Altitude ammonia-oxidizing bacteria and archaea in soils of Mount Everest

Li-Mei Zhang, Mu Wang, James I Prosser, Yuan-Ming Zheng, Ji-Zheng He

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

To determine the abundance and distribution of bacterial and archaeal ammonia oxidizers in alpine and permafrost soils, 12 soils at altitudes of 4000-6550 m above sea level (m a.s.l.) were collected from the northern slope of the Mount Everest (Tibetan Plateau), where the permanent snow line is at 5800-6000 m a.s.l. Communities were characterized by real-time PCR and clone sequencing by targeting on amoA genes, which putatively encode ammonia monooxygenase subunit A. Archaeal amoA abundance was greater than bacterial amoA abundance in lower altitude soils (or=5700 m a.s.l.). Both archaeal and bacterial amoA abundance decreased abruptly in higher altitude soils. Communities shifted from a Nitrosospira amoA cluster 3a-dominated ammonia-oxidizing bacteria community in lower altitude soils to communities dominated by a newly designated Nitrosospira ME and cluster 2-related groups and Nitrosomonas cluster 6 in higher altitude soils. All archaeal amoA sequences fell within soil and sediment clusters, and the proportions of the major archaeal amoA clusters changed between the lower altitude and the higher altitude soils. These findings imply that the shift in the relative abundance and community structure of archaeal and bacterial ammonia oxidizers may result from selection of organisms adapted to altitude-dependent environmental factors in elevated soils.
Original languageEnglish
Pages (from-to)208-217
Number of pages10
JournalFEMS Microbiology Ecology
Volume70
Issue number2
Early online date1 Sep 2009
DOIs
Publication statusPublished - Nov 2009

Fingerprint

Archaea
Ammonia
Soil
ammonia
Bacteria
bacterium
soil
Oceans and Seas
sea level
Nitrosomonas
Bacterial Structures
Snow
Mercaptoethanol
Real-Time Polymerase Chain Reaction
permafrost
targeting
Clone Cells
clone
relative abundance
community structure

Keywords

  • altitude
  • ammonia
  • archaea
  • bacteria
  • biodiversity
  • cloning, molecular
  • DNA, archaeal
  • DNA, bacterial
  • ecosystem
  • genes, archaeal
  • genes, bacterial
  • oxidoreductases
  • sequence analysis, DNA
  • soil
  • soil microbiology

Cite this

Altitude ammonia-oxidizing bacteria and archaea in soils of Mount Everest. / Zhang, Li-Mei; Wang, Mu; Prosser, James I; Zheng, Yuan-Ming; He, Ji-Zheng.

In: FEMS Microbiology Ecology, Vol. 70, No. 2, 11.2009, p. 208-217.

Research output: Contribution to journalArticle

Zhang, Li-Mei ; Wang, Mu ; Prosser, James I ; Zheng, Yuan-Ming ; He, Ji-Zheng. / Altitude ammonia-oxidizing bacteria and archaea in soils of Mount Everest. In: FEMS Microbiology Ecology. 2009 ; Vol. 70, No. 2. pp. 208-217.
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