An extremely rapid and simple DNA-release method for detection of M. tuberculosis from clinical specimens

Lynn Marie Thomson; H. Traore; Hasan Yesilkaya; C. Doig; H. Steingrimsdottir; L. Garcia; Kenneth James Forbes

doi:10.1016/j.mimet.2005.03.005

An extremely rapid and simple DNA-release method for detection of M. tuberculosis from clinical specimens

Lynn Marie Thomson, H. Traore, Hasan Yesilkaya, C. Doig, H. Steingrimsdottir, L. Garcia, Kenneth James Forbes

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

A single-step, 5-min lysis method was investigated as a rapid technique to extract genomic DNA from mycobacteria for PCR detection of M. tuberculosis directly from clinical specimens. Of 67 smear-positive clinical specimens, 64 (95.5%) were positive by PCR after this rapid extraction method. (c) 2005 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	95-98
Number of pages	3
Journal	Journal of Microbiological Methods
Volume	63
Issue number	1
DOIs	https://doi.org/10.1016/j.mimet.2005.03.005
Publication status	Published - 2005

Keywords

mycobacterium
sputum
PCR
rapid
detection
POLYMERASE-CHAIN-REACTION
NUCLEIC-ACID AMPLIFICATION
MYCOBACTERIUM-TUBERCULOSIS
DIAGNOSIS
SAMPLES
ASSAY

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.mimet.2005.03.005

Cite this

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title = "An extremely rapid and simple DNA-release method for detection of M. tuberculosis from clinical specimens",

abstract = "A single-step, 5-min lysis method was investigated as a rapid technique to extract genomic DNA from mycobacteria for PCR detection of M. tuberculosis directly from clinical specimens. Of 67 smear-positive clinical specimens, 64 (95.5%) were positive by PCR after this rapid extraction method. (c) 2005 Elsevier B.V. All rights reserved.",

keywords = "mycobacterium, sputum, PCR, rapid, detection, POLYMERASE-CHAIN-REACTION, NUCLEIC-ACID AMPLIFICATION, MYCOBACTERIUM-TUBERCULOSIS, DIAGNOSIS, SAMPLES, ASSAY",

author = "Thomson, {Lynn Marie} and H. Traore and Hasan Yesilkaya and C. Doig and H. Steingrimsdottir and L. Garcia and Forbes, {Kenneth James}",

year = "2005",

doi = "10.1016/j.mimet.2005.03.005",

language = "English",

volume = "63",

pages = "95--98",

journal = "Journal of Microbiological Methods",

issn = "0167-7012",

publisher = "Elsevier",

number = "1",

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TY - JOUR

T1 - An extremely rapid and simple DNA-release method for detection of M. tuberculosis from clinical specimens

AU - Thomson, Lynn Marie

AU - Traore, H.

AU - Yesilkaya, Hasan

AU - Doig, C.

AU - Steingrimsdottir, H.

AU - Garcia, L.

AU - Forbes, Kenneth James

PY - 2005

Y1 - 2005

N2 - A single-step, 5-min lysis method was investigated as a rapid technique to extract genomic DNA from mycobacteria for PCR detection of M. tuberculosis directly from clinical specimens. Of 67 smear-positive clinical specimens, 64 (95.5%) were positive by PCR after this rapid extraction method. (c) 2005 Elsevier B.V. All rights reserved.

AB - A single-step, 5-min lysis method was investigated as a rapid technique to extract genomic DNA from mycobacteria for PCR detection of M. tuberculosis directly from clinical specimens. Of 67 smear-positive clinical specimens, 64 (95.5%) were positive by PCR after this rapid extraction method. (c) 2005 Elsevier B.V. All rights reserved.

KW - mycobacterium

KW - sputum

KW - PCR

KW - rapid

KW - detection

KW - POLYMERASE-CHAIN-REACTION

KW - NUCLEIC-ACID AMPLIFICATION

KW - MYCOBACTERIUM-TUBERCULOSIS

KW - DIAGNOSIS

KW - SAMPLES

KW - ASSAY

U2 - 10.1016/j.mimet.2005.03.005

DO - 10.1016/j.mimet.2005.03.005

M3 - Article

SN - 0167-7012

VL - 63

SP - 95

EP - 98

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 1

ER -

An extremely rapid and simple DNA-release method for detection of M. tuberculosis from clinical specimens

Abstract

Keywords

UN SDGs

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