Abstract
1. The short-term effect of oral feeding on albumin synthesis rate was investigated in 12 healthy volunteers using two meal regimens. Albumin synthesis was measured over 90 min after injection of a 'flooding' amount (43 mg/kg body weight) of phenylalanine enriched to 7.5, 10 or 15 atoms % with the stable isotope [ring-2H5]phenylalanine. 2. In one set of subjects, consumption of five small hourly meals resulted in a consistent and significant increase (P <0.05) in albumin fractional synthesis rate from a mean (+/- SEM) fasting value of 5.8 (+/- 0.4)%/day to 7.1 (+/- 0.4)%/day in the fed state. 3. A second study in which albumin synthesis was measured 30 min after consumption of a single larger meal was carried out in another set of volunteers. The fractional rate of albumin synthesis was again significantly elevated after feeding (P <0.05), rising from 7.1 (+/- 0.4)%/day in the fasted state to 9.1 (+/- 0.6)%/day in the fed state. In both studies, similar responses were observed in the absolute rate of albumin synthesis (mg day-1 kg-1). 4. Albumin secretion time was significantly shorter (P <0.05) after feeding in both studies, suggesting that the acute stimulation in albumin synthesis observed after feeding may in part be mediated via a post-transcriptional mechanism. 5. The response of total liver protein synthesis to oral feeding was investigated in an animal model employing adult rats studied with a flooding amount of [2,6-3H]phenylalanine.(ABSTRACT TRUNCATED AT 250 WORDS)
Original language | English |
---|---|
Pages (from-to) | 235-42 |
Number of pages | 8 |
Journal | Clinical Science |
Volume | 88 |
Issue number | 2 |
Publication status | Published - 1 Feb 1995 |
Keywords
- Adult
- Animals
- Eating
- Electrophoresis, Polyacrylamide Gel
- Female
- Humans
- Liver
- Male
- Phenylalanine
- Protein Biosynthesis
- Rats
- Rats, Wistar
- Serum Albumin
- exploration
- animal
- human
- comparative study
- radionuclide study
- albumin
- feeding
- oral administration
- phenylalanine
- isotope labelling
- rat
- protein synthesis
- rodentia
- mammalia
- vertebrata