Alterations in the abundance and co-occurrence of Akkermansia muciniphila and Faecalibacterium prausnitzii in the colonic mucosa of inflammatory bowel disease subjects

Mireia Lopez-Siles, Núria Enrich-Capó, Xavier Aldeguer, Miriam Sabat-Mir, Sylvia Duncan, Jesús Garcia-Gil, Margarita Martinez-Medina

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Abstract

Akkermansia muciniphila and Faecalibacterium prausnitzii, cohabitants in the intestinal mucosa, are considered members of a healthy microbiota and reduction of both species occurs in several intestinal disorders, including inflammatory bowel disease. Little is known however about a possible link between the reduction in quantity of these species, and in which circumstances this may occur. This study aims to determine the abundances and co-occurrence of the two species in order to elucidate conditions that may compromise their presence in the gut. Loads of A. muciniphila, total F. prausnitzii (16S rRNA gene copies) and its two phylogroup were determined by quantitative polymerase chain reaction targeting specific regions of the 16S rRNA gene in colonic biopsies from 17 healthy controls (H), 23 patients with ulcerative colitis (UC) and 31 patients with Crohn’s disease (CD). Data were normalised to total bacterial 16S rRNA gene copies in the same sample. Prevalence, relative abundances and correlation analyses were performed according to type of disease and considering relevant clinical characteristics of patients such as IBD location, age of disease onset, CD behaviour, current medication and activity status. Co-occurrence of both species was found in 29% of H, 65% of UC and 29% of CD. Lower levels of total F. prausnitzii and phylogroups were found in subjects with CD, compared with H subjects (P≤0.044). In contrast, no differences were found with the regard to A. muciniphila abundance across different disease states,but CD patients with disease onset below 16 years of age featured a marked depletion of this species. In CD patients, correlation between A. muciniphila and total F. prausnitzii (ρ=0.362, P=0.045) was observed, and particularly in those with non-stricturing, non-penetrating disease behaviour and under moderate immunosuppressants therapy. Altogether, this study revealed that co-occurrence of both species differs between disease status. In addition, IBD patients featured a reduction of F. prausnitzii but similar loads of A. muciniphila when compared to H subjects, with the exception of those with early onset CD. Depletion of A. muciniphila in this subgroup of subjects suggests that it could be a potential biomarker to assist in paediatric CD diagnosis.
Original languageEnglish
Article number281
Number of pages16
JournalFrontiers in cellular and infection microbiology
Volume8
Early online date31 Aug 2018
DOIs
Publication statusPublished - 7 Sep 2018

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Inflammatory Bowel Diseases
Crohn Disease
Mucous Membrane
rRNA Genes
Ulcerative Colitis
Microbiota
Intestinal Mucosa
Immunosuppressive Agents
Faecalibacterium prausnitzii
Age of Onset
Biomarkers
Biopsy
Polymerase Chain Reaction

Keywords

  • Akkermansia muciniphila
  • Faecalibacterium prausnitzii
  • Crohn’s disease
  • Ulcerative Colitis
  • Inflammatory Bowel Diseases

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Alterations in the abundance and co-occurrence of Akkermansia muciniphila and Faecalibacterium prausnitzii in the colonic mucosa of inflammatory bowel disease subjects. / Lopez-Siles, Mireia; Enrich-Capó, Núria ; Aldeguer, Xavier; Sabat-Mir, Miriam; Duncan, Sylvia; Garcia-Gil, Jesús; Martinez-Medina, Margarita.

In: Frontiers in cellular and infection microbiology, Vol. 8, 281, 07.09.2018.

Research output: Contribution to journalArticle

Lopez-Siles, Mireia ; Enrich-Capó, Núria ; Aldeguer, Xavier ; Sabat-Mir, Miriam ; Duncan, Sylvia ; Garcia-Gil, Jesús ; Martinez-Medina, Margarita. / Alterations in the abundance and co-occurrence of Akkermansia muciniphila and Faecalibacterium prausnitzii in the colonic mucosa of inflammatory bowel disease subjects. In: Frontiers in cellular and infection microbiology. 2018 ; Vol. 8.
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abstract = "Akkermansia muciniphila and Faecalibacterium prausnitzii, cohabitants in the intestinal mucosa, are considered members of a healthy microbiota and reduction of both species occurs in several intestinal disorders, including inflammatory bowel disease. Little is known however about a possible link between the reduction in quantity of these species, and in which circumstances this may occur. This study aims to determine the abundances and co-occurrence of the two species in order to elucidate conditions that may compromise their presence in the gut. Loads of A. muciniphila, total F. prausnitzii (16S rRNA gene copies) and its two phylogroup were determined by quantitative polymerase chain reaction targeting specific regions of the 16S rRNA gene in colonic biopsies from 17 healthy controls (H), 23 patients with ulcerative colitis (UC) and 31 patients with Crohn’s disease (CD). Data were normalised to total bacterial 16S rRNA gene copies in the same sample. Prevalence, relative abundances and correlation analyses were performed according to type of disease and considering relevant clinical characteristics of patients such as IBD location, age of disease onset, CD behaviour, current medication and activity status. Co-occurrence of both species was found in 29{\%} of H, 65{\%} of UC and 29{\%} of CD. Lower levels of total F. prausnitzii and phylogroups were found in subjects with CD, compared with H subjects (P≤0.044). In contrast, no differences were found with the regard to A. muciniphila abundance across different disease states,but CD patients with disease onset below 16 years of age featured a marked depletion of this species. In CD patients, correlation between A. muciniphila and total F. prausnitzii (ρ=0.362, P=0.045) was observed, and particularly in those with non-stricturing, non-penetrating disease behaviour and under moderate immunosuppressants therapy. Altogether, this study revealed that co-occurrence of both species differs between disease status. In addition, IBD patients featured a reduction of F. prausnitzii but similar loads of A. muciniphila when compared to H subjects, with the exception of those with early onset CD. Depletion of A. muciniphila in this subgroup of subjects suggests that it could be a potential biomarker to assist in paediatric CD diagnosis.",
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T1 - Alterations in the abundance and co-occurrence of Akkermansia muciniphila and Faecalibacterium prausnitzii in the colonic mucosa of inflammatory bowel disease subjects

