An analysis of the phosphorylation and activation of extracellular-signal-regulated protein kinase 5 (ERK5) by mitogen-activated protein kinase kinase 5 (MKK5) in vitro

Nimesh Mody, David G Campbell, Nick Morrice, Mark Peggie, Philip Cohen

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

MKK5 expressed as a glutathione S-transferase fusion protein in human embryonic kidney 293 cells activated full-length extracellular-signal-regulated protein kinase (ERK)5 (ERK5wt) as well as the isolated catalytic domain (ERK5cat) in vitro. Activation was accompanied by the phosphorylation of Thr(219) and Tyr(221), the former residue being phosphorylated preferentially. ERK5cat phosphorylated at Thr(219), but not Tyr(221), possessed 10% of the activity of the doubly phosphorylated protein towards myelin basic protein, whereas ERK5cat phosphorylated at Tyr(221) alone was much less active. Activated ERK5 phosphorylated itself at a number of residues, including Thr(28), Ser(421), Ser(433), Ser(496), Ser(731) and Thr(733). ERK5 phosphorylated at Thr(219), but not Tyr(221), phosphorylated itself at a similar rate to ERK5 phosphorylated at both Thr(219) and Tyr(221). Activated ERK5 also phosphorylated mitogen-activated protein kinase kinase 5 (MKK5) extensively at Ser(129), Ser(137), Ser(142) and Ser(149), which are located within the region in MKK5 that is thought to interact with ERK5.
Original languageEnglish
Pages (from-to)567-575
Number of pages9
JournalBiochemical Journal
Volume372
Issue number2
DOIs
Publication statusPublished - 1 Jun 2003

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MAP Kinase Kinase 5
Mitogen-Activated Protein Kinase 7
Activation Analysis
MAP Kinase Kinase Kinases
Phosphorylation
Protein Kinases
Chemical activation
Myelin Basic Protein
Glutathione Transferase
In Vitro Techniques
Catalytic Domain
Proteins
Fusion reactions
Kidney

Keywords

  • Catalytic Domain
  • Cells, Cultured
  • Enzyme Activation
  • Glutathione Transferase
  • Humans
  • Mitogen-Activated Protein Kinase 7
  • Mitogen-Activated Protein Kinase Kinases
  • Mitogen-Activated Protein Kinases
  • Mutagenesis, Site-Directed
  • Mutation
  • Peptide Fragments
  • Phosphorylation
  • Recombinant Fusion Proteins
  • Threonine
  • Trypsin
  • Tyrosine

Cite this

An analysis of the phosphorylation and activation of extracellular-signal-regulated protein kinase 5 (ERK5) by mitogen-activated protein kinase kinase 5 (MKK5) in vitro. / Mody, Nimesh; Campbell, David G; Morrice, Nick; Peggie, Mark; Cohen, Philip.

In: Biochemical Journal, Vol. 372, No. 2, 01.06.2003, p. 567-575.

Research output: Contribution to journalArticle

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