Adaptation of Ruminococcus flavefaciens resulting in increased degradation of ryegrass cell walls

L Saluzzi, H J Flint, C S Stewart

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

This study investigated the long term adaptation of a ruminal bacterium to growth on four different plant cell wall substrates. No significant increase in degradation was detected for lucerne. barley straw or weeping lovegrass after 23 serial subcultures of the cellulolytic rumen bacterium Ruminococcus flavefaciens strain 17 on each of these substrates. Significantly increased substrate degradation by R. flavefaciens strain 17 was however observed after 23 subcultures oil perennial ryegrass. The increase in dry matter solubilisation (from 24.3 to 39.5% in 24 h incubation and from 52.3 to 61% in 72 h uas at least partially due to an increase in solubilisation of xylose, glucose and arabinose. Enhanced growth of the adapted strains occurred on this substrate. Significant increases in xylanase and beta -xylosidase specific activities were detected but no effect was detected on xylanase profiles in zymogram analyses. Similar responses were observed for two cultures originally derived from single-colony re-isolates. The most likely explanation for the observed adaptation involves selection for mutations affecting the regulation of xylanolytic enzymes. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)131-137
Number of pages7
JournalFEMS Microbiology Ecology
Volume36
Issue number2-3
Publication statusPublished - Jul 2001

Keywords

  • xylanase
  • adaptation
  • ryegrass degradation
  • Ruminococcus flavefaciens
  • ARCTIC SVALBARD REINDEER
  • SEASONAL-CHANGES
  • TIMOTHY SILAGE
  • RUMEN BACTERIA
  • XYLANASE
  • STRAW
  • GENE
  • RUMINOCOCCUS-FLAVEFACIENS-17
  • DIGESTIBILITY
  • ENZYMES

Cite this

Adaptation of Ruminococcus flavefaciens resulting in increased degradation of ryegrass cell walls. / Saluzzi, L ; Flint, H J ; Stewart, C S .

In: FEMS Microbiology Ecology, Vol. 36, No. 2-3, 07.2001, p. 131-137.

Research output: Contribution to journalArticle

@article{9c7ec51da61f45acac35f4a509ed4849,
title = "Adaptation of Ruminococcus flavefaciens resulting in increased degradation of ryegrass cell walls",
abstract = "This study investigated the long term adaptation of a ruminal bacterium to growth on four different plant cell wall substrates. No significant increase in degradation was detected for lucerne. barley straw or weeping lovegrass after 23 serial subcultures of the cellulolytic rumen bacterium Ruminococcus flavefaciens strain 17 on each of these substrates. Significantly increased substrate degradation by R. flavefaciens strain 17 was however observed after 23 subcultures oil perennial ryegrass. The increase in dry matter solubilisation (from 24.3 to 39.5{\%} in 24 h incubation and from 52.3 to 61{\%} in 72 h uas at least partially due to an increase in solubilisation of xylose, glucose and arabinose. Enhanced growth of the adapted strains occurred on this substrate. Significant increases in xylanase and beta -xylosidase specific activities were detected but no effect was detected on xylanase profiles in zymogram analyses. Similar responses were observed for two cultures originally derived from single-colony re-isolates. The most likely explanation for the observed adaptation involves selection for mutations affecting the regulation of xylanolytic enzymes. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.",
keywords = "xylanase, adaptation, ryegrass degradation, Ruminococcus flavefaciens, ARCTIC SVALBARD REINDEER, SEASONAL-CHANGES, TIMOTHY SILAGE, RUMEN BACTERIA, XYLANASE, STRAW, GENE, RUMINOCOCCUS-FLAVEFACIENS-17, DIGESTIBILITY, ENZYMES",
author = "L Saluzzi and Flint, {H J} and Stewart, {C S}",
year = "2001",
month = "7",
language = "English",
volume = "36",
pages = "131--137",
journal = "FEMS Microbiology Ecology",
issn = "1574-6941",
publisher = "Oxford University Press",
number = "2-3",

}

TY - JOUR

T1 - Adaptation of Ruminococcus flavefaciens resulting in increased degradation of ryegrass cell walls

AU - Saluzzi, L

AU - Flint, H J

AU - Stewart, C S

PY - 2001/7

Y1 - 2001/7

N2 - This study investigated the long term adaptation of a ruminal bacterium to growth on four different plant cell wall substrates. No significant increase in degradation was detected for lucerne. barley straw or weeping lovegrass after 23 serial subcultures of the cellulolytic rumen bacterium Ruminococcus flavefaciens strain 17 on each of these substrates. Significantly increased substrate degradation by R. flavefaciens strain 17 was however observed after 23 subcultures oil perennial ryegrass. The increase in dry matter solubilisation (from 24.3 to 39.5% in 24 h incubation and from 52.3 to 61% in 72 h uas at least partially due to an increase in solubilisation of xylose, glucose and arabinose. Enhanced growth of the adapted strains occurred on this substrate. Significant increases in xylanase and beta -xylosidase specific activities were detected but no effect was detected on xylanase profiles in zymogram analyses. Similar responses were observed for two cultures originally derived from single-colony re-isolates. The most likely explanation for the observed adaptation involves selection for mutations affecting the regulation of xylanolytic enzymes. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

AB - This study investigated the long term adaptation of a ruminal bacterium to growth on four different plant cell wall substrates. No significant increase in degradation was detected for lucerne. barley straw or weeping lovegrass after 23 serial subcultures of the cellulolytic rumen bacterium Ruminococcus flavefaciens strain 17 on each of these substrates. Significantly increased substrate degradation by R. flavefaciens strain 17 was however observed after 23 subcultures oil perennial ryegrass. The increase in dry matter solubilisation (from 24.3 to 39.5% in 24 h incubation and from 52.3 to 61% in 72 h uas at least partially due to an increase in solubilisation of xylose, glucose and arabinose. Enhanced growth of the adapted strains occurred on this substrate. Significant increases in xylanase and beta -xylosidase specific activities were detected but no effect was detected on xylanase profiles in zymogram analyses. Similar responses were observed for two cultures originally derived from single-colony re-isolates. The most likely explanation for the observed adaptation involves selection for mutations affecting the regulation of xylanolytic enzymes. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

KW - xylanase

KW - adaptation

KW - ryegrass degradation

KW - Ruminococcus flavefaciens

KW - ARCTIC SVALBARD REINDEER

KW - SEASONAL-CHANGES

KW - TIMOTHY SILAGE

KW - RUMEN BACTERIA

KW - XYLANASE

KW - STRAW

KW - GENE

KW - RUMINOCOCCUS-FLAVEFACIENS-17

KW - DIGESTIBILITY

KW - ENZYMES

M3 - Article

VL - 36

SP - 131

EP - 137

JO - FEMS Microbiology Ecology

JF - FEMS Microbiology Ecology

SN - 1574-6941

IS - 2-3

ER -