Alterations in the concentration of an apolipoprotein E-containing subfraction of plasma high density lipoprotein in coronary heart disease

Heather M. Wilson*, Jitendra C. Patel, Daphne Russell, E. Roy Skinner

*Corresponding author for this work

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

The concentrations of high density lipoprotein (HDL) subfractions in 100 healthy male subjects were compared with 100 newly presenting patients with myocardial infarction (MI) within 12 h of the onset of chest pain. A subfraction of HDL enriched in apolipoprotein E (apo E), separated by heparin-Sepharose affinity chromotography, was present in lower concentrations (P < 0.001) in the plasma of the coronary patients than in the control subjects. This finding was confirmed by a lower content (P < 0.02) of apo E, measured by ELISA, in the total HDL fraction isolated from the coronary patients. Gradient gel electrophoresis of the total HDL demonstrated that the coronary patients had a significantly decreased concentration of the large HDL particles, HDL2b, of mean diameter 10.57 nm and a higher concentration of the smaller-sized HDL3, especially HDL3c, of mean diameter 7.62 nm. The coronary patients had a lower concentration of HDL cholesterol than the control subjects, attributable to the HDL2 fraction, with no difference in HDL2a between the two groups. There was no difference in the concentration of plasma cholesterol or triglyceride. The distribution of apo E phenotypes was similar in the two groups. HDL2b produced the highest discriminant power between the two groups, followed by apo E-rich HDL, HDL2 and HDL3c Plasma cholesterol correlated strongly with apo E-rich HDL for control subjects but not for MI survivors. This study demonstrates that the inverse relationship between HDL cholesterol and coronary risk shown in epidemiological studies is attributable to the large, apo E-containing HDL subspecies which under some circumstances are implicated in cholesterol removal by reverse cholesterol transport. This study also suggests that the concentration of the large, ago E-containing HDL may provide a sensitive predictor for subjects at risk of developing coronary heart disease.

Original languageEnglish
Pages (from-to)175-187
Number of pages13
JournalCLINICA CHIMICA ACTA
Volume220
Issue number2
DOIs
Publication statusPublished - 15 Nov 1993

Fingerprint

Apolipoproteins E
HDL Lipoproteins
Coronary Disease
Plasmas
Cholesterol
HDL Cholesterol
Myocardial Infarction
HDL2 Lipoprotein
Chest Pain
Survivors
Electrophoresis
Epidemiologic Studies
Healthy Volunteers
Triglycerides
Gels
Enzyme-Linked Immunosorbent Assay
Phenotype

Keywords

  • Coronary heart disease
  • Coronary protection
  • HDL
  • High density lipoprotein

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Alterations in the concentration of an apolipoprotein E-containing subfraction of plasma high density lipoprotein in coronary heart disease. / Wilson, Heather M.; Patel, Jitendra C.; Russell, Daphne; Skinner, E. Roy.

In: CLINICA CHIMICA ACTA, Vol. 220, No. 2, 15.11.1993, p. 175-187.

Research output: Contribution to journalArticle

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