Activation mechanism dependent surface exposure of cellular factor XIII on activated platelets and platelet microparticles

Laura  Somodi; Ildikó Beke  Debreceni; Gréta  Kis; Marco  Cozzolino; János  Kappelmayer; Miklós  Antal; György  Panyi; Helga  Bárdos; Nicola Mutch; László  Muszbek

doi:10.1111/jth.15668

Activation mechanism dependent surface exposure of cellular factor XIII on activated platelets and platelet microparticles

Laura Somodi, Ildikó Beke Debreceni, Gréta Kis, Marco Cozzolino, János Kappelmayer, Miklós Antal, György Panyi, Helga Bárdos, Nicola Mutch, László Muszbek^* (Corresponding Author)

^*Corresponding author for this work

University of Debrecen

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

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Abstract

Background: Platelets contain a high amount of potentially active A subunit dimer of coagulation factor XIII (cellular FXIII; cFXIII). It is of cytoplasmic localization, not secreted, but becomes translocated to the surface of platelets activated by convulxin and thrombin (CVX+Thr).
Objective: To explore the difference in cFXIII translocation between receptor mediated and non-receptor mediated platelet activation and if translocation can also be detected on platelet-derived microparticles. Our aim was also to shed some light on the mechanism of cFXIII translocation.
Methods: Gel-filtered platelets were activated by CVX+Thr or Ca2+-ionophore (calcimycin). The translocation of cFXIII and phosphatidylserine (PS) to the surface of activated platelets and platelet-derived microparticles was investigated by flow cytometry, immunofluorescence and immune electron microscopy. Fluo-4-AM fluorescence was used for the measurement of intracellular Ca2+ concentration.
Results: Receptor mediated activation by CVX+Thr exposed cFXIII to the surface of over 60% of platelets. Electron microscopy revealed microparticles with preserved membrane structure and microparticles devoid of labeling for membrane glycoprotein CD41a. cFXIII was observed on both types of microparticles but was more abundant in the absence of CD41a. Rhosin, a RhoA inhibitor significantly decreased cFXIII translocation. Non-receptor mediated activation of platelets by calcimycin elevated intracellular Ca2+ concentration, induced the translocation of PS to the surface of platelets and microparticles, but failed to expose cFXIII.
Conclusions: The elevation of intracellular Ca2+ concentration is sufficient for the translocation of PS from the internal layer of the membrane, while the translocation of cFXIII from the platelet cytoplasm requires additional receptor mediated mechanism(s).

Original language	English
Pages (from-to)	1223–1235
Number of pages	13
Journal	Journal of Thrombosis and Haemostasis
Volume	20
Issue number	5
Early online date	21 Feb 2022
DOIs	https://doi.org/10.1111/jth.15668
Publication status	Published - 20 Apr 2022

Bibliographical note

ACKNOWLEDGEMENTS
The authors are indebted to Prof. Edit Buzás MD, PhD (Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary) for the expert discussion on extracellular vesicles. We also thank Ms. Ágnes Bana (Faculty of Medicine, University of Debrecen, Debrecen, Hungary) for the expert assistance in immunofluorescence studies and to Ms. Fanni Puter (Faculty of Medicine, University of Debrecen, Debrecen, Hungary) for her technical help.
The research was funded by a grant from the National Research, Development and Innovation Office (NKFIH) (K129287), by the GINOP 2.3.2-15-2016-00050 project co-financed by the European Union and the European Regional Development Fund and by the Hungarian Academy of Science (11014 project). M.C. is a Marie Skłodowska-Curie Innovative Training Network (ITN) fellow (grant Agreement number: 813834 - pHioniC - H2020-MSCA-ITN-2018).
N.J.M was funded by the British Heart Foundation PG/15/82/31721, Friends of Anchor and the Aberdeen Development Trust.

Data Availability Statement

SUPPORTING INFORMATION
Additional supporting information may be found in the online version of the article at the publisher’s website.

