Adaptation to flavomycin in the ruminal bacterium, Prevotella bryantii

J. E. Edwards, N. R. McEwan, R. J. Wallace

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Aims: The recent EU ban of growth-promoting antibiotics in animal production was based on fears concerning antibiotic resistance being transmitted to human pathogens. This paper explores the adaptation mechanism of a common ruminal bacterium, Prevotella bryantii, to one of the banned compounds, flavomycin (flavophospholipol).

Methods and Results: Growth in the presence of flavomycin (2 and 20 mu g ml(-1)) was characterized by a concentration-dependent increase in the length of the lag phase, which decreased after previous flavomycin exposure. From growth patterns on solid medium, decreased sensitivity appeared to be due to a whole-population adaptation. Proteomic analysis indicated upregulation of three native proteins occurred following flavomycin adaptation. Further analysis of two of these proteins resulted in no database matches, suggesting that they may be species-specific. Flavomycin adaptation also resulted in co-adaptation to bacitracin and vancomycin.

Conclusions: Adaptation of P. bryantii to flavomycin, which also resulted in co-adaptation to bacitracin and vancomycin, may involve an increased availability of undecaprenyl pyrophosphate.

Significance and Impact of the Study: The use of flavomycin, and similar growth-promoting antibiotics, in animal production may prompt adaptive responses in ruminal bacteria which can significantly change their antibiotic sensitivity.

Original languageEnglish
Pages (from-to)1617-1623
Number of pages7
JournalJournal of Applied Microbiology
Volume104
Issue number6
Early online date9 Jan 2008
DOIs
Publication statusPublished - Jun 2008

Keywords

  • adaptation
  • antibiotic
  • flavomycin
  • Prevotella bryantii
  • resistance
  • rumen
  • Escherichia-coli
  • persister cells
  • vancomycin resistance
  • bacitracin resistance
  • flavophospholipol
  • monensin
  • moenomycin
  • tolerance
  • mechanism

Cite this

Adaptation to flavomycin in the ruminal bacterium, Prevotella bryantii. / Edwards, J. E.; McEwan, N. R.; Wallace, R. J.

In: Journal of Applied Microbiology, Vol. 104, No. 6, 06.2008, p. 1617-1623.

Research output: Contribution to journalArticle

Edwards, J. E. ; McEwan, N. R. ; Wallace, R. J. / Adaptation to flavomycin in the ruminal bacterium, Prevotella bryantii. In: Journal of Applied Microbiology. 2008 ; Vol. 104, No. 6. pp. 1617-1623.
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