AU - Lopez-Siles, Mireia

AU - Enrich-Capó, Núria

AU - Aldeguer, Xavier

AU - Sabat-Mir, Miriam

AU - Duncan, Sylvia

AU - Garcia-Gil, Jesús

AU - Martinez-Medina, Margarita

N1 - This work was funded by the Universitat de Girona projects MPCUdG2016-009 and GdRCompetUdG2017, and the Spanish Ministry of Education and Science through projects SAF2006-00414, SAF2010-15896 and SAF2013-43284-P, being the last co-funded by the European Regional Development. Dr. Sylvia H. Duncan acknowledges support from the Scottish Government Research and Environment Science and Analytical Services Division (RESAS).

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N2 - Akkermansia muciniphila and Faecalibacterium prausnitzii, cohabitants in the intestinal mucosa, are considered members of a healthy microbiota and reduction of both species occurs in several intestinal disorders, including inflammatory bowel disease. Little is known however about a possible link between the reduction in quantity of these species, and in which circumstances this may occur. This study aims to determine the abundances and co-occurrence of the two species in order to elucidate conditions that may compromise their presence in the gut. Loads of A. muciniphila, total F. prausnitzii (16S rRNA gene copies) and its two phylogroup were determined by quantitative polymerase chain reaction targeting specific regions of the 16S rRNA gene in colonic biopsies from 17 healthy controls (H), 23 patients with ulcerative colitis (UC) and 31 patients with Crohn’s disease (CD). Data were normalised to total bacterial 16S rRNA gene copies in the same sample. Prevalence, relative abundances and correlation analyses were performed according to type of disease and considering relevant clinical characteristics of patients such as IBD location, age of disease onset, CD behaviour, current medication and activity status. Co-occurrence of both species was found in 29% of H, 65% of UC and 29% of CD. Lower levels of total F. prausnitzii and phylogroups were found in subjects with CD, compared with H subjects (P≤0.044). In contrast, no differences were found with the regard to A. muciniphila abundance across different disease states,but CD patients with disease onset below 16 years of age featured a marked depletion of this species. In CD patients, correlation between A. muciniphila and total F. prausnitzii (ρ=0.362, P=0.045) was observed, and particularly in those with non-stricturing, non-penetrating disease behaviour and under moderate immunosuppressants therapy. Altogether, this study revealed that co-occurrence of both species differs between disease status. In addition, IBD patients featured a reduction of F. prausnitzii but similar loads of A. muciniphila when compared to H subjects, with the exception of those with early onset CD. Depletion of A. muciniphila in this subgroup of subjects suggests that it could be a potential biomarker to assist in paediatric CD diagnosis.

AB - Akkermansia muciniphila and Faecalibacterium prausnitzii, cohabitants in the intestinal mucosa, are considered members of a healthy microbiota and reduction of both species occurs in several intestinal disorders, including inflammatory bowel disease. Little is known however about a possible link between the reduction in quantity of these species, and in which circumstances this may occur. This study aims to determine the abundances and co-occurrence of the two species in order to elucidate conditions that may compromise their presence in the gut. Loads of A. muciniphila, total F. prausnitzii (16S rRNA gene copies) and its two phylogroup were determined by quantitative polymerase chain reaction targeting specific regions of the 16S rRNA gene in colonic biopsies from 17 healthy controls (H), 23 patients with ulcerative colitis (UC) and 31 patients with Crohn’s disease (CD). Data were normalised to total bacterial 16S rRNA gene copies in the same sample. Prevalence, relative abundances and correlation analyses were performed according to type of disease and considering relevant clinical characteristics of patients such as IBD location, age of disease onset, CD behaviour, current medication and activity status. Co-occurrence of both species was found in 29% of H, 65% of UC and 29% of CD. Lower levels of total F. prausnitzii and phylogroups were found in subjects with CD, compared with H subjects (P≤0.044). In contrast, no differences were found with the regard to A. muciniphila abundance across different disease states,but CD patients with disease onset below 16 years of age featured a marked depletion of this species. In CD patients, correlation between A. muciniphila and total F. prausnitzii (ρ=0.362, P=0.045) was observed, and particularly in those with non-stricturing, non-penetrating disease behaviour and under moderate immunosuppressants therapy. Altogether, this study revealed that co-occurrence of both species differs between disease status. In addition, IBD patients featured a reduction of F. prausnitzii but similar loads of A. muciniphila when compared to H subjects, with the exception of those with early onset CD. Depletion of A. muciniphila in this subgroup of subjects suggests that it could be a potential biomarker to assist in paediatric CD diagnosis.

KW - Akkermansia muciniphila

KW - Faecalibacterium prausnitzii

KW - Crohn’s disease

KW - Ulcerative Colitis

KW - Inflammatory Bowel Diseases

U2 - 10.3389/fcimb.2018.00281

DO - 10.3389/fcimb.2018.00281

M3 - Article

VL - 8

JO - Frontiers in cellular and infection microbiology

JF - Frontiers in cellular and infection microbiology

SN - 2235-2988

M1 - 281

ER -