BaP caused histological alterations in the digestive gland. In conclusion, these short-term studies suggest that MPs are ingested and internalized in a size-dependent manner and act as carriers of the persistent organic pollutant BaP",

author = "{von Hellfeld}, Rebecca and Maria Zazuelo and Be{\~n}at Zaldibar and Miren Cajaraville and Amaia Orbea",

note = "This work was performed in the framework of the European project “PLASTOX: Direct and indirect ecotoxicological impacts of microplastics on marine organisms” (JPI Oceans) and Centre for Advanced Studies (CAS) project “H2020 CAS6 Nanoplastics” funded by the European Commission- Joint Research Centre (JRC/A/05). It was funded by Spanish MINECO (NACE project CTM2016-81130-R), UPV/EHU (VRI grant PLASTOX) and the Basque Government through a grant to consolidated research groups (IT810-13 and IT302-19).",

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AU - Zazuelo, Maria

AU - Zaldibar, Beñat

AU - Cajaraville, Miren

AU - Orbea, Amaia

N1 - This work was performed in the framework of the European project “PLASTOX: Direct and indirect ecotoxicological impacts of microplastics on marine organisms” (JPI Oceans) and Centre for Advanced Studies (CAS) project “H2020 CAS6 Nanoplastics” funded by the European Commission- Joint Research Centre (JRC/A/05). It was funded by Spanish MINECO (NACE project CTM2016-81130-R), UPV/EHU (VRI grant PLASTOX) and the Basque Government through a grant to consolidated research groups (IT810-13 and IT302-19).

PY - 2022/1/5

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N2 - Filter feeders are target species for microplastic (MP) pollution, as particles can accumulate in the digestive system, disturbing feeding processes and becoming internalized in tissues. MPs may also carry pathogens or pollutants present in the environment. This work assessed the influence of polystyrene (PS) MP size and concentration on accumulation and depuration time and the role of MPs as vectors for metallic (Cd) and organic (benzo(a)pyrene, BaP) pollutants. One-day exposure to pristine MPs induced a concentration-dependent accumulation in the digestive gland (in the stomach and duct lumen), and after 3-day depuration, 45 µm MPs appeared between gill filaments, while 4.5 µm MPs also occurred within gill filaments. After 3-day exposure to contaminated 4.5 µm MPs, mussels showed increased BaP levels whilst Cd accumulation did not occur. Here, PS showed higher affinity to BaP than to Cd. Three-day exposure to pristine or contaminated MPs did not provoke significant alterations in antioxidant and peroxisomal enzyme activities in the gills and digestive gland nor in lysosomal membrane stability. Exposure to dissolved contaminants and to MP-BaP caused histological alterations in the digestive gland. In conclusion, these short-term studies suggest that MPs are ingested and internalized in a size-dependent manner and act as carriers of the persistent organic pollutant BaP

AB - Filter feeders are target species for microplastic (MP) pollution, as particles can accumulate in the digestive system, disturbing feeding processes and becoming internalized in tissues. MPs may also carry pathogens or pollutants present in the environment. This work assessed the influence of polystyrene (PS) MP size and concentration on accumulation and depuration time and the role of MPs as vectors for metallic (Cd) and organic (benzo(a)pyrene, BaP) pollutants. One-day exposure to pristine MPs induced a concentration-dependent accumulation in the digestive gland (in the stomach and duct lumen), and after 3-day depuration, 45 µm MPs appeared between gill filaments, while 4.5 µm MPs also occurred within gill filaments. After 3-day exposure to contaminated 4.5 µm MPs, mussels showed increased BaP levels whilst Cd accumulation did not occur. Here, PS showed higher affinity to BaP than to Cd. Three-day exposure to pristine or contaminated MPs did not provoke significant alterations in antioxidant and peroxisomal enzyme activities in the gills and digestive gland nor in lysosomal membrane stability. Exposure to dissolved contaminants and to MP-BaP caused histological alterations in the digestive gland. In conclusion, these short-term studies suggest that MPs are ingested and internalized in a size-dependent manner and act as carriers of the persistent organic pollutant BaP

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DO - 10.3390/toxics10010018

M3 - Article

SN - 2305-6304

VL - 10

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M1 - 18

ER -

Accumulation, Depuration, and Biological Effects of Polystyrene Microplastic Spheres and Adsorbed Cadmium and Benzo(a)pyrene on the Mussel Mytilus galloprovincialis

Abstract

Bibliographical note

Data Availability Statement

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Activities

Marine Microplastic Pollution

Cite this