An analysis of the diversity of Haemophilus parainfluenzae in the adult human respiratory tract by genomic DNA fingerprinting

Gifford Kerr, Ken J. Forbes, Alison Williams, Thomas Hugh Pennington

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6 Citations (Scopus)

Abstract

A method for typing Haemophilus species is described, based on the analysis of genomic DNA from Haemophilus parainfluenzae. The DNA was extracted by a rapid method and digested with the restriction enzyme BamHI to provide a characteristic 'fingerprint'. The pattern of fragments in the ranges 1-1.6 kb, 1.6-2 kb and 2-3 kb were used to produce a numerical profile of each isolate. In total 97 isolates were examined; 88 from throat swab material isolated from the 15 members of a British Antarctic Survey base and 9 type strains. Seventy-two of the 88 antarctic isolates were H. parainfluenzae and were found to be very diverse, comprising 41 identifiable strains with up to 5 strains being isolated from a single throat swab sample. There was evidence for both carriage and transmission within the isolated community. The technique provided a highly discriminatory method for characterizing Haemophilus strains which is suitable for epidemiological studies.

Original languageEnglish
Pages (from-to)89-98
Number of pages10
JournalEpidemiology and Infection
Volume111
Issue number1
Publication statusPublished - Aug 1993

Keywords

  • polyacrylamide gel-electrophoresis
  • hemophilus-influenzae
  • escherichia-coli
  • clonal analysis
  • H-influenzae
  • infections
  • children
  • epidemiology
  • transmission
  • population

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