An alkali-halotolerant cellulase from Bacillus flexus isolated from green seaweed Ulva lactuca

Nitin Trivedi, Vishal Gupta, Manoj Kumar, Puja Kumari, C. R.K. Reddy, B. Jha

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84 Citations (Scopus)

Abstract

An extracellular alkali-halotolerant cellulase from the strain Bacillus flexus NT isolated from Ulva lactuca was purified to homogeneity with a recovery of 25.03% and purity fold of 22.31. The molecular weight of the enzyme was about 97 kDa and the Vmax and Km was 370.17 U/ml/min and 6.18 mg/ml respectively. The optimum pH and temperature for enzyme activity was 10 and 45 °C respectively. The enzymatic hydrolysis of the CMC was confirmed with GPC and GC-MS analysis. The stabilized activity of the enzyme even at high pH of 9.0-12.0 and residual activity of about 70% at salt concentration (NaCl 15%) revealed for its alkali-halotolerance nature. The metal ions Cd 2+ and Li1+ were found as inducers while Cr2+, Co2+, Zn2+ and metal chelator EDTA have significantly inhibited the enzyme activity. Enzyme activity was insensitive to ethanol and isopropanol while partially inhibited by acetone, cyclohexane and benzene.

Original languageEnglish
Pages (from-to)891-897
Number of pages7
JournalCarbohydrate Polymers
Volume83
Issue number2
DOIs
Publication statusPublished - 10 Jan 2011

Keywords

  • Cellulase
  • Cellulose
  • Halo-alkali tolerance
  • Marine habitat

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