Keywords

cell-derived microparticles
factor XIII
flow cytometry
immune electron microscopy
platelet activation

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Cite this

@article{19849d297652402eab934da692bf3c1d,

title = "Activation mechanism dependent surface exposure of cellular factor XIII on activated platelets and platelet microparticles",

abstract = "Background: Platelets contain a high amount of potentially active A subunit dimer of coagulation factor XIII (cellular FXIII; cFXIII). It is of cytoplasmic localization, not secreted, but becomes translocated to the surface of platelets activated by convulxin and thrombin (CVX+Thr).Objective: To explore the difference in cFXIII translocation between receptor mediated and non-receptor mediated platelet activation and if translocation can also be detected on platelet-derived microparticles. Our aim was also to shed some light on the mechanism of cFXIII translocation.Methods: Gel-filtered platelets were activated by CVX+Thr or Ca2+-ionophore (calcimycin). The translocation of cFXIII and phosphatidylserine (PS) to the surface of activated platelets and platelet-derived microparticles was investigated by flow cytometry, immunofluorescence and immune electron microscopy. Fluo-4-AM fluorescence was used for the measurement of intracellular Ca2+ concentration.Results: Receptor mediated activation by CVX+Thr exposed cFXIII to the surface of over 60% of platelets. Electron microscopy revealed microparticles with preserved membrane structure and microparticles devoid of labeling for membrane glycoprotein CD41a. cFXIII was observed on both types of microparticles but was more abundant in the absence of CD41a. Rhosin, a RhoA inhibitor significantly decreased cFXIII translocation. Non-receptor mediated activation of platelets by calcimycin elevated intracellular Ca2+ concentration, induced the translocation of PS to the surface of platelets and microparticles, but failed to expose cFXIII.Conclusions: The elevation of intracellular Ca2+ concentration is sufficient for the translocation of PS from the internal layer of the membrane, while the translocation of cFXIII from the platelet cytoplasm requires additional receptor mediated mechanism(s).",

keywords = "cell-derived microparticles, factor XIII, flow cytometry, immune electron microscopy, platelet activation",

author = "Laura Somodi and Debreceni, {Ildik{\'o} Beke} and Gr{\'e}ta Kis and Marco Cozzolino and J{\'a}nos Kappelmayer and Mikl{\'o}s Antal and Gy{\"o}rgy Panyi and Helga B{\'a}rdos and Nicola Mutch and L{\'a}szl{\'o} Muszbek",

note = "ACKNOWLEDGEMENTS The authors are indebted to Prof. Edit Buz{\'a}s MD, PhD (Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary) for the expert discussion on extracellular vesicles. We also thank Ms. {\'A}gnes Bana (Faculty of Medicine, University of Debrecen, Debrecen, Hungary) for the expert assistance in immunofluorescence studies and to Ms. Fanni Puter (Faculty of Medicine, University of Debrecen, Debrecen, Hungary) for her technical help. The research was funded by a grant from the National Research, Development and Innovation Office (NKFIH) (K129287), by the GINOP 2.3.2-15-2016-00050 project co-financed by the European Union and the European Regional Development Fund and by the Hungarian Academy of Science (11014 project). M.C. is a Marie Sk{\l}odowska-Curie Innovative Training Network (ITN) fellow (grant Agreement number: 813834 - pHioniC - H2020-MSCA-ITN-2018). N.J.M was funded by the British Heart Foundation PG/15/82/31721, Friends of Anchor and the Aberdeen Development Trust.",

year = "2022",

month = apr,

day = "20",

doi = "10.1111/jth.15668",

language = "English",

volume = "20",

pages = "1223–1235",

journal = "Journal of Thrombosis and Haemostasis",

issn = "1538-7933",

publisher = "Wiley-Blackwell ",

number = "5",

}

TY - JOUR

T1 - Activation mechanism dependent surface exposure of cellular factor XIII on activated platelets and platelet microparticles

AU - Somodi, Laura

AU - Debreceni, Ildikó Beke

AU - Kis, Gréta

AU - Cozzolino, Marco

AU - Kappelmayer, János

AU - Antal, Miklós

AU - Panyi, György

AU - Bárdos, Helga

AU - Mutch, Nicola

AU - Muszbek, László

N1 - ACKNOWLEDGEMENTS The authors are indebted to Prof. Edit Buzás MD, PhD (Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary) for the expert discussion on extracellular vesicles. We also thank Ms. Ágnes Bana (Faculty of Medicine, University of Debrecen, Debrecen, Hungary) for the expert assistance in immunofluorescence studies and to Ms. Fanni Puter (Faculty of Medicine, University of Debrecen, Debrecen, Hungary) for her technical help. The research was funded by a grant from the National Research, Development and Innovation Office (NKFIH) (K129287), by the GINOP 2.3.2-15-2016-00050 project co-financed by the European Union and the European Regional Development Fund and by the Hungarian Academy of Science (11014 project). M.C. is a Marie Skłodowska-Curie Innovative Training Network (ITN) fellow (grant Agreement number: 813834 - pHioniC - H2020-MSCA-ITN-2018). N.J.M was funded by the British Heart Foundation PG/15/82/31721, Friends of Anchor and the Aberdeen Development Trust.

