Alteration of aortic function from streptozotocin-diabetic rats with Kilham's virus is associated with inducible nitric oxide synthase

Matthew Robert Nangle, Mary Anne Cotter, Norman E Cameron

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Kilham’s rat virus (KRV) is a parvovirus commonly known to affect laboratory rats. Qualitative immunohistochemical analysis revealed that aorta isolated from KRV-infected streptozotocin (STZ)-induced diabetic adult rats expressed markedly greater levels of inducible nitric oxide synthase (iNOS) than aorta from KRV-infected controls. In contrast with the prevailing literature, nitric oxide-mediated endothelium-dependent relaxation to acetylcholine was not blunted by STZ-diabetes, but was comparable to relaxations of aorta from controls. However, with increasing ex vivo duration, a decreased response to acetylcholine was observed in the STZ-diabetic aorta. In addition, whereas contraction responses to phenylephrine were not significantly altered over time in control tissue, aorta from STZ-diabetic rats developed increased tensions. The data suggest that increased iNOS-derived nitric oxide masks expected acetylcholine-mediated relaxation deficits as a result of KRV-infection, and that the deficit is unmasked by iNOS turnover ex vivo.

Original languageEnglish
Pages (from-to)455-459
Number of pages5
JournalVeterinary Journal
Issue number3
Early online date24 Oct 2005
Publication statusPublished - Nov 2006


  • aorta
  • diabetes mellitus
  • Kilham's rat virus
  • inducible nitric oxide synthase
  • endothelium

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