Alpha-tocopherol binding activity of red blood cells in smokers

M Bellizzi, A K DuttaRoy, G G Duthie, W P T James

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Despite high plasma levels of vitamin E, red blood cell membranes contain relatively low levels of vitamin E. This suggests the existence of a selective vitamin E uptake/regeneration system in human red blood cell membranes. alpha-Tocopherol binding sites on human red blood cells are thought to be involved in the uptake of alpha-tocopherol from the plasma. To understand the role of the uptake system we have compared the alpha-tocopherol content and binding activity of red blood cells from smokers and non-smokers. The specific binding of [H-3]alpha-tocopherol to pure red blood cell preparations from smokers (n = 7, 28.4 +/- 2.8 years) was 30.6 +/- 3.2 fmoles per 3 x 10(8) red blood cells and for non-smokers (n = 17, 27.9 +/- 1.3 years) was 41.7 +/- 3.7 fmoles per 3 x 10(8) red blood cells. Thus alpha-tocopherol uptake activity was significantly lower in smokers (P = 0.05). Red blood cells from smokers contained less (1.8 +/- 0.4 mu g/gHb) alpha-tocopherol than non-smokers (2.8 +/- 0.3 mu g/gHb), (P < 0.05), despite plasma levels of alpha-tocopherol being similar: 12.9 +/- 0.8 mu M in non-smokers vs. 12.7 +/- 0.5 mu M in smokers. However, adjusting plasma alpha-tocopherol for total plasma cholesterol plus triacylglycerols showed alpha-tocopherol levels were higher (P < 0.01) in non-smokers (2.84 +/- 0.10 mu mol alpha-tocopherol/mmol [cholesterol+triacylglycerol]) than in smokers (2.36 +/- 0.11 mu mol alpha-tocopherol/mmol [cholesterol+triacylglycerol]). The reduced alpha-tocopherol levels in red blood cells from smokers may be due to impairment of alpha-tocopherol uptake activity. The reduced levels of alpha-tocopherol in smokers red blood cells was not associated with any changes in cell membrane fluidity. At present it is not known whether supplementation of smokers with vitamin E would normalise the alpha-tocopherol uptake activity of red blood cells.

Original languageEnglish
Pages (from-to)105-112
Number of pages8
JournalFREE RADICAL RESEARCH
Volume27
Issue number1
Publication statusPublished - 1997

Keywords

  • smoking
  • alpha-tocopherol uptake
  • red blood cells
  • alpha-tocopherol
  • membrane fluidity
  • vitamin-E
  • erythrocyte-membranes
  • lipid-peroxidation
  • antioxidant status
  • cigarette-smoking
  • plasma
  • metabolism
  • nonsmokers
  • platelets
  • proteins

Cite this

Bellizzi, M., DuttaRoy, A. K., Duthie, G. G., & James, W. P. T. (1997). Alpha-tocopherol binding activity of red blood cells in smokers. FREE RADICAL RESEARCH, 27(1), 105-112.

Alpha-tocopherol binding activity of red blood cells in smokers. / Bellizzi, M; DuttaRoy, A K ; Duthie, G G; James, W P T .

In: FREE RADICAL RESEARCH, Vol. 27, No. 1, 1997, p. 105-112.

Research output: Contribution to journalArticle

Bellizzi, M, DuttaRoy, AK, Duthie, GG & James, WPT 1997, 'Alpha-tocopherol binding activity of red blood cells in smokers', FREE RADICAL RESEARCH, vol. 27, no. 1, pp. 105-112.
Bellizzi, M ; DuttaRoy, A K ; Duthie, G G ; James, W P T . / Alpha-tocopherol binding activity of red blood cells in smokers. In: FREE RADICAL RESEARCH. 1997 ; Vol. 27, No. 1. pp. 105-112.
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abstract = "Despite high plasma levels of vitamin E, red blood cell membranes contain relatively low levels of vitamin E. This suggests the existence of a selective vitamin E uptake/regeneration system in human red blood cell membranes. alpha-Tocopherol binding sites on human red blood cells are thought to be involved in the uptake of alpha-tocopherol from the plasma. To understand the role of the uptake system we have compared the alpha-tocopherol content and binding activity of red blood cells from smokers and non-smokers. The specific binding of [H-3]alpha-tocopherol to pure red blood cell preparations from smokers (n = 7, 28.4 +/- 2.8 years) was 30.6 +/- 3.2 fmoles per 3 x 10(8) red blood cells and for non-smokers (n = 17, 27.9 +/- 1.3 years) was 41.7 +/- 3.7 fmoles per 3 x 10(8) red blood cells. Thus alpha-tocopherol uptake activity was significantly lower in smokers (P = 0.05). Red blood cells from smokers contained less (1.8 +/- 0.4 mu g/gHb) alpha-tocopherol than non-smokers (2.8 +/- 0.3 mu g/gHb), (P < 0.05), despite plasma levels of alpha-tocopherol being similar: 12.9 +/- 0.8 mu M in non-smokers vs. 12.7 +/- 0.5 mu M in smokers. However, adjusting plasma alpha-tocopherol for total plasma cholesterol plus triacylglycerols showed alpha-tocopherol levels were higher (P < 0.01) in non-smokers (2.84 +/- 0.10 mu mol alpha-tocopherol/mmol [cholesterol+triacylglycerol]) than in smokers (2.36 +/- 0.11 mu mol alpha-tocopherol/mmol [cholesterol+triacylglycerol]). The reduced alpha-tocopherol levels in red blood cells from smokers may be due to impairment of alpha-tocopherol uptake activity. The reduced levels of alpha-tocopherol in smokers red blood cells was not associated with any changes in cell membrane fluidity. At present it is not known whether supplementation of smokers with vitamin E would normalise the alpha-tocopherol uptake activity of red blood cells.",
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AU - Bellizzi, M

