An atomic force microscopy method for the detection of binding forces between bacteria and a lipid bilayer containing higher order gangliosides

Elizabeth L Adams, Salvador Almagro-Moreno, E Fidelma Boyd

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We developed an atomic force microscopy (AFM) method to determine the binding forces between a model cell wall plasma membrane and Vibrio cholerae. V. cholerae cells were covalently attached to AFM probes and forces were determined against a lipid bilayer containing sialic acid (N-acetylneuraminic acid) molecules as well as several control surfaces.
Original languageEnglish
Pages (from-to)352-354
Number of pages3
JournalJournal of Microbiological Methods
Volume84
Issue number2
Early online date28 Dec 2010
DOIs
Publication statusPublished - Feb 2011

Fingerprint

Vibrio cholerae
Gangliosides
Atomic Force Microscopy
Lipid Bilayers
N-Acetylneuraminic Acid
Bacteria
Cell Wall
Cell Membrane

Keywords

  • Animals
  • Bacterial Adhesion
  • Cell Membrane
  • Cells, Cultured
  • Gangliosides
  • Host-Pathogen Interactions
  • Lipid Bilayers
  • Mammals
  • Microscopy, Atomic Force
  • Vibrio cholerae

Cite this

An atomic force microscopy method for the detection of binding forces between bacteria and a lipid bilayer containing higher order gangliosides. / Adams, Elizabeth L; Almagro-Moreno, Salvador; Boyd, E Fidelma.

In: Journal of Microbiological Methods, Vol. 84, No. 2, 02.2011, p. 352-354.

Research output: Contribution to journalArticle

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