An extra virgin olive oil-rich diet intervention ameliorates the non-alcoholic steatohepatitis induced by a high-fat "Western type" diet in mice

Enrique Jurado-Ruiz; Lourdes M Varela; Amparo Luque; Genoveva Berná; Gladys Cahuana; Enrique Martinez-Force; Rocío Gallego-Durán; Bernat Soria; Baukje de Roos; Manuel Romero Gómez; Franz Martín

doi:10.1002/mnfr.201600549

An extra virgin olive oil-rich diet intervention ameliorates the non-alcoholic steatohepatitis induced by a high-fat "Western type" diet in mice

Enrique Jurado-Ruiz, Lourdes M Varela, Amparo Luque, Genoveva Berná, Gladys Cahuana, Enrique Martinez-Force, Rocío Gallego-Durán, Bernat Soria, Baukje de Roos, Manuel Romero Gómez, Franz Martín

The Rowett Institute of Nutrition and Health

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54 Citations (Scopus)

Abstract

SCOPE: We evaluated the protective effect of extra virgin olive oil (EVOO) in high-fat diets (HFDs) on the inflammatory response and liver damage in a non-alcoholic fatty liver disease (NAFLD) mouse model.

METHODS AND RESULTS: C57BL/6J mice fed a standard diet or a lard-based HFD (HFD-L) for 12 weeks to develop NAFLD. HFD fed mice were then divided into four groups and fed for 24 weeks with: HFD-L; HFD-EVOO; HFD-OL (phenolics-rich EVOO) and R (reversion; standard diet). HFD-L-induced metabolic disorders were alleviated by replacement of lard with EVOO. EVOO diets improved plasma lipid profile and reduced body weight (BW), plasma and epididymal fat INF-γ, IL-6 and leptin levels and macrophage infiltration. Moreover, NAFLD Activity (NAS) scores were reduced. The liver lipid composition showed an increase in MUFAs, especially oleic acid, and a decrease in saturated fatty acids (SFAs). Hepatic adiponutrin and Cd36 gene expression was up-regulated in the EVOO groups. Liver Ingenuity pathway analysis (IPA) revealed in EVOO groups regulation of proteins involved in lipid metabolism, small molecule biochemistry, gastrointestinal disease and liver regeneration.

CONCLUSION: Dietary EVOO could repair HFD-induced hepatic damage, possibly via an anti-inflammatory effect in adipose tissue and modifications in the liver lipid composition and signaling pathways.

Original language	English
Article number	1600549
Pages (from-to)	1-12
Number of pages	12
Journal	Molecular Nutrition & Food Research
Volume	61
Issue number	3
Early online date	13 Dec 2016
DOIs	https://doi.org/10.1002/mnfr.201600549
Publication status	Published - 1 Mar 2017

Bibliographical note

Acknowledgements
EJR, LMV, AL and GB laboratory work. LMV, RGD, EMF and BR data analysis and
critical revision. FM, BS and MRG study design. LM and FM wrote the manuscript. FM sourced funding. Authors marked with an asterisk shared co-first authorship. This study was supported by grants AGL2014-54585-R (Ministerio de Economía y Competitividad) and PAI-BIO311 (Junta de Andalucía). LMV is funded by Fondo Europeo de Desarrollo Regional (FEDER).
The authors have no conflict of interest

Keywords

Extra virgin olive oil (EVOO)
high-fat diet
inflammation
nonoalcholic fatty liver disease
Triolein

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Baukje de Roos
- School of Medicine, Medical Sciences & Nutrition, The Rowett Institute of Nutrition and Health - Personal Chair
Person: Academic

Cite this

Jurado-Ruiz, E., Varela, L. M., Luque, A., Berná, G., Cahuana, G., Martinez-Force, E., Gallego-Durán, R., Soria, B., de Roos, B., Gómez, M. R., & Martín, F. (2017). An extra virgin olive oil-rich diet intervention ameliorates the non-alcoholic steatohepatitis induced by a high-fat "Western type" diet in mice. Molecular Nutrition & Food Research, 61(3), 1-12. Article 1600549 . https://doi.org/10.1002/mnfr.201600549

Jurado-Ruiz, E, Varela, LM, Luque, A, Berná, G, Cahuana, G, Martinez-Force, E, Gallego-Durán, R, Soria, B, de Roos, B, Gómez, MR & Martín, F 2017, 'An extra virgin olive oil-rich diet intervention ameliorates the non-alcoholic steatohepatitis induced by a high-fat "Western type" diet in mice', Molecular Nutrition & Food Research, vol. 61, no. 3, 1600549 , pp. 1-12. https://doi.org/10.1002/mnfr.201600549

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abstract = "SCOPE: We evaluated the protective effect of extra virgin olive oil (EVOO) in high-fat diets (HFDs) on the inflammatory response and liver damage in a non-alcoholic fatty liver disease (NAFLD) mouse model.METHODS AND RESULTS: C57BL/6J mice fed a standard diet or a lard-based HFD (HFD-L) for 12 weeks to develop NAFLD. HFD fed mice were then divided into four groups and fed for 24 weeks with: HFD-L; HFD-EVOO; HFD-OL (phenolics-rich EVOO) and R (reversion; standard diet). HFD-L-induced metabolic disorders were alleviated by replacement of lard with EVOO. EVOO diets improved plasma lipid profile and reduced body weight (BW), plasma and epididymal fat INF-γ, IL-6 and leptin levels and macrophage infiltration. Moreover, NAFLD Activity (NAS) scores were reduced. The liver lipid composition showed an increase in MUFAs, especially oleic acid, and a decrease in saturated fatty acids (SFAs). Hepatic adiponutrin and Cd36 gene expression was up-regulated in the EVOO groups. Liver Ingenuity pathway analysis (IPA) revealed in EVOO groups regulation of proteins involved in lipid metabolism, small molecule biochemistry, gastrointestinal disease and liver regeneration.CONCLUSION: Dietary EVOO could repair HFD-induced hepatic damage, possibly via an anti-inflammatory effect in adipose tissue and modifications in the liver lipid composition and signaling pathways. ",

