An extra virgin olive oil-rich diet intervention ameliorates the non-alcoholic steatohepatitis induced by a high-fat "Western type" diet in mice

Enrique Jurado-Ruiz, Lourdes M Varela, Amparo Luque, Genoveva Berná, Gladys Cahuana, Enrique Martinez-Force, Rocío Gallego-Durán, Bernat Soria, Baukje de Roos, Manuel Romero Gómez, Franz Martín

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Abstract

SCOPE: We evaluated the protective effect of extra virgin olive oil (EVOO) in high-fat diets (HFDs) on the inflammatory response and liver damage in a non-alcoholic fatty liver disease (NAFLD) mouse model.

METHODS AND RESULTS: C57BL/6J mice fed a standard diet or a lard-based HFD (HFD-L) for 12 weeks to develop NAFLD. HFD fed mice were then divided into four groups and fed for 24 weeks with: HFD-L; HFD-EVOO; HFD-OL (phenolics-rich EVOO) and R (reversion; standard diet). HFD-L-induced metabolic disorders were alleviated by replacement of lard with EVOO. EVOO diets improved plasma lipid profile and reduced body weight (BW), plasma and epididymal fat INF-γ, IL-6 and leptin levels and macrophage infiltration. Moreover, NAFLD Activity (NAS) scores were reduced. The liver lipid composition showed an increase in MUFAs, especially oleic acid, and a decrease in saturated fatty acids (SFAs). Hepatic adiponutrin and Cd36 gene expression was up-regulated in the EVOO groups. Liver Ingenuity pathway analysis (IPA) revealed in EVOO groups regulation of proteins involved in lipid metabolism, small molecule biochemistry, gastrointestinal disease and liver regeneration.

CONCLUSION: Dietary EVOO could repair HFD-induced hepatic damage, possibly via an anti-inflammatory effect in adipose tissue and modifications in the liver lipid composition and signaling pathways.

Original languageEnglish
Article number1600549
Pages (from-to)1-12
Number of pages12
JournalMolecular Nutrition & Food Research
Volume61
Issue number3
Early online date13 Dec 2016
DOIs
Publication statusPublished - 1 Mar 2017

Keywords

  • Extra virgin olive oil (EVOO)
  • high-fat diet
  • inflammation
  • nonoalcholic fatty liver disease
  • Triolein

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    Jurado-Ruiz, E., Varela, L. M., Luque, A., Berná, G., Cahuana, G., Martinez-Force, E., Gallego-Durán, R., Soria, B., de Roos, B., Gómez, M. R., & Martín, F. (2017). An extra virgin olive oil-rich diet intervention ameliorates the non-alcoholic steatohepatitis induced by a high-fat "Western type" diet in mice. Molecular Nutrition & Food Research, 61(3), 1-12. [1600549 ]. https://doi.org/10.1002/mnfr.201600549