PY - 2022/4/20

Y1 - 2022/4/20

N2 - Background: Platelets contain a high amount of potentially active A subunit dimer of coagulation factor XIII (cellular FXIII; cFXIII). It is of cytoplasmic localization, not secreted, but becomes translocated to the surface of platelets activated by convulxin and thrombin (CVX+Thr).Objective: To explore the difference in cFXIII translocation between receptor mediated and non-receptor mediated platelet activation and if translocation can also be detected on platelet-derived microparticles. Our aim was also to shed some light on the mechanism of cFXIII translocation.Methods: Gel-filtered platelets were activated by CVX+Thr or Ca2+-ionophore (calcimycin). The translocation of cFXIII and phosphatidylserine (PS) to the surface of activated platelets and platelet-derived microparticles was investigated by flow cytometry, immunofluorescence and immune electron microscopy. Fluo-4-AM fluorescence was used for the measurement of intracellular Ca2+ concentration.Results: Receptor mediated activation by CVX+Thr exposed cFXIII to the surface of over 60% of platelets. Electron microscopy revealed microparticles with preserved membrane structure and microparticles devoid of labeling for membrane glycoprotein CD41a. cFXIII was observed on both types of microparticles but was more abundant in the absence of CD41a. Rhosin, a RhoA inhibitor significantly decreased cFXIII translocation. Non-receptor mediated activation of platelets by calcimycin elevated intracellular Ca2+ concentration, induced the translocation of PS to the surface of platelets and microparticles, but failed to expose cFXIII.Conclusions: The elevation of intracellular Ca2+ concentration is sufficient for the translocation of PS from the internal layer of the membrane, while the translocation of cFXIII from the platelet cytoplasm requires additional receptor mediated mechanism(s).

AB - Background: Platelets contain a high amount of potentially active A subunit dimer of coagulation factor XIII (cellular FXIII; cFXIII). It is of cytoplasmic localization, not secreted, but becomes translocated to the surface of platelets activated by convulxin and thrombin (CVX+Thr).Objective: To explore the difference in cFXIII translocation between receptor mediated and non-receptor mediated platelet activation and if translocation can also be detected on platelet-derived microparticles. Our aim was also to shed some light on the mechanism of cFXIII translocation.Methods: Gel-filtered platelets were activated by CVX+Thr or Ca2+-ionophore (calcimycin). The translocation of cFXIII and phosphatidylserine (PS) to the surface of activated platelets and platelet-derived microparticles was investigated by flow cytometry, immunofluorescence and immune electron microscopy. Fluo-4-AM fluorescence was used for the measurement of intracellular Ca2+ concentration.Results: Receptor mediated activation by CVX+Thr exposed cFXIII to the surface of over 60% of platelets. Electron microscopy revealed microparticles with preserved membrane structure and microparticles devoid of labeling for membrane glycoprotein CD41a. cFXIII was observed on both types of microparticles but was more abundant in the absence of CD41a. Rhosin, a RhoA inhibitor significantly decreased cFXIII translocation. Non-receptor mediated activation of platelets by calcimycin elevated intracellular Ca2+ concentration, induced the translocation of PS to the surface of platelets and microparticles, but failed to expose cFXIII.Conclusions: The elevation of intracellular Ca2+ concentration is sufficient for the translocation of PS from the internal layer of the membrane, while the translocation of cFXIII from the platelet cytoplasm requires additional receptor mediated mechanism(s).

KW - cell-derived microparticles

KW - factor XIII

KW - flow cytometry

KW - immune electron microscopy

KW - platelet activation

U2 - 10.1111/jth.15668

DO - 10.1111/jth.15668

M3 - Article

SN - 1538-7933

VL - 20

SP - 1223

EP - 1235

JO - Journal of Thrombosis and Haemostasis

JF - Journal of Thrombosis and Haemostasis

IS - 5

ER -

Activation mechanism dependent surface exposure of cellular factor XIII on activated platelets and platelet microparticles

Abstract

Bibliographical note

Data Availability Statement

Keywords

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