AN ANTISENSE OLIGODEOXYRIBONUCLEOTIDE TO M-CALPAIN MESSENGER-RNA INHIBITS MYOBLAST FUSION

Denis Pierre Balcerzak, S POUSSARD, J J BRUSTIS, N ELAMRANI, M SORIANO, P COTTIN, A DUCASTAING

    Research output: Contribution to journalArticle

    64 Citations (Scopus)

    Abstract

    Previous studies have led to the hypothesis of a possible role for m-calpain (EC 3.4.22.17) in myoblast fusion in culture in vitro. To support this hypothesis, an antisense strategy has been used with cultured primary rat myoblasts. Using an appropriate antisense oligodeoxyribonucleotide to m-calpain mRNA, an inhibition of myoblast fusion has been observed, the maximum being obtained when the cell culture was treated with 30 mu M of oligomer. Synthesis of m-calpain was decreased by 48% while high concentrations of antisense oligonucleotide do not significantly affect myoblast proliferation. The specificity of m-calpain intervention during fusion has also been confirmed using antisense oligonucleotides to mu-calpain and p94 mRNAs, respectively.

    Original languageEnglish
    Pages (from-to)2077-2082
    Number of pages6
    JournalJournal of Cell Science
    Volume108
    Publication statusPublished - May 1995

    Keywords

    • M-CALPAIN
    • ANTISENSE OLIGODEOXYRIBONUCLEOTIDE
    • MYOBLAST CULTURE
    • FUSION
    • ACTIVATED NEUTRAL PROTEASE
    • MOLECULAR-CLONING
    • SKELETAL-MUSCLE
    • MESSENGER-RNAS
    • C-MYB
    • CELLS
    • EXPRESSION
    • DIFFERENTIATION
    • CLEAVAGE
    • SYSTEM

    Cite this

    Balcerzak, D. P., POUSSARD, S., BRUSTIS, J. J., ELAMRANI, N., SORIANO, M., COTTIN, P., & DUCASTAING, A. (1995). AN ANTISENSE OLIGODEOXYRIBONUCLEOTIDE TO M-CALPAIN MESSENGER-RNA INHIBITS MYOBLAST FUSION. Journal of Cell Science, 108, 2077-2082.

    AN ANTISENSE OLIGODEOXYRIBONUCLEOTIDE TO M-CALPAIN MESSENGER-RNA INHIBITS MYOBLAST FUSION. / Balcerzak, Denis Pierre; POUSSARD, S ; BRUSTIS, J J ; ELAMRANI, N ; SORIANO, M ; COTTIN, P ; DUCASTAING, A .

    In: Journal of Cell Science, Vol. 108, 05.1995, p. 2077-2082.

