Accurate quantification of selenoproteins in human plasma/serum by isotope dilution ICP-MS: focus on selenoprotein P

M. Estela del Castillo Busto, Caroline Oster, Susana Cuello-Nunez, Christian L. Deitrich, Andrea Raab, Anna Konopka, Wolf D. Lehmann, Heidi Goenaga-Infante, Paola Fisicaro*

*Corresponding author for this work

Research output: Contribution to journalArticle

6 Citations (Scopus)
4 Downloads (Pure)

Abstract

A species-specific isotope dilution analysis (SS IDA) method was developed for the first time for the determination of selenoprotein P (SEPP1) in human plasma/serum at the protein level by double affinity HPLC-ICP-MS. In this regard, a standard and a spike of SEPP1 were produced by cell-free E. coli protein synthesis, where Se (ICP tag) was introduced in the form of selenomethionine (SeMet) allowing for the absolute SEPP1 quantification by ICP-MS. A complete characterization of the standard and the spike was carried out in terms of isotopic composition and Se mass fraction by collision-cell ICP-MS to ensure SI-traceability. Method development and validation were conducted using the reference materials BCR-637, extensively analysed for its Se species, and the SRM 1950, which provides reference values for Se species (including SEPP1). Stability of the isotope ratio R77/76 in the sample blends was tested for one month with negligible change. Relative expanded uncertainties of 5.7% and 7.7% were achieved for BCR-637 and SRM 1950 for mass fractions of 55.5 and 63.9 ng g(-1) Se for SEPP1, respectively. The developed SEPP1 SS IDA methodology could be a valuable tool to establish references values for selenoproteins in clinical chemistry and showed the potential of cell-free protein synthesis for the preparation of future stable isotope-labeled intact selenoproteins.

Original languageEnglish
Pages (from-to)1904-1912
Number of pages9
JournalJournal of Analytical Atomic Spectrometry
Volume31
Issue number9
Early online date26 Jul 2016
DOIs
Publication statusPublished - 1 Sep 2016

Keywords

  • HUMAN SERUM
  • SIMULTANEOUS SPECIATION
  • QUADRUPOLE MS
  • AFFINITY HPLC
  • SELENIUM
  • ONLINE

Cite this

Accurate quantification of selenoproteins in human plasma/serum by isotope dilution ICP-MS : focus on selenoprotein P. / Busto, M. Estela del Castillo; Oster, Caroline; Cuello-Nunez, Susana; Deitrich, Christian L.; Raab, Andrea; Konopka, Anna; Lehmann, Wolf D.; Goenaga-Infante, Heidi; Fisicaro, Paola.

In: Journal of Analytical Atomic Spectrometry, Vol. 31, No. 9, 01.09.2016, p. 1904-1912.

Research output: Contribution to journalArticle

Busto, MEDC, Oster, C, Cuello-Nunez, S, Deitrich, CL, Raab, A, Konopka, A, Lehmann, WD, Goenaga-Infante, H & Fisicaro, P 2016, 'Accurate quantification of selenoproteins in human plasma/serum by isotope dilution ICP-MS: focus on selenoprotein P', Journal of Analytical Atomic Spectrometry, vol. 31, no. 9, pp. 1904-1912. https://doi.org/10.1039/c6ja00122j
Busto, M. Estela del Castillo ; Oster, Caroline ; Cuello-Nunez, Susana ; Deitrich, Christian L. ; Raab, Andrea ; Konopka, Anna ; Lehmann, Wolf D. ; Goenaga-Infante, Heidi ; Fisicaro, Paola. / Accurate quantification of selenoproteins in human plasma/serum by isotope dilution ICP-MS : focus on selenoprotein P. In: Journal of Analytical Atomic Spectrometry. 2016 ; Vol. 31, No. 9. pp. 1904-1912.
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title = "Accurate quantification of selenoproteins in human plasma/serum by isotope dilution ICP-MS: focus on selenoprotein P",
abstract = "A species-specific isotope dilution analysis (SS IDA) method was developed for the first time for the determination of selenoprotein P (SEPP1) in human plasma/serum at the protein level by double affinity HPLC-ICP-MS. In this regard, a standard and a spike of SEPP1 were produced by cell-free E. coli protein synthesis, where Se (ICP tag) was introduced in the form of selenomethionine (SeMet) allowing for the absolute SEPP1 quantification by ICP-MS. A complete characterization of the standard and the spike was carried out in terms of isotopic composition and Se mass fraction by collision-cell ICP-MS to ensure SI-traceability. Method development and validation were conducted using the reference materials BCR-637, extensively analysed for its Se species, and the SRM 1950, which provides reference values for Se species (including SEPP1). Stability of the isotope ratio R77/76 in the sample blends was tested for one month with negligible change. Relative expanded uncertainties of 5.7{\%} and 7.7{\%} were achieved for BCR-637 and SRM 1950 for mass fractions of 55.5 and 63.9 ng g(-1) Se for SEPP1, respectively. The developed SEPP1 SS IDA methodology could be a valuable tool to establish references values for selenoproteins in clinical chemistry and showed the potential of cell-free protein synthesis for the preparation of future stable isotope-labeled intact selenoproteins.",
keywords = "HUMAN SERUM, SIMULTANEOUS SPECIATION, QUADRUPOLE MS, AFFINITY HPLC, SELENIUM, ONLINE",
author = "Busto, {M. Estela del Castillo} and Caroline Oster and Susana Cuello-Nunez and Deitrich, {Christian L.} and Andrea Raab and Anna Konopka and Lehmann, {Wolf D.} and Heidi Goenaga-Infante and Paola Fisicaro",
note = "Acknowledgements The research leading to these results was funded by the EMRP Joint Research Project “Metrology for metalloproteins” (HLT-05 2012). The EMRP is jointly funded by the EMRP participating countries within EURAMET and the European Union.",
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T2 - focus on selenoprotein P

