Acute stimulation of albumin synthesis rate with oral meal feeding in healthy subjects measured with [ring-2H5]phenylalanine

K A Hunter, P E Ballmer, Susan Elizabeth Anderson, J Broom, P J Garlick, M A McNurlan

Research output: Contribution to journalArticle

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Abstract

1. The short-term effect of oral feeding on albumin synthesis rate was investigated in 12 healthy volunteers using two meal regimens. Albumin synthesis was measured over 90 min after injection of a 'flooding' amount (43 mg/kg body weight) of phenylalanine enriched to 7.5, 10 or 15 atoms % with the stable isotope [ring-2H5]phenylalanine. 2. In one set of subjects, consumption of five small hourly meals resulted in a consistent and significant increase (P <0.05) in albumin fractional synthesis rate from a mean (+/- SEM) fasting value of 5.8 (+/- 0.4)%/day to 7.1 (+/- 0.4)%/day in the fed state. 3. A second study in which albumin synthesis was measured 30 min after consumption of a single larger meal was carried out in another set of volunteers. The fractional rate of albumin synthesis was again significantly elevated after feeding (P <0.05), rising from 7.1 (+/- 0.4)%/day in the fasted state to 9.1 (+/- 0.6)%/day in the fed state. In both studies, similar responses were observed in the absolute rate of albumin synthesis (mg day-1 kg-1). 4. Albumin secretion time was significantly shorter (P <0.05) after feeding in both studies, suggesting that the acute stimulation in albumin synthesis observed after feeding may in part be mediated via a post-transcriptional mechanism. 5. The response of total liver protein synthesis to oral feeding was investigated in an animal model employing adult rats studied with a flooding amount of [2,6-3H]phenylalanine.(ABSTRACT TRUNCATED AT 250 WORDS)
Original languageEnglish
Pages (from-to)235-42
Number of pages8
JournalClinical Science
Volume88
Issue number2
Publication statusPublished - 1 Feb 1995

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Phenylalanine
Meals
Albumins
Healthy Volunteers
Isotopes
Volunteers
Fasting
Animal Models
Body Weight
Injections
Liver
Proteins

Keywords

  • Adult
  • Animals
  • Eating
  • Electrophoresis, Polyacrylamide Gel
  • Female
  • Humans
  • Liver
  • Male
  • Phenylalanine
  • Protein Biosynthesis
  • Rats
  • Rats, Wistar
  • Serum Albumin
  • exploration
  • animal
  • human
  • comparative study
  • radionuclide study
  • albumin
  • feeding
  • oral administration
  • phenylalanine
  • isotope labelling
  • rat
  • protein synthesis
  • rodentia
  • mammalia
  • vertebrata

Cite this

Hunter, K. A., Ballmer, P. E., Anderson, S. E., Broom, J., Garlick, P. J., & McNurlan, M. A. (1995). Acute stimulation of albumin synthesis rate with oral meal feeding in healthy subjects measured with [ring-2H5]phenylalanine. Clinical Science, 88(2), 235-42.

Acute stimulation of albumin synthesis rate with oral meal feeding in healthy subjects measured with [ring-2H5]phenylalanine. / Hunter, K A; Ballmer, P E; Anderson, Susan Elizabeth; Broom, J; Garlick, P J; McNurlan, M A.

In: Clinical Science, Vol. 88, No. 2, 01.02.1995, p. 235-42.

Research output: Contribution to journalArticle

Hunter, KA, Ballmer, PE, Anderson, SE, Broom, J, Garlick, PJ & McNurlan, MA 1995, 'Acute stimulation of albumin synthesis rate with oral meal feeding in healthy subjects measured with [ring-2H5]phenylalanine', Clinical Science, vol. 88, no. 2, pp. 235-42.
Hunter KA, Ballmer PE, Anderson SE, Broom J, Garlick PJ, McNurlan MA. Acute stimulation of albumin synthesis rate with oral meal feeding in healthy subjects measured with [ring-2H5]phenylalanine. Clinical Science. 1995 Feb 1;88(2):235-42.
Hunter, K A ; Ballmer, P E ; Anderson, Susan Elizabeth ; Broom, J ; Garlick, P J ; McNurlan, M A. / Acute stimulation of albumin synthesis rate with oral meal feeding in healthy subjects measured with [ring-2H5]phenylalanine. In: Clinical Science. 1995 ; Vol. 88, No. 2. pp. 235-42.
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N2 - 1. The short-term effect of oral feeding on albumin synthesis rate was investigated in 12 healthy volunteers using two meal regimens. Albumin synthesis was measured over 90 min after injection of a 'flooding' amount (43 mg/kg body weight) of phenylalanine enriched to 7.5, 10 or 15 atoms % with the stable isotope [ring-2H5]phenylalanine. 2. In one set of subjects, consumption of five small hourly meals resulted in a consistent and significant increase (P <0.05) in albumin fractional synthesis rate from a mean (+/- SEM) fasting value of 5.8 (+/- 0.4)%/day to 7.1 (+/- 0.4)%/day in the fed state. 3. A second study in which albumin synthesis was measured 30 min after consumption of a single larger meal was carried out in another set of volunteers. The fractional rate of albumin synthesis was again significantly elevated after feeding (P <0.05), rising from 7.1 (+/- 0.4)%/day in the fasted state to 9.1 (+/- 0.6)%/day in the fed state. In both studies, similar responses were observed in the absolute rate of albumin synthesis (mg day-1 kg-1). 4. Albumin secretion time was significantly shorter (P <0.05) after feeding in both studies, suggesting that the acute stimulation in albumin synthesis observed after feeding may in part be mediated via a post-transcriptional mechanism. 5. The response of total liver protein synthesis to oral feeding was investigated in an animal model employing adult rats studied with a flooding amount of [2,6-3H]phenylalanine.(ABSTRACT TRUNCATED AT 250 WORDS)

AB - 1. The short-term effect of oral feeding on albumin synthesis rate was investigated in 12 healthy volunteers using two meal regimens. Albumin synthesis was measured over 90 min after injection of a 'flooding' amount (43 mg/kg body weight) of phenylalanine enriched to 7.5, 10 or 15 atoms % with the stable isotope [ring-2H5]phenylalanine. 2. In one set of subjects, consumption of five small hourly meals resulted in a consistent and significant increase (P <0.05) in albumin fractional synthesis rate from a mean (+/- SEM) fasting value of 5.8 (+/- 0.4)%/day to 7.1 (+/- 0.4)%/day in the fed state. 3. A second study in which albumin synthesis was measured 30 min after consumption of a single larger meal was carried out in another set of volunteers. The fractional rate of albumin synthesis was again significantly elevated after feeding (P <0.05), rising from 7.1 (+/- 0.4)%/day in the fasted state to 9.1 (+/- 0.6)%/day in the fed state. In both studies, similar responses were observed in the absolute rate of albumin synthesis (mg day-1 kg-1). 4. Albumin secretion time was significantly shorter (P <0.05) after feeding in both studies, suggesting that the acute stimulation in albumin synthesis observed after feeding may in part be mediated via a post-transcriptional mechanism. 5. The response of total liver protein synthesis to oral feeding was investigated in an animal model employing adult rats studied with a flooding amount of [2,6-3H]phenylalanine.(ABSTRACT TRUNCATED AT 250 WORDS)

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KW - rodentia

KW - mammalia

KW - vertebrata

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JO - Clinical Science

JF - Clinical Science

SN - 0143-5221

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ER -