Allelic Variation in the Heliocbacter-Pylori Flagellin Genes FLAA and FLAB: it's consequences for strain typing schemes and population-structure

Ken J. Forbes, Z FANG, Thomas Hugh Pennington

Research output: Contribution to journalArticle

Abstract

Extensive DNA sequence diversity was noted in Helicobacter pylori flagellin genes flaA and flaB. PCR amplified sequences from 49 isolates were digested with AluI, HindIII, MboI or MspI, the resultant patterns were compared between the different isolates and these used to differentiate the isolates from each other. Evidence that the extensive diversity that was found in these genes is the result of reassortment of sequences between strains in the bacterial population is presented, such that a comparatively small number of individual sequence mutations can recombine together in random combinations to form a greater number of distinct alleles. Geographical differences in the predominant patterns in the flaA alleles were also observed and could reflect regional differences either in the human host population or in the bacterial population. In view of the genetic complexity of this species, molecular typing schemes designed to identify related strains may falsely associate strains if the methods do not characterize sufficient genetic sites to exclude chance associations of genetic markers in strains which are actually not closely related to each other.

Original languageEnglish
Pages (from-to)257-266
Number of pages10
JournalEpidemiology and Infection
Volume114
Issue number2
Publication statusPublished - Apr 1995

Keywords

  • campylobacter-pylori
  • diversity
  • transformation
  • construction
  • influenzae
  • sequence
  • pathogen
  • cloning
  • genome

Cite this

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title = "Allelic Variation in the Heliocbacter-Pylori Flagellin Genes FLAA and FLAB: it's consequences for strain typing schemes and population-structure",
abstract = "Extensive DNA sequence diversity was noted in Helicobacter pylori flagellin genes flaA and flaB. PCR amplified sequences from 49 isolates were digested with AluI, HindIII, MboI or MspI, the resultant patterns were compared between the different isolates and these used to differentiate the isolates from each other. Evidence that the extensive diversity that was found in these genes is the result of reassortment of sequences between strains in the bacterial population is presented, such that a comparatively small number of individual sequence mutations can recombine together in random combinations to form a greater number of distinct alleles. Geographical differences in the predominant patterns in the flaA alleles were also observed and could reflect regional differences either in the human host population or in the bacterial population. In view of the genetic complexity of this species, molecular typing schemes designed to identify related strains may falsely associate strains if the methods do not characterize sufficient genetic sites to exclude chance associations of genetic markers in strains which are actually not closely related to each other.",
keywords = "campylobacter-pylori, diversity, transformation, construction, influenzae, sequence, pathogen, cloning, genome",
author = "Forbes, {Ken J.} and Z FANG and Pennington, {Thomas Hugh}",
year = "1995",
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journal = "Epidemiology and Infection",
issn = "0950-2688",
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TY - JOUR

T1 - Allelic Variation in the Heliocbacter-Pylori Flagellin Genes FLAA and FLAB

T2 - it's consequences for strain typing schemes and population-structure

AU - Forbes, Ken J.

AU - FANG, Z

AU - Pennington, Thomas Hugh

PY - 1995/4

Y1 - 1995/4

N2 - Extensive DNA sequence diversity was noted in Helicobacter pylori flagellin genes flaA and flaB. PCR amplified sequences from 49 isolates were digested with AluI, HindIII, MboI or MspI, the resultant patterns were compared between the different isolates and these used to differentiate the isolates from each other. Evidence that the extensive diversity that was found in these genes is the result of reassortment of sequences between strains in the bacterial population is presented, such that a comparatively small number of individual sequence mutations can recombine together in random combinations to form a greater number of distinct alleles. Geographical differences in the predominant patterns in the flaA alleles were also observed and could reflect regional differences either in the human host population or in the bacterial population. In view of the genetic complexity of this species, molecular typing schemes designed to identify related strains may falsely associate strains if the methods do not characterize sufficient genetic sites to exclude chance associations of genetic markers in strains which are actually not closely related to each other.

AB - Extensive DNA sequence diversity was noted in Helicobacter pylori flagellin genes flaA and flaB. PCR amplified sequences from 49 isolates were digested with AluI, HindIII, MboI or MspI, the resultant patterns were compared between the different isolates and these used to differentiate the isolates from each other. Evidence that the extensive diversity that was found in these genes is the result of reassortment of sequences between strains in the bacterial population is presented, such that a comparatively small number of individual sequence mutations can recombine together in random combinations to form a greater number of distinct alleles. Geographical differences in the predominant patterns in the flaA alleles were also observed and could reflect regional differences either in the human host population or in the bacterial population. In view of the genetic complexity of this species, molecular typing schemes designed to identify related strains may falsely associate strains if the methods do not characterize sufficient genetic sites to exclude chance associations of genetic markers in strains which are actually not closely related to each other.

KW - campylobacter-pylori

KW - diversity

KW - transformation

KW - construction

KW - influenzae

KW - sequence

KW - pathogen

KW - cloning

KW - genome

M3 - Article

VL - 114

SP - 257

EP - 266

JO - Epidemiology and Infection

JF - Epidemiology and Infection

SN - 0950-2688

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ER -