Altered L-selectin expression in lymphocytes and increased adhesion to endothelium in patients with diabetic retinopathy

J. R. MacKinnon, R. M. Knott, John Vincent Forrester

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Aim: To investigate L-selectin expression and shedding in patients with and without retinopathy and to determine if any observed changes are reflected by a functional change in the adhesion of leucocytes to an endothelial monolayer.

Methods: Age matched diabetic patients ( 26 with retinopathy, 19 without retinopathy) were compared to 24 nondiabetic controls to determine L-selectin surface protein expression, L-selectin mRNA production, and serum L-selectin levels by flow cytometry, RT-PCR, and ELISA, respectively. An adhesion assay was used to determine the binding of lymphocytes from the respective test groups to a monolayer of human endothelial cells.

Results: Significantly reduced (p = 0.004) L-selectin expression was demonstrated on lymphocytes (CD3+) from patients with diabetes compared to controls, the lowest levels being found in those with diabetic retinopathy ( p = 0.004). L-selectin mRNA levels (p = 0.007) were significantly higher in the retinopathy group than in the no retinopathy group. Serum L-selectin levels were significantly higher (p = 0.04) in those with retinopathy compared to controls. Lymphocyte adhesion relative to control (100%) was essentially unchanged (84.0% (SD 27.7%), p = 0.15) for diabetic patients with no retinopathy and was markedly increased (192% (37.6%)) for those with retinopathy (p = 0.0001).

Conclusion: Lymphocyte activation, reduced surface L-selectin, increased circulating L-selectin, and a corresponding increase in adhesion of patients' cells using an in vitro assay, is evident in people with diabetic retinopathy. This suggests a role for lymphocyte activation in the pathogenesis of diabetic retinopathy.

Original languageEnglish
Pages (from-to)1137-1141
Number of pages4
JournalBritish Journal of Ophthalmology
Volume88
Issue number9
DOIs
Publication statusPublished - 2004

Keywords

  • DISEASE-ACTIVITY
  • MOLECULES
  • MELLITUS
  • LEUKOSTASIS
  • LEUKOCYTES
  • MONOCYTES
  • RETINA
  • CELLS

Cite this

Altered L-selectin expression in lymphocytes and increased adhesion to endothelium in patients with diabetic retinopathy. / MacKinnon, J. R.; Knott, R. M.; Forrester, John Vincent.

In: British Journal of Ophthalmology, Vol. 88, No. 9, 2004, p. 1137-1141.

Research output: Contribution to journalArticle

MacKinnon, J. R. ; Knott, R. M. ; Forrester, John Vincent. / Altered L-selectin expression in lymphocytes and increased adhesion to endothelium in patients with diabetic retinopathy. In: British Journal of Ophthalmology. 2004 ; Vol. 88, No. 9. pp. 1137-1141.
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abstract = "Aim: To investigate L-selectin expression and shedding in patients with and without retinopathy and to determine if any observed changes are reflected by a functional change in the adhesion of leucocytes to an endothelial monolayer.Methods: Age matched diabetic patients ( 26 with retinopathy, 19 without retinopathy) were compared to 24 nondiabetic controls to determine L-selectin surface protein expression, L-selectin mRNA production, and serum L-selectin levels by flow cytometry, RT-PCR, and ELISA, respectively. An adhesion assay was used to determine the binding of lymphocytes from the respective test groups to a monolayer of human endothelial cells.Results: Significantly reduced (p = 0.004) L-selectin expression was demonstrated on lymphocytes (CD3+) from patients with diabetes compared to controls, the lowest levels being found in those with diabetic retinopathy ( p = 0.004). L-selectin mRNA levels (p = 0.007) were significantly higher in the retinopathy group than in the no retinopathy group. Serum L-selectin levels were significantly higher (p = 0.04) in those with retinopathy compared to controls. Lymphocyte adhesion relative to control (100{\%}) was essentially unchanged (84.0{\%} (SD 27.7{\%}), p = 0.15) for diabetic patients with no retinopathy and was markedly increased (192{\%} (37.6{\%})) for those with retinopathy (p = 0.0001).Conclusion: Lymphocyte activation, reduced surface L-selectin, increased circulating L-selectin, and a corresponding increase in adhesion of patients' cells using an in vitro assay, is evident in people with diabetic retinopathy. This suggests a role for lymphocyte activation in the pathogenesis of diabetic retinopathy.",
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AU - MacKinnon, J. R.

