An ELISA for the detection of Bacillus subtilis L-form bacteria confirms their symbiosis in strawberry

C M J Ferguson, N A Booth, E J Allan

Research output: Contribution to journalArticle

23 Citations (Scopus)


Aims: To develop an ELISA for the detection of antigens derived from stable Bacillus subtilis L-form bacteria and to detect these in plants injected with L-form bacteria.

Methods and Results: A sandwich ELISA was developed and its specificity was investigated using L-forms and cell-walled forms of B. subtilis, different Bacillus species and a range of bacteria isolated from glasshouse-grown strawberry plants. The detection limits of the ELISA were approximately 10(3) viable cells ml(-1) for L-forms compared with 10(7) viable cells ml(-1) for cell-walled forms. Results showed that L-forms survived and moved within strawberry tissues injected with L-form bacteria.

Conclusion: An ELISA that selectively detects B. subtilis L-form bacteria was developed and shown to confirm the presence of L-forms in plants.

Significance and Impact of the Study: This will be a valuable rapid method to further studies on L-form plant interactions.

Original languageEnglish
Pages (from-to)390-394
Number of pages5
JournalLetters in Applied Microbiology
Publication statusPublished - 2000



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