AU - Busto, M. Estela del Castillo

AU - Oster, Caroline

AU - Cuello-Nunez, Susana

AU - Deitrich, Christian L.

AU - Raab, Andrea

AU - Konopka, Anna

AU - Lehmann, Wolf D.

AU - Goenaga-Infante, Heidi

AU - Fisicaro, Paola

N1 - Acknowledgements The research leading to these results was funded by the EMRP Joint Research Project “Metrology for metalloproteins” (HLT-05 2012). The EMRP is jointly funded by the EMRP participating countries within EURAMET and the European Union.

PY - 2016/9/1

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N2 - A species-specific isotope dilution analysis (SS IDA) method was developed for the first time for the determination of selenoprotein P (SEPP1) in human plasma/serum at the protein level by double affinity HPLC-ICP-MS. In this regard, a standard and a spike of SEPP1 were produced by cell-free E. coli protein synthesis, where Se (ICP tag) was introduced in the form of selenomethionine (SeMet) allowing for the absolute SEPP1 quantification by ICP-MS. A complete characterization of the standard and the spike was carried out in terms of isotopic composition and Se mass fraction by collision-cell ICP-MS to ensure SI-traceability. Method development and validation were conducted using the reference materials BCR-637, extensively analysed for its Se species, and the SRM 1950, which provides reference values for Se species (including SEPP1). Stability of the isotope ratio R77/76 in the sample blends was tested for one month with negligible change. Relative expanded uncertainties of 5.7% and 7.7% were achieved for BCR-637 and SRM 1950 for mass fractions of 55.5 and 63.9 ng g(-1) Se for SEPP1, respectively. The developed SEPP1 SS IDA methodology could be a valuable tool to establish references values for selenoproteins in clinical chemistry and showed the potential of cell-free protein synthesis for the preparation of future stable isotope-labeled intact selenoproteins.

AB - A species-specific isotope dilution analysis (SS IDA) method was developed for the first time for the determination of selenoprotein P (SEPP1) in human plasma/serum at the protein level by double affinity HPLC-ICP-MS. In this regard, a standard and a spike of SEPP1 were produced by cell-free E. coli protein synthesis, where Se (ICP tag) was introduced in the form of selenomethionine (SeMet) allowing for the absolute SEPP1 quantification by ICP-MS. A complete characterization of the standard and the spike was carried out in terms of isotopic composition and Se mass fraction by collision-cell ICP-MS to ensure SI-traceability. Method development and validation were conducted using the reference materials BCR-637, extensively analysed for its Se species, and the SRM 1950, which provides reference values for Se species (including SEPP1). Stability of the isotope ratio R77/76 in the sample blends was tested for one month with negligible change. Relative expanded uncertainties of 5.7% and 7.7% were achieved for BCR-637 and SRM 1950 for mass fractions of 55.5 and 63.9 ng g(-1) Se for SEPP1, respectively. The developed SEPP1 SS IDA methodology could be a valuable tool to establish references values for selenoproteins in clinical chemistry and showed the potential of cell-free protein synthesis for the preparation of future stable isotope-labeled intact selenoproteins.

KW - HUMAN SERUM

KW - SIMULTANEOUS SPECIATION

KW - QUADRUPOLE MS

KW - AFFINITY HPLC

KW - SELENIUM

KW - ONLINE

U2 - 10.1039/c6ja00122j

DO - 10.1039/c6ja00122j

M3 - Article

VL - 31

SP - 1904

EP - 1912

JO - Journal of Analytical Atomic Spectrometry

JF - Journal of Analytical Atomic Spectrometry

SN - 0267-9477

IS - 9

ER -