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note = "Acknowledgements EJR, LMV, AL and GB laboratory work. LMV, RGD, EMF and BR data analysis and critical revision. FM, BS and MRG study design. LM and FM wrote the manuscript. FM sourced funding. Authors marked with an asterisk shared co-first authorship. This study was supported by grants AGL2014-54585-R (Ministerio de Econom{\'i}a y Competitividad) and PAI-BIO311 (Junta de Andaluc{\'i}a). LMV is funded by Fondo Europeo de Desarrollo Regional (FEDER). The authors have no conflict of interest",

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T1 - An extra virgin olive oil-rich diet intervention ameliorates the non-alcoholic steatohepatitis induced by a high-fat "Western type" diet in mice

AU - Jurado-Ruiz, Enrique

AU - Varela, Lourdes M

AU - Luque, Amparo

AU - Berná, Genoveva

AU - Cahuana, Gladys

AU - Martinez-Force, Enrique

AU - Gallego-Durán, Rocío

AU - Soria, Bernat

AU - de Roos, Baukje

AU - Gómez, Manuel Romero

AU - Martín, Franz

N1 - Acknowledgements EJR, LMV, AL and GB laboratory work. LMV, RGD, EMF and BR data analysis and critical revision. FM, BS and MRG study design. LM and FM wrote the manuscript. FM sourced funding. Authors marked with an asterisk shared co-first authorship. This study was supported by grants AGL2014-54585-R (Ministerio de Economía y Competitividad) and PAI-BIO311 (Junta de Andalucía). LMV is funded by Fondo Europeo de Desarrollo Regional (FEDER). The authors have no conflict of interest

PY - 2017/3/1

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N2 - SCOPE: We evaluated the protective effect of extra virgin olive oil (EVOO) in high-fat diets (HFDs) on the inflammatory response and liver damage in a non-alcoholic fatty liver disease (NAFLD) mouse model.METHODS AND RESULTS: C57BL/6J mice fed a standard diet or a lard-based HFD (HFD-L) for 12 weeks to develop NAFLD. HFD fed mice were then divided into four groups and fed for 24 weeks with: HFD-L; HFD-EVOO; HFD-OL (phenolics-rich EVOO) and R (reversion; standard diet). HFD-L-induced metabolic disorders were alleviated by replacement of lard with EVOO. EVOO diets improved plasma lipid profile and reduced body weight (BW), plasma and epididymal fat INF-γ, IL-6 and leptin levels and macrophage infiltration. Moreover, NAFLD Activity (NAS) scores were reduced. The liver lipid composition showed an increase in MUFAs, especially oleic acid, and a decrease in saturated fatty acids (SFAs). Hepatic adiponutrin and Cd36 gene expression was up-regulated in the EVOO groups. Liver Ingenuity pathway analysis (IPA) revealed in EVOO groups regulation of proteins involved in lipid metabolism, small molecule biochemistry, gastrointestinal disease and liver regeneration.CONCLUSION: Dietary EVOO could repair HFD-induced hepatic damage, possibly via an anti-inflammatory effect in adipose tissue and modifications in the liver lipid composition and signaling pathways.

AB - SCOPE: We evaluated the protective effect of extra virgin olive oil (EVOO) in high-fat diets (HFDs) on the inflammatory response and liver damage in a non-alcoholic fatty liver disease (NAFLD) mouse model.METHODS AND RESULTS: C57BL/6J mice fed a standard diet or a lard-based HFD (HFD-L) for 12 weeks to develop NAFLD. HFD fed mice were then divided into four groups and fed for 24 weeks with: HFD-L; HFD-EVOO; HFD-OL (phenolics-rich EVOO) and R (reversion; standard diet). HFD-L-induced metabolic disorders were alleviated by replacement of lard with EVOO. EVOO diets improved plasma lipid profile and reduced body weight (BW), plasma and epididymal fat INF-γ, IL-6 and leptin levels and macrophage infiltration. Moreover, NAFLD Activity (NAS) scores were reduced. The liver lipid composition showed an increase in MUFAs, especially oleic acid, and a decrease in saturated fatty acids (SFAs). Hepatic adiponutrin and Cd36 gene expression was up-regulated in the EVOO groups. Liver Ingenuity pathway analysis (IPA) revealed in EVOO groups regulation of proteins involved in lipid metabolism, small molecule biochemistry, gastrointestinal disease and liver regeneration.CONCLUSION: Dietary EVOO could repair HFD-induced hepatic damage, possibly via an anti-inflammatory effect in adipose tissue and modifications in the liver lipid composition and signaling pathways.

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KW - high-fat diet

KW - inflammation

KW - nonoalcholic fatty liver disease

KW - Triolein

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DO - 10.1002/mnfr.201600549

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SN - 1613-4125

VL - 61

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JO - Molecular Nutrition & Food Research

JF - Molecular Nutrition & Food Research

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M1 - 1600549

ER -

An extra virgin olive oil-rich diet intervention ameliorates the non-alcoholic steatohepatitis induced by a high-fat "Western type" diet in mice

Abstract

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Keywords

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