Ammonium supply rate influences archaeal and bacterial ammonia oxidizers in a wetland soil vertical profile

Špela Höfferle, Graeme W Nicol, Levin Pal, Janez Hacin, James I Prosser, Ines Mandic-Mulec

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Oxidation of ammonia, the first step in nitrification, is carried out in soil by bacterial and archaeal ammonia oxidizers and recent studies suggest possible selection for the latter in low-ammonium environments. In this study, we investigated the selection of ammonia-oxidizing archaea and bacteria in wetland soil vertical profiles at two sites differing in terms of the ammonium supply rate, but not significantly in terms of the groundwater level. One site received ammonium through decomposition of organic matter, while the second, polluted site received a greater supply, through constant leakage of an underground septic tank. Soil nitrification potential was significantly greater at the polluted site. Quantification of amoA genes demonstrated greater abundance of bacterial than archaeal amoA genes throughout the soil profile at the polluted site, whereas bacterial amoA genes at the unpolluted site were below the detection limit. At both sites, archaeal, but not the bacterial community structure was clearly stratified with depth, with regard to the soil redox potential imposed by groundwater level. However, depth-related changes in the archaeal community structure may also be associated with physiological functions other than ammonia oxidation.
Original languageEnglish
Pages (from-to)302-315
Number of pages14
JournalFEMS Microbiology Ecology
Volume74
Issue number2
Early online date4 Aug 2010
DOIs
Publication statusPublished - Nov 2010

Fingerprint

Wetlands
Ammonium Compounds
Ammonia
vertical profile
Soil
ammonium
ammonia
wetland
Nitrification
Groundwater
nitrification
gene
community structure
soil
Archaeal Genes
oxidation
groundwater
Bacterial Structures
redox potential
Bacterial Genes

Keywords

  • ammonia
  • archaea
  • bacteria
  • biodegradation, environmental
  • DNA, archaeal
  • DNA, bacterial
  • nitrification
  • oxidation-reduction
  • phylogeny
  • polymorphism, restriction fragment length
  • quaternary ammonium compounds
  • soil microbiology
  • soil pollutants
  • wetlands

Cite this

Ammonium supply rate influences archaeal and bacterial ammonia oxidizers in a wetland soil vertical profile. / Höfferle, Špela; Nicol, Graeme W; Pal, Levin; Hacin, Janez; Prosser, James I; Mandic-Mulec, Ines.

In: FEMS Microbiology Ecology, Vol. 74, No. 2, 11.2010, p. 302-315.

Research output: Contribution to journalArticle

Höfferle, Špela ; Nicol, Graeme W ; Pal, Levin ; Hacin, Janez ; Prosser, James I ; Mandic-Mulec, Ines. / Ammonium supply rate influences archaeal and bacterial ammonia oxidizers in a wetland soil vertical profile. In: FEMS Microbiology Ecology. 2010 ; Vol. 74, No. 2. pp. 302-315.
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N2 - Oxidation of ammonia, the first step in nitrification, is carried out in soil by bacterial and archaeal ammonia oxidizers and recent studies suggest possible selection for the latter in low-ammonium environments. In this study, we investigated the selection of ammonia-oxidizing archaea and bacteria in wetland soil vertical profiles at two sites differing in terms of the ammonium supply rate, but not significantly in terms of the groundwater level. One site received ammonium through decomposition of organic matter, while the second, polluted site received a greater supply, through constant leakage of an underground septic tank. Soil nitrification potential was significantly greater at the polluted site. Quantification of amoA genes demonstrated greater abundance of bacterial than archaeal amoA genes throughout the soil profile at the polluted site, whereas bacterial amoA genes at the unpolluted site were below the detection limit. At both sites, archaeal, but not the bacterial community structure was clearly stratified with depth, with regard to the soil redox potential imposed by groundwater level. However, depth-related changes in the archaeal community structure may also be associated with physiological functions other than ammonia oxidation.

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