Agonist-induced internalization and trafficking of cannabinoid CB1 receptors in hippocampal neurons

Angela Alice Coutts, Sharon Anavi-Goffer, Ruth Alexandra Ross, David Joseph MacEwan, K. Mackie, Roger Guy Pertwee, A. J. Irving

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Abstract

Agonist-induced internalization of G-protein-coupled receptors is an important mechanism for regulating receptor abundance and availability at the plasma membrane. in this study we have used immunolabeling techniques and confocal microscopy to investigate agonist-induced internalization and trafficking of CB1 receptors in rat cultured hippocampal neurons. The levels of cell surface CB1 receptor immunoreactivity associated with presynaptic GABAergic terminals decreased markedly (by up to 84%) after exposure to the cannabinoid agonist (+)-WIN55212, in a concentration-dependent (0.1-1 muM) and stereoselective manner. Inhibition was maximal at 16 hr and abolished in the presence of SR141716A, a selective CB1 receptor antagonist. Methanandamide (an analog of an endogenous cannabinoid, anandamide) also reduced cell surface labeling (by 43% at 1 muM). Differential labeling of cell surface and intracellular pools of receptor demonstrated that the reduction in cell surface immunoreactivity reflects agonist-induced internalization and suggests that the internalized CB1 receptors are translocated toward the soma. The internalization process did not require activated G-protein alpha (i) or alpha (o) subunits. A different pattern of cell surface CB1 receptor expression was observed using an undifferentiated F-11 cell line, which had pronounced somatic labeling. In these cells substantial CB1 receptor internalization was also observed after exposure to (+)-WIN55212 (1 muM) for relatively short periods (30 min) of agonist exposure. In summary, this dynamic modulation of CB1 receptor expression may play an important role in the development of cannabinoid tolerance in the CNS. Agonist-induced internalization at presynaptic terminals has important implications for the modulatory effects of G-protein-coupled receptors on neurotransmitter release.

Original languageEnglish
Pages (from-to)2425-2433
Number of pages9
JournalJournal of Neuroscience
Volume21
Issue number7
Publication statusPublished - Apr 2001

Keywords

  • internalization
  • cannabinoid
  • receptor trafficking
  • CB1
  • hippocampal
  • F-11
  • dorsal-root ganglia
  • inverse agonist
  • neuroblastoma-cells
  • in-vivo
  • somatostatin statin receptor
  • messenger-RNA
  • rat-brain
  • mechanisms
  • desensitization
  • activation

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