An extensive endoplasmic reticulum-localised glycoprotein family in trypanosomatids

Harriet Allison, Amanda J O'Reilly, Jeremy M Sternberg, Mark C Field

Research output: Contribution to journalArticle

5 Citations (Scopus)
3 Downloads (Pure)

Abstract

African trypanosomes are evolutionarily highly divergent parasitic protozoa, and as a consequence the vast majority of trypanosome membrane proteins remain uncharacterised in terms of location, trafficking or function. Here we describe a novel family of type I membrane proteins which we designate ‘invariant glycoproteins’ (IGPs). IGPs are trypanosome-restricted, with extensive, lineage-specific paralogous expansions in related taxa. In T. bruceithree IGP subfamilies, IGP34, IGP40 and IGP48 are recognised; all possess a putative C-type lectin ectodomain and are ER-localised, despite lacking a classical ER-retention motif. IGPs exhibit highest expression in stumpy stage cells, suggesting roles in developmental progression, but gene silencing in mammalian infective forms suggests that each IGP subfamily is also required for normal proliferation. Detailed analysis of the IGP48 subfamily indicates a role in maintaining ER morphology, while the ER lumenal domain is necessary and sufficient for formation of both oligomeric complexes and ER retention. IGP48 is detected by antibodies from T. b. rhodesiense infected humans. We propose that the IGPs represent a trypanosomatid-specific family of ER-localised glycoproteins, with potential contributions to life cycle progression and immunity, and utilise oligomerisation as an ER retention mechanism.
Original languageEnglish
Pages (from-to)325-345
Number of pages21
JournalMicrobial Cell
Volume1
Issue number10
DOIs
Publication statusPublished - Oct 2014

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Endoplasmic Reticulum
Glycoproteins
Trypanosomiasis
Membrane Proteins
C-Type Lectins
Developmental Genes
Gene Silencing
Life Cycle Stages
Immunity
Antibodies

Keywords

  • Trypanosoma brucei
  • protein sorting
  • exocytosis
  • variant surface glycoprotein
  • endoplasmic reticulum
  • evolution
  • trypanosome

Cite this

An extensive endoplasmic reticulum-localised glycoprotein family in trypanosomatids. / Allison, Harriet; O'Reilly, Amanda J; Sternberg, Jeremy M; Field, Mark C.

In: Microbial Cell, Vol. 1, No. 10, 10.2014, p. 325-345.

Research output: Contribution to journalArticle

Allison, Harriet ; O'Reilly, Amanda J ; Sternberg, Jeremy M ; Field, Mark C. / An extensive endoplasmic reticulum-localised glycoprotein family in trypanosomatids. In: Microbial Cell. 2014 ; Vol. 1, No. 10. pp. 325-345.
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