Acetate utilization and butyryl coenzyme A (CoA)

acetate-CoA transferase in butyrate-producing bacteria from the human large intestine

Sylvia H Duncan, Adela Barcenilla, Colin S Stewart, Susan E Pryde, Harry J Flint

Research output: Contribution to journalArticle

203 Citations (Scopus)

Abstract

Seven strains of Roseburia sp., Faecalibacterium prausnitzii, and Coprococcus sp. from the human gut that produce high levels of butyric acid in vitro were studied with respect to key butyrate pathway enzymes and fermentation patterns. Strains of Roseburia sp. and F. prausnitzii possessed butyryl coenzyme A (CoA):acetate-CoA transferase and acetate kinase activities, but butyrate kinase activity was not detectable either in growing or in stationary-phase cultures. Although unable to use acetate as a sole source of energy, these strains showed net utilization of acetate during growth on glucose. In contrast, Coprococcus sp. strain L2-50 is a net producer of acetate and possessed detectable butyrate kinase, acetate kinase, and butyryl-CoA:acetate-CoA transferase activities. These results demonstrate that different functionally distinct groups of butyrate-producing bacteria are present in the human large intestine.

Original languageEnglish
Pages (from-to)5186-5190
Number of pages5
JournalApplied and Environmental Microbiology
Volume68
Issue number10
DOIs
Publication statusPublished - Oct 2002

Keywords

  • germinated barley foodstuff
  • 16s ribosomal-RNA
  • colonic mucosa
  • human feces
  • human gut
  • butyrivibrio
  • fermentation
  • pathways
  • colitis
  • growth

Cite this

Acetate utilization and butyryl coenzyme A (CoA) : acetate-CoA transferase in butyrate-producing bacteria from the human large intestine. / Duncan, Sylvia H; Barcenilla, Adela; Stewart, Colin S; Pryde, Susan E; Flint, Harry J.

In: Applied and Environmental Microbiology, Vol. 68, No. 10, 10.2002, p. 5186-5190.

Research output: Contribution to journalArticle

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abstract = "Seven strains of Roseburia sp., Faecalibacterium prausnitzii, and Coprococcus sp. from the human gut that produce high levels of butyric acid in vitro were studied with respect to key butyrate pathway enzymes and fermentation patterns. Strains of Roseburia sp. and F. prausnitzii possessed butyryl coenzyme A (CoA):acetate-CoA transferase and acetate kinase activities, but butyrate kinase activity was not detectable either in growing or in stationary-phase cultures. Although unable to use acetate as a sole source of energy, these strains showed net utilization of acetate during growth on glucose. In contrast, Coprococcus sp. strain L2-50 is a net producer of acetate and possessed detectable butyrate kinase, acetate kinase, and butyryl-CoA:acetate-CoA transferase activities. These results demonstrate that different functionally distinct groups of butyrate-producing bacteria are present in the human large intestine.",
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AU - Flint, Harry J

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AB - Seven strains of Roseburia sp., Faecalibacterium prausnitzii, and Coprococcus sp. from the human gut that produce high levels of butyric acid in vitro were studied with respect to key butyrate pathway enzymes and fermentation patterns. Strains of Roseburia sp. and F. prausnitzii possessed butyryl coenzyme A (CoA):acetate-CoA transferase and acetate kinase activities, but butyrate kinase activity was not detectable either in growing or in stationary-phase cultures. Although unable to use acetate as a sole source of energy, these strains showed net utilization of acetate during growth on glucose. In contrast, Coprococcus sp. strain L2-50 is a net producer of acetate and possessed detectable butyrate kinase, acetate kinase, and butyryl-CoA:acetate-CoA transferase activities. These results demonstrate that different functionally distinct groups of butyrate-producing bacteria are present in the human large intestine.

KW - germinated barley foodstuff

KW - 16s ribosomal-RNA

KW - colonic mucosa

KW - human feces

KW - human gut

KW - butyrivibrio

KW - fermentation

KW - pathways

KW - colitis

KW - growth

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JF - Applied and Environmental Microbiology

SN - 0099-2240

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