AU - DuttaRoy, A K

AU - Duthie, G G

AU - James, W P T

PY - 1997

Y1 - 1997

N2 - Despite high plasma levels of vitamin E, red blood cell membranes contain relatively low levels of vitamin E. This suggests the existence of a selective vitamin E uptake/regeneration system in human red blood cell membranes. alpha-Tocopherol binding sites on human red blood cells are thought to be involved in the uptake of alpha-tocopherol from the plasma. To understand the role of the uptake system we have compared the alpha-tocopherol content and binding activity of red blood cells from smokers and non-smokers. The specific binding of [H-3]alpha-tocopherol to pure red blood cell preparations from smokers (n = 7, 28.4 +/- 2.8 years) was 30.6 +/- 3.2 fmoles per 3 x 10(8) red blood cells and for non-smokers (n = 17, 27.9 +/- 1.3 years) was 41.7 +/- 3.7 fmoles per 3 x 10(8) red blood cells. Thus alpha-tocopherol uptake activity was significantly lower in smokers (P = 0.05). Red blood cells from smokers contained less (1.8 +/- 0.4 mu g/gHb) alpha-tocopherol than non-smokers (2.8 +/- 0.3 mu g/gHb), (P < 0.05), despite plasma levels of alpha-tocopherol being similar: 12.9 +/- 0.8 mu M in non-smokers vs. 12.7 +/- 0.5 mu M in smokers. However, adjusting plasma alpha-tocopherol for total plasma cholesterol plus triacylglycerols showed alpha-tocopherol levels were higher (P < 0.01) in non-smokers (2.84 +/- 0.10 mu mol alpha-tocopherol/mmol [cholesterol+triacylglycerol]) than in smokers (2.36 +/- 0.11 mu mol alpha-tocopherol/mmol [cholesterol+triacylglycerol]). The reduced alpha-tocopherol levels in red blood cells from smokers may be due to impairment of alpha-tocopherol uptake activity. The reduced levels of alpha-tocopherol in smokers red blood cells was not associated with any changes in cell membrane fluidity. At present it is not known whether supplementation of smokers with vitamin E would normalise the alpha-tocopherol uptake activity of red blood cells.

AB - Despite high plasma levels of vitamin E, red blood cell membranes contain relatively low levels of vitamin E. This suggests the existence of a selective vitamin E uptake/regeneration system in human red blood cell membranes. alpha-Tocopherol binding sites on human red blood cells are thought to be involved in the uptake of alpha-tocopherol from the plasma. To understand the role of the uptake system we have compared the alpha-tocopherol content and binding activity of red blood cells from smokers and non-smokers. The specific binding of [H-3]alpha-tocopherol to pure red blood cell preparations from smokers (n = 7, 28.4 +/- 2.8 years) was 30.6 +/- 3.2 fmoles per 3 x 10(8) red blood cells and for non-smokers (n = 17, 27.9 +/- 1.3 years) was 41.7 +/- 3.7 fmoles per 3 x 10(8) red blood cells. Thus alpha-tocopherol uptake activity was significantly lower in smokers (P = 0.05). Red blood cells from smokers contained less (1.8 +/- 0.4 mu g/gHb) alpha-tocopherol than non-smokers (2.8 +/- 0.3 mu g/gHb), (P < 0.05), despite plasma levels of alpha-tocopherol being similar: 12.9 +/- 0.8 mu M in non-smokers vs. 12.7 +/- 0.5 mu M in smokers. However, adjusting plasma alpha-tocopherol for total plasma cholesterol plus triacylglycerols showed alpha-tocopherol levels were higher (P < 0.01) in non-smokers (2.84 +/- 0.10 mu mol alpha-tocopherol/mmol [cholesterol+triacylglycerol]) than in smokers (2.36 +/- 0.11 mu mol alpha-tocopherol/mmol [cholesterol+triacylglycerol]). The reduced alpha-tocopherol levels in red blood cells from smokers may be due to impairment of alpha-tocopherol uptake activity. The reduced levels of alpha-tocopherol in smokers red blood cells was not associated with any changes in cell membrane fluidity. At present it is not known whether supplementation of smokers with vitamin E would normalise the alpha-tocopherol uptake activity of red blood cells.

KW - smoking

KW - alpha-tocopherol uptake

KW - red blood cells

KW - alpha-tocopherol

KW - membrane fluidity

KW - vitamin-E

KW - erythrocyte-membranes

KW - lipid-peroxidation

KW - antioxidant status

KW - cigarette-smoking

KW - plasma

KW - metabolism

KW - nonsmokers

KW - platelets

KW - proteins

M3 - Article

VL - 27

SP - 105

EP - 112

JO - Free Radical Research

JF - Free Radical Research

SN - 1029-2470

IS - 1

ER -