AN ANTISENSE OLIGODEOXYRIBONUCLEOTIDE TO M-CALPAIN MESSENGER-RNA INHIBITS MYOBLAST FUSION

Denis Pierre Balcerzak, S POUSSARD, J J BRUSTIS, N ELAMRANI, M SORIANO, P COTTIN, A DUCASTAING

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    Abstract

    Previous studies have led to the hypothesis of a possible role for m-calpain (EC 3.4.22.17) in myoblast fusion in culture in vitro. To support this hypothesis, an antisense strategy has been used with cultured primary rat myoblasts. Using an appropriate antisense oligodeoxyribonucleotide to m-calpain mRNA, an inhibition of myoblast fusion has been observed, the maximum being obtained when the cell culture was treated with 30 mu M of oligomer. Synthesis of m-calpain was decreased by 48% while high concentrations of antisense oligonucleotide do not significantly affect myoblast proliferation. The specificity of m-calpain intervention during fusion has also been confirmed using antisense oligonucleotides to mu-calpain and p94 mRNAs, respectively.

    Original languageEnglish
    Pages (from-to)2077-2082
    Number of pages6
    JournalJournal of Cell Science
    Volume108
    Publication statusPublished - May 1995

    Keywords

    • M-CALPAIN
    • ANTISENSE OLIGODEOXYRIBONUCLEOTIDE
    • MYOBLAST CULTURE
    • FUSION
    • ACTIVATED NEUTRAL PROTEASE
    • MOLECULAR-CLONING
    • SKELETAL-MUSCLE
    • MESSENGER-RNAS
    • C-MYB
    • CELLS
    • EXPRESSION
    • DIFFERENTIATION
    • CLEAVAGE
    • SYSTEM

    Cite this

    Balcerzak, D. P., POUSSARD, S., BRUSTIS, J. J., ELAMRANI, N., SORIANO, M., COTTIN, P., & DUCASTAING, A. (1995). AN ANTISENSE OLIGODEOXYRIBONUCLEOTIDE TO M-CALPAIN MESSENGER-RNA INHIBITS MYOBLAST FUSION. Journal of Cell Science, 108, 2077-2082.