An atomic force microscopy method for the detection of binding forces between bacteria and a lipid bilayer containing higher order gangliosides

Elizabeth L Adams; Salvador Almagro-Moreno; E Fidelma Boyd

doi:10.1016/j.mimet.2010.12.014

An atomic force microscopy method for the detection of binding forces between bacteria and a lipid bilayer containing higher order gangliosides

Elizabeth L Adams, Salvador Almagro-Moreno, E Fidelma Boyd

School of Medicine, Medical Sciences & Nutrition

University of Delaware

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

We developed an atomic force microscopy (AFM) method to determine the binding forces between a model cell wall plasma membrane and Vibrio cholerae. V. cholerae cells were covalently attached to AFM probes and forces were determined against a lipid bilayer containing sialic acid (N-acetylneuraminic acid) molecules as well as several control surfaces.

Original language	English
Pages (from-to)	352-354
Number of pages	3
Journal	Journal of Microbiological Methods
Volume	84
Issue number	2
Early online date	28 Dec 2010
DOIs	https://doi.org/10.1016/j.mimet.2010.12.014
Publication status	Published - Feb 2011

Keywords

Animals
Bacterial Adhesion
Cell Membrane
Cells, Cultured
Gangliosides
Host-Pathogen Interactions
Lipid Bilayers
Mammals
Microscopy, Atomic Force
Vibrio cholerae

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10.1016/j.mimet.2010.12.014

Cite this

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title = "An atomic force microscopy method for the detection of binding forces between bacteria and a lipid bilayer containing higher order gangliosides",

abstract = "We developed an atomic force microscopy (AFM) method to determine the binding forces between a model cell wall plasma membrane and Vibrio cholerae. V. cholerae cells were covalently attached to AFM probes and forces were determined against a lipid bilayer containing sialic acid (N-acetylneuraminic acid) molecules as well as several control surfaces.",

keywords = "Animals, Bacterial Adhesion, Cell Membrane, Cells, Cultured, Gangliosides, Host-Pathogen Interactions, Lipid Bilayers, Mammals, Microscopy, Atomic Force, Vibrio cholerae",

author = "Adams, {Elizabeth L} and Salvador Almagro-Moreno and Boyd, {E Fidelma}",

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volume = "84",

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AU - Adams, Elizabeth L

AU - Almagro-Moreno, Salvador

AU - Boyd, E Fidelma

PY - 2011/2

Y1 - 2011/2

N2 - We developed an atomic force microscopy (AFM) method to determine the binding forces between a model cell wall plasma membrane and Vibrio cholerae. V. cholerae cells were covalently attached to AFM probes and forces were determined against a lipid bilayer containing sialic acid (N-acetylneuraminic acid) molecules as well as several control surfaces.

AB - We developed an atomic force microscopy (AFM) method to determine the binding forces between a model cell wall plasma membrane and Vibrio cholerae. V. cholerae cells were covalently attached to AFM probes and forces were determined against a lipid bilayer containing sialic acid (N-acetylneuraminic acid) molecules as well as several control surfaces.

KW - Animals

KW - Bacterial Adhesion

KW - Cell Membrane

KW - Cells, Cultured

KW - Gangliosides

KW - Host-Pathogen Interactions

KW - Lipid Bilayers

KW - Mammals

KW - Microscopy, Atomic Force

KW - Vibrio cholerae

U2 - 10.1016/j.mimet.2010.12.014

DO - 10.1016/j.mimet.2010.12.014

M3 - Article

C2 - 21192989

VL - 84

SP - 352

EP - 354

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 2

ER -

An atomic force microscopy method for the detection of binding forces between bacteria and a lipid bilayer containing higher order gangliosides

Abstract

Keywords

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