AN ANTISENSE OLIGODEOXYRIBONUCLEOTIDE TO M-CALPAIN MESSENGER-RNA INHIBITS MYOBLAST FUSION

Denis Pierre Balcerzak; S  POUSSARD; J J  BRUSTIS; N  ELAMRANI; M  SORIANO; P  COTTIN; A  DUCASTAING

AN ANTISENSE OLIGODEOXYRIBONUCLEOTIDE TO M-CALPAIN MESSENGER-RNA INHIBITS MYOBLAST FUSION

Denis Pierre Balcerzak, S POUSSARD, J J BRUSTIS, N ELAMRANI, M SORIANO, P COTTIN, A DUCASTAING

The Rowett Institute of Nutrition and Health

Research output: Contribution to journal › Article › peer-review

65 Citations (Scopus)

Abstract

Previous studies have led to the hypothesis of a possible role for m-calpain (EC 3.4.22.17) in myoblast fusion in culture in vitro. To support this hypothesis, an antisense strategy has been used with cultured primary rat myoblasts. Using an appropriate antisense oligodeoxyribonucleotide to m-calpain mRNA, an inhibition of myoblast fusion has been observed, the maximum being obtained when the cell culture was treated with 30 mu M of oligomer. Synthesis of m-calpain was decreased by 48% while high concentrations of antisense oligonucleotide do not significantly affect myoblast proliferation. The specificity of m-calpain intervention during fusion has also been confirmed using antisense oligonucleotides to mu-calpain and p94 mRNAs, respectively.

Original language	English
Pages (from-to)	2077-2082
Number of pages	6
Journal	Journal of Cell Science
Volume	108
Publication status	Published - May 1995

Keywords

M-CALPAIN
ANTISENSE OLIGODEOXYRIBONUCLEOTIDE
MYOBLAST CULTURE
FUSION
ACTIVATED NEUTRAL PROTEASE
MOLECULAR-CLONING
SKELETAL-MUSCLE
MESSENGER-RNAS
C-MYB
CELLS
EXPRESSION
DIFFERENTIATION
CLEAVAGE
SYSTEM

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title = "AN ANTISENSE OLIGODEOXYRIBONUCLEOTIDE TO M-CALPAIN MESSENGER-RNA INHIBITS MYOBLAST FUSION",

abstract = "Previous studies have led to the hypothesis of a possible role for m-calpain (EC 3.4.22.17) in myoblast fusion in culture in vitro. To support this hypothesis, an antisense strategy has been used with cultured primary rat myoblasts. Using an appropriate antisense oligodeoxyribonucleotide to m-calpain mRNA, an inhibition of myoblast fusion has been observed, the maximum being obtained when the cell culture was treated with 30 mu M of oligomer. Synthesis of m-calpain was decreased by 48% while high concentrations of antisense oligonucleotide do not significantly affect myoblast proliferation. The specificity of m-calpain intervention during fusion has also been confirmed using antisense oligonucleotides to mu-calpain and p94 mRNAs, respectively.",

keywords = "M-CALPAIN, ANTISENSE OLIGODEOXYRIBONUCLEOTIDE, MYOBLAST CULTURE, FUSION, ACTIVATED NEUTRAL PROTEASE, MOLECULAR-CLONING, SKELETAL-MUSCLE, MESSENGER-RNAS, C-MYB, CELLS, EXPRESSION, DIFFERENTIATION, CLEAVAGE, SYSTEM",

author = "Balcerzak, {Denis Pierre} and S POUSSARD and BRUSTIS, {J J} and N ELAMRANI and M SORIANO and P COTTIN and A DUCASTAING",

year = "1995",

month = may,

language = "English",

volume = "108",

pages = "2077--2082",

journal = "Journal of Cell Science",

issn = "0021-9533",

publisher = "Company of Biologists Ltd",

}

TY - JOUR

T1 - AN ANTISENSE OLIGODEOXYRIBONUCLEOTIDE TO M-CALPAIN MESSENGER-RNA INHIBITS MYOBLAST FUSION

AU - Balcerzak, Denis Pierre

AU - POUSSARD, S

AU - BRUSTIS, J J

AU - ELAMRANI, N

AU - SORIANO, M

AU - COTTIN, P

AU - DUCASTAING, A

PY - 1995/5

Y1 - 1995/5

N2 - Previous studies have led to the hypothesis of a possible role for m-calpain (EC 3.4.22.17) in myoblast fusion in culture in vitro. To support this hypothesis, an antisense strategy has been used with cultured primary rat myoblasts. Using an appropriate antisense oligodeoxyribonucleotide to m-calpain mRNA, an inhibition of myoblast fusion has been observed, the maximum being obtained when the cell culture was treated with 30 mu M of oligomer. Synthesis of m-calpain was decreased by 48% while high concentrations of antisense oligonucleotide do not significantly affect myoblast proliferation. The specificity of m-calpain intervention during fusion has also been confirmed using antisense oligonucleotides to mu-calpain and p94 mRNAs, respectively.

AB - Previous studies have led to the hypothesis of a possible role for m-calpain (EC 3.4.22.17) in myoblast fusion in culture in vitro. To support this hypothesis, an antisense strategy has been used with cultured primary rat myoblasts. Using an appropriate antisense oligodeoxyribonucleotide to m-calpain mRNA, an inhibition of myoblast fusion has been observed, the maximum being obtained when the cell culture was treated with 30 mu M of oligomer. Synthesis of m-calpain was decreased by 48% while high concentrations of antisense oligonucleotide do not significantly affect myoblast proliferation. The specificity of m-calpain intervention during fusion has also been confirmed using antisense oligonucleotides to mu-calpain and p94 mRNAs, respectively.

KW - M-CALPAIN

KW - ANTISENSE OLIGODEOXYRIBONUCLEOTIDE

KW - MYOBLAST CULTURE

KW - FUSION

KW - ACTIVATED NEUTRAL PROTEASE

KW - MOLECULAR-CLONING

KW - SKELETAL-MUSCLE

KW - MESSENGER-RNAS

KW - C-MYB

KW - CELLS

KW - EXPRESSION

KW - DIFFERENTIATION

KW - CLEAVAGE

KW - SYSTEM

M3 - Article

SN - 0021-9533

VL - 108

SP - 2077

EP - 2082

JO - Journal of Cell Science

JF - Journal of Cell Science

ER -

AN ANTISENSE OLIGODEOXYRIBONUCLEOTIDE TO M-CALPAIN MESSENGER-RNA INHIBITS MYOBLAST FUSION

Abstract

Keywords

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