Adaptive response of neonatal sepsis-derived Group B Streptococcus to bilirubin

Richard Hansen (Corresponding Author), Sophie Gibson, Eduardo De Paiva Alves, Mark Goddard, Andrew MacLaren, Anne Marie Karcher, Susan Berry, Elaina S. R. Collie-Duguid, Emad El-Omar, Mike Munro, Georgina L. Hold

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Abstract

Hyperbilirubinemia is so common in newborns as to be termed physiological. The most common bacteria involved in early-onset neonatal sepsis are Streptococcus agalactiae, commonly called Group B Streptococcus (GBS). Whilst previous studies show bilirubin has antioxidant properties and is beneficial in endotoxic shock, little thought has been given to whether bilirubin might have antibacterial properties. In this study, we performed a transcriptomic and proteomic assessment of GBS cultured in the presence/absence of bilirubin. Our analysis revealed that increasing levels of bilirubin (>100 µmol/L) negatively correlated with GBS growth (18% reduction from 0–400 µmol/L on plate model, p < 0.001; 33% reduction from 0–100 µmol/L in liquid model, p = 0.02). Transcriptome analysis demonstrated 19 differentially expressed genes, almost exclusively up-regulated in the presence of bilirubin. Proteomic analysis identified 12 differentially expressed proteins, half over-expressed in the presence of bilirubin. Functional analysis using Gene Ontology and KEGG pathways18 revealed a differential expression of genes involved in transport and carbohydrate metabolism, suggesting bilirubin may impact on substrate utilisation. The data improve our understanding of the mechanisms modulating GBS survival in neonatal hyperbilirubinemia and suggest physiological jaundice may have an evolutionary role in protection against early-onset neonatal sepsis.
Original languageEnglish
Article number6470
Pages (from-to)1-10
Number of pages10
JournalScientific Reports
Volume8
DOIs
Publication statusPublished - 24 Apr 2018

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Streptococcus agalactiae
Bilirubin
Proteomics
Neonatal Hyperbilirubinemia
Gene Ontology
Hyperbilirubinemia
Carbohydrate Metabolism
Gene Expression Profiling
Septic Shock
Jaundice
Neonatal Sepsis
Antioxidants
Bacteria
Gene Expression
Growth
Genes

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Hansen, R., Gibson, S., De Paiva Alves, E., Goddard, M., MacLaren, A., Karcher, A. M., ... Hold, G. L. (2018). Adaptive response of neonatal sepsis-derived Group B Streptococcus to bilirubin. Scientific Reports, 8, 1-10. [6470]. https://doi.org/10.1038/s41598-018-24811-3

Adaptive response of neonatal sepsis-derived Group B Streptococcus to bilirubin. / Hansen, Richard (Corresponding Author); Gibson, Sophie; De Paiva Alves, Eduardo; Goddard, Mark; MacLaren, Andrew; Karcher, Anne Marie; Berry, Susan; Collie-Duguid, Elaina S. R.; El-Omar, Emad; Munro, Mike; Hold, Georgina L.

In: Scientific Reports, Vol. 8, 6470, 24.04.2018, p. 1-10.

Research output: Contribution to journalArticle

Hansen, R, Gibson, S, De Paiva Alves, E, Goddard, M, MacLaren, A, Karcher, AM, Berry, S, Collie-Duguid, ESR, El-Omar, E, Munro, M & Hold, GL 2018, 'Adaptive response of neonatal sepsis-derived Group B Streptococcus to bilirubin', Scientific Reports, vol. 8, 6470, pp. 1-10. https://doi.org/10.1038/s41598-018-24811-3
Hansen R, Gibson S, De Paiva Alves E, Goddard M, MacLaren A, Karcher AM et al. Adaptive response of neonatal sepsis-derived Group B Streptococcus to bilirubin. Scientific Reports. 2018 Apr 24;8:1-10. 6470. https://doi.org/10.1038/s41598-018-24811-3
Hansen, Richard ; Gibson, Sophie ; De Paiva Alves, Eduardo ; Goddard, Mark ; MacLaren, Andrew ; Karcher, Anne Marie ; Berry, Susan ; Collie-Duguid, Elaina S. R. ; El-Omar, Emad ; Munro, Mike ; Hold, Georgina L. / Adaptive response of neonatal sepsis-derived Group B Streptococcus to bilirubin. In: Scientific Reports. 2018 ; Vol. 8. pp. 1-10.
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AU - El-Omar, Emad

AU - Munro, Mike

AU - Hold, Georgina L.

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N2 - Hyperbilirubinemia is so common in newborns as to be termed physiological. The most common bacteria involved in early-onset neonatal sepsis are Streptococcus agalactiae, commonly called Group B Streptococcus (GBS). Whilst previous studies show bilirubin has antioxidant properties and is beneficial in endotoxic shock, little thought has been given to whether bilirubin might have antibacterial properties. In this study, we performed a transcriptomic and proteomic assessment of GBS cultured in the presence/absence of bilirubin. Our analysis revealed that increasing levels of bilirubin (>100 µmol/L) negatively correlated with GBS growth (18% reduction from 0–400 µmol/L on plate model, p < 0.001; 33% reduction from 0–100 µmol/L in liquid model, p = 0.02). Transcriptome analysis demonstrated 19 differentially expressed genes, almost exclusively up-regulated in the presence of bilirubin. Proteomic analysis identified 12 differentially expressed proteins, half over-expressed in the presence of bilirubin. Functional analysis using Gene Ontology and KEGG pathways18 revealed a differential expression of genes involved in transport and carbohydrate metabolism, suggesting bilirubin may impact on substrate utilisation. The data improve our understanding of the mechanisms modulating GBS survival in neonatal hyperbilirubinemia and suggest physiological jaundice may have an evolutionary role in protection against early-onset neonatal sepsis.

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