AU - Knott, R. M.

AU - Forrester, John Vincent

PY - 2004

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N2 - Aim: To investigate L-selectin expression and shedding in patients with and without retinopathy and to determine if any observed changes are reflected by a functional change in the adhesion of leucocytes to an endothelial monolayer.Methods: Age matched diabetic patients ( 26 with retinopathy, 19 without retinopathy) were compared to 24 nondiabetic controls to determine L-selectin surface protein expression, L-selectin mRNA production, and serum L-selectin levels by flow cytometry, RT-PCR, and ELISA, respectively. An adhesion assay was used to determine the binding of lymphocytes from the respective test groups to a monolayer of human endothelial cells.Results: Significantly reduced (p = 0.004) L-selectin expression was demonstrated on lymphocytes (CD3+) from patients with diabetes compared to controls, the lowest levels being found in those with diabetic retinopathy ( p = 0.004). L-selectin mRNA levels (p = 0.007) were significantly higher in the retinopathy group than in the no retinopathy group. Serum L-selectin levels were significantly higher (p = 0.04) in those with retinopathy compared to controls. Lymphocyte adhesion relative to control (100%) was essentially unchanged (84.0% (SD 27.7%), p = 0.15) for diabetic patients with no retinopathy and was markedly increased (192% (37.6%)) for those with retinopathy (p = 0.0001).Conclusion: Lymphocyte activation, reduced surface L-selectin, increased circulating L-selectin, and a corresponding increase in adhesion of patients' cells using an in vitro assay, is evident in people with diabetic retinopathy. This suggests a role for lymphocyte activation in the pathogenesis of diabetic retinopathy.

AB - Aim: To investigate L-selectin expression and shedding in patients with and without retinopathy and to determine if any observed changes are reflected by a functional change in the adhesion of leucocytes to an endothelial monolayer.Methods: Age matched diabetic patients ( 26 with retinopathy, 19 without retinopathy) were compared to 24 nondiabetic controls to determine L-selectin surface protein expression, L-selectin mRNA production, and serum L-selectin levels by flow cytometry, RT-PCR, and ELISA, respectively. An adhesion assay was used to determine the binding of lymphocytes from the respective test groups to a monolayer of human endothelial cells.Results: Significantly reduced (p = 0.004) L-selectin expression was demonstrated on lymphocytes (CD3+) from patients with diabetes compared to controls, the lowest levels being found in those with diabetic retinopathy ( p = 0.004). L-selectin mRNA levels (p = 0.007) were significantly higher in the retinopathy group than in the no retinopathy group. Serum L-selectin levels were significantly higher (p = 0.04) in those with retinopathy compared to controls. Lymphocyte adhesion relative to control (100%) was essentially unchanged (84.0% (SD 27.7%), p = 0.15) for diabetic patients with no retinopathy and was markedly increased (192% (37.6%)) for those with retinopathy (p = 0.0001).Conclusion: Lymphocyte activation, reduced surface L-selectin, increased circulating L-selectin, and a corresponding increase in adhesion of patients' cells using an in vitro assay, is evident in people with diabetic retinopathy. This suggests a role for lymphocyte activation in the pathogenesis of diabetic retinopathy.

KW - DISEASE-ACTIVITY

KW - MOLECULES

KW - MELLITUS

KW - LEUKOSTASIS

KW - LEUKOCYTES

KW - MONOCYTES

KW - RETINA

KW - CELLS

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DO - 10.1136/bjo.2003.040329

M3 - Article

VL - 88

SP - 1137

EP - 1141

JO - British Journal of Ophthalmology

JF - British Journal of Ophthalmology

SN - 0007-1161

IS - 9

ER -