AMMONIA OXIDATION AT LOW PH BY ATTACHED POPULATIONS OF NITRIFYING BACTERIA

S M ALLISON, James Ivor Prosser

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

Biofilm populations of Nitrosomonas europaea were formed in continuous flow sand columns supplied with defined inorganic medium containing 50 mug NH4+-N ml-1. Steady-state nitrite concentrations in effluent from the columns decreased as the pH of the inflowing medium was reduced but ammonia oxidation occurred at a pH value of 6, even though the pH minimum for growth of the organism in liquid batch culture was 7. No evidence was obtained for NH3 limitation at low pH values, resulting from ionization to NH4+. The pH minimum for ammonia-oxidizing activity was also reduced in continuous flow vermiculite columns supplied with medium of pH 5.4, with an effluent pH of 6.3, but detailed assessment of the effects of low pH on activity was prevented by the strong buffering capacity of vermiculite. A multispecies, nitrifying biofilm, formed as wall growth in an ammonia-limited chemostat, was capable of ammonia-oxidizing activity, but not growth, at pH 5 while growth was possible at pH 5.5. The data provide evidence of the potential for autotrophic nitrification in acid soils.

Original languageEnglish
Pages (from-to)935-941
Number of pages7
JournalSoil Biology and Biochemistry
Volume25
Issue number7
Publication statusPublished - Jul 1993

Keywords

  • ACID FOREST SOIL
  • NITROSOMONAS-EUROPAEA
  • AUTOTROPHIC NITRIFICATION
  • HEATH SOIL
  • GROWTH
  • NITROBACTER

Cite this

AMMONIA OXIDATION AT LOW PH BY ATTACHED POPULATIONS OF NITRIFYING BACTERIA. / ALLISON, S M ; Prosser, James Ivor.

In: Soil Biology and Biochemistry, Vol. 25, No. 7, 07.1993, p. 935-941.

Research output: Contribution to journalArticle

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title = "AMMONIA OXIDATION AT LOW PH BY ATTACHED POPULATIONS OF NITRIFYING BACTERIA",
abstract = "Biofilm populations of Nitrosomonas europaea were formed in continuous flow sand columns supplied with defined inorganic medium containing 50 mug NH4+-N ml-1. Steady-state nitrite concentrations in effluent from the columns decreased as the pH of the inflowing medium was reduced but ammonia oxidation occurred at a pH value of 6, even though the pH minimum for growth of the organism in liquid batch culture was 7. No evidence was obtained for NH3 limitation at low pH values, resulting from ionization to NH4+. The pH minimum for ammonia-oxidizing activity was also reduced in continuous flow vermiculite columns supplied with medium of pH 5.4, with an effluent pH of 6.3, but detailed assessment of the effects of low pH on activity was prevented by the strong buffering capacity of vermiculite. A multispecies, nitrifying biofilm, formed as wall growth in an ammonia-limited chemostat, was capable of ammonia-oxidizing activity, but not growth, at pH 5 while growth was possible at pH 5.5. The data provide evidence of the potential for autotrophic nitrification in acid soils.",
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T1 - AMMONIA OXIDATION AT LOW PH BY ATTACHED POPULATIONS OF NITRIFYING BACTERIA

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AU - Prosser, James Ivor

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N2 - Biofilm populations of Nitrosomonas europaea were formed in continuous flow sand columns supplied with defined inorganic medium containing 50 mug NH4+-N ml-1. Steady-state nitrite concentrations in effluent from the columns decreased as the pH of the inflowing medium was reduced but ammonia oxidation occurred at a pH value of 6, even though the pH minimum for growth of the organism in liquid batch culture was 7. No evidence was obtained for NH3 limitation at low pH values, resulting from ionization to NH4+. The pH minimum for ammonia-oxidizing activity was also reduced in continuous flow vermiculite columns supplied with medium of pH 5.4, with an effluent pH of 6.3, but detailed assessment of the effects of low pH on activity was prevented by the strong buffering capacity of vermiculite. A multispecies, nitrifying biofilm, formed as wall growth in an ammonia-limited chemostat, was capable of ammonia-oxidizing activity, but not growth, at pH 5 while growth was possible at pH 5.5. The data provide evidence of the potential for autotrophic nitrification in acid soils.

AB - Biofilm populations of Nitrosomonas europaea were formed in continuous flow sand columns supplied with defined inorganic medium containing 50 mug NH4+-N ml-1. Steady-state nitrite concentrations in effluent from the columns decreased as the pH of the inflowing medium was reduced but ammonia oxidation occurred at a pH value of 6, even though the pH minimum for growth of the organism in liquid batch culture was 7. No evidence was obtained for NH3 limitation at low pH values, resulting from ionization to NH4+. The pH minimum for ammonia-oxidizing activity was also reduced in continuous flow vermiculite columns supplied with medium of pH 5.4, with an effluent pH of 6.3, but detailed assessment of the effects of low pH on activity was prevented by the strong buffering capacity of vermiculite. A multispecies, nitrifying biofilm, formed as wall growth in an ammonia-limited chemostat, was capable of ammonia-oxidizing activity, but not growth, at pH 5 while growth was possible at pH 5.5. The data provide evidence of the potential for autotrophic nitrification in acid soils.

KW - ACID FOREST SOIL

KW - NITROSOMONAS-EUROPAEA

KW - AUTOTROPHIC NITRIFICATION

KW - HEATH SOIL

KW - GROWTH

KW - NITROBACTER

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JO - Soil Biology and Biochemistry

JF - Soil Biology and Biochemistry

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