An extremely rapid and simple DNA-release method for detection of M. tuberculosis from clinical specimens

Lynn Marie Thomson, H. Traore, Hasan Yesilkaya, C. Doig, H. Steingrimsdottir, L. Garcia, Kenneth James Forbes

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13 Citations (Scopus)

Abstract

A single-step, 5-min lysis method was investigated as a rapid technique to extract genomic DNA from mycobacteria for PCR detection of M. tuberculosis directly from clinical specimens. Of 67 smear-positive clinical specimens, 64 (95.5%) were positive by PCR after this rapid extraction method. (c) 2005 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)95-98
Number of pages3
JournalJournal of Microbiological Methods
Volume63
Issue number1
DOIs
Publication statusPublished - 2005

Keywords

  • mycobacterium
  • sputum
  • PCR
  • rapid
  • detection
  • POLYMERASE-CHAIN-REACTION
  • NUCLEIC-ACID AMPLIFICATION
  • MYCOBACTERIUM-TUBERCULOSIS
  • DIAGNOSIS
  • SAMPLES
  • ASSAY

Cite this

Thomson, L. M., Traore, H., Yesilkaya, H., Doig, C., Steingrimsdottir, H., Garcia, L., & Forbes, K. J. (2005). An extremely rapid and simple DNA-release method for detection of M. tuberculosis from clinical specimens. Journal of Microbiological Methods, 63(1), 95-98. https://doi.org/10.1016/j.mimet.2005.03.005