    Research output: Contribution to journalArticle

    Balcerzak, DP, POUSSARD, S, BRUSTIS, JJ, ELAMRANI, N, SORIANO, M, COTTIN, P & DUCASTAING, A 1995, 'AN ANTISENSE OLIGODEOXYRIBONUCLEOTIDE TO M-CALPAIN MESSENGER-RNA INHIBITS MYOBLAST FUSION' Journal of Cell Science, vol. 108, pp. 2077-2082.
    Balcerzak DP, POUSSARD S, BRUSTIS JJ, ELAMRANI N, SORIANO M, COTTIN P et al. AN ANTISENSE OLIGODEOXYRIBONUCLEOTIDE TO M-CALPAIN MESSENGER-RNA INHIBITS MYOBLAST FUSION. Journal of Cell Science. 1995 May;108:2077-2082.
    Balcerzak, Denis Pierre ; POUSSARD, S ; BRUSTIS, J J ; ELAMRANI, N ; SORIANO, M ; COTTIN, P ; DUCASTAING, A . / AN ANTISENSE OLIGODEOXYRIBONUCLEOTIDE TO M-CALPAIN MESSENGER-RNA INHIBITS MYOBLAST FUSION. In: Journal of Cell Science. 1995 ; Vol. 108. pp. 2077-2082.
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    abstract = "Previous studies have led to the hypothesis of a possible role for m-calpain (EC 3.4.22.17) in myoblast fusion in culture in vitro. To support this hypothesis, an antisense strategy has been used with cultured primary rat myoblasts. Using an appropriate antisense oligodeoxyribonucleotide to m-calpain mRNA, an inhibition of myoblast fusion has been observed, the maximum being obtained when the cell culture was treated with 30 mu M of oligomer. Synthesis of m-calpain was decreased by 48{\%} while high concentrations of antisense oligonucleotide do not significantly affect myoblast proliferation. The specificity of m-calpain intervention during fusion has also been confirmed using antisense oligonucleotides to mu-calpain and p94 mRNAs, respectively.",
    keywords = "M-CALPAIN, ANTISENSE OLIGODEOXYRIBONUCLEOTIDE, MYOBLAST CULTURE, FUSION, ACTIVATED NEUTRAL PROTEASE, MOLECULAR-CLONING, SKELETAL-MUSCLE, MESSENGER-RNAS, C-MYB, CELLS, EXPRESSION, DIFFERENTIATION, CLEAVAGE, SYSTEM",
    author = "Balcerzak, {Denis Pierre} and S POUSSARD and BRUSTIS, {J J} and N ELAMRANI and M SORIANO and P COTTIN and A DUCASTAING",
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    T1 - AN ANTISENSE OLIGODEOXYRIBONUCLEOTIDE TO M-CALPAIN MESSENGER-RNA INHIBITS MYOBLAST FUSION

    AU - Balcerzak, Denis Pierre

    AU - POUSSARD, S

    AU - BRUSTIS, J J

    AU - ELAMRANI, N

    AU - SORIANO, M

    AU - COTTIN, P

    AU - DUCASTAING, A

    PY - 1995/5

    Y1 - 1995/5

    N2 - Previous studies have led to the hypothesis of a possible role for m-calpain (EC 3.4.22.17) in myoblast fusion in culture in vitro. To support this hypothesis, an antisense strategy has been used with cultured primary rat myoblasts. Using an appropriate antisense oligodeoxyribonucleotide to m-calpain mRNA, an inhibition of myoblast fusion has been observed, the maximum being obtained when the cell culture was treated with 30 mu M of oligomer. Synthesis of m-calpain was decreased by 48% while high concentrations of antisense oligonucleotide do not significantly affect myoblast proliferation. The specificity of m-calpain intervention during fusion has also been confirmed using antisense oligonucleotides to mu-calpain and p94 mRNAs, respectively.

    AB - Previous studies have led to the hypothesis of a possible role for m-calpain (EC 3.4.22.17) in myoblast fusion in culture in vitro. To support this hypothesis, an antisense strategy has been used with cultured primary rat myoblasts. Using an appropriate antisense oligodeoxyribonucleotide to m-calpain mRNA, an inhibition of myoblast fusion has been observed, the maximum being obtained when the cell culture was treated with 30 mu M of oligomer. Synthesis of m-calpain was decreased by 48% while high concentrations of antisense oligonucleotide do not significantly affect myoblast proliferation. The specificity of m-calpain intervention during fusion has also been confirmed using antisense oligonucleotides to mu-calpain and p94 mRNAs, respectively.

    KW - M-CALPAIN

    KW - ANTISENSE OLIGODEOXYRIBONUCLEOTIDE

    KW - MYOBLAST CULTURE

    KW - FUSION

    KW - ACTIVATED NEUTRAL PROTEASE

    KW - MOLECULAR-CLONING

    KW - SKELETAL-MUSCLE

    KW - MESSENGER-RNAS

    KW - C-MYB

    KW - CELLS

    KW - EXPRESSION

    KW - DIFFERENTIATION

    KW - CLEAVAGE

    KW - SYSTEM

    M3 - Article

    VL - 108

    SP - 2077

    EP - 2082

    JO - Journal of Cell Science

    JF - Journal of Cell Science

    SN - 0021-9533

    ER -