Ammonia concentration determines differential growth of ammonia-oxidising archaea and bacteria in soil microcosms

Daniel T Verhamme, James I Prosser, Graeme W Nicol

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Abstract

The first step of nitrification, oxidation of ammonia to nitrite, is performed by both ammonia-oxidising archaea (AOA) and ammonia-oxidising bacteria (AOB) in soil, but their relative contributions to ammonia oxidation and existence in distinct ecological niches remain to be determined. To determine whether available ammonia concentration has a differential effect on AOA and AOB growth, soil microcosms were incubated for 28 days with ammonium at three concentrations: native (control), intermediate (20 mu g NH(4)(+)-N per gram of soil) and high (200 mu g NH(4)(+)-N per gram of soil). Quantitative PCR demonstrated growth of AOA at all concentrations, whereas AOB growth was prominent only at the highest concentration. Similarly, denaturing gradient gel electrophoresis (DGGE) analysis revealed changes in AOA communities at all ammonium concentrations, whereas AOB communities changed significantly only at the highest ammonium concentration. These results provide evidence that ammonia concentration contributes to the definition of distinct ecological niches of AOA and AOB in soil. The ISME Journal (2011) 5, 1067-1071; doi:10.1038/ismej.2010.191; published online 13 January 2011 Subject Category: microbial ecology and functional diversity of natural habitats

Original languageEnglish
Pages (from-to)1067-1071
Number of pages5
JournalThe ISME Journal
Volume5
Issue number6
DOIs
Publication statusPublished - Jan 2011

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Keywords

  • ammonia-oxidising archaea
  • ammonia-oxidising bacteria
  • nitrification
  • ammonia concentration
  • amoA
  • grassland soils
  • oxidation
  • diversity
  • crenarchaeota
  • abundance
  • kinetics

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