An Immunomagnetic Separator for Concentration of Pathogenic Microorganisms from Large Volume Samples

Ovidiu Rotariu, Iain D Ogden, Marion MacRae, V. Badescu, N. J. C. Strachan

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The standard method of immunomagnetic separation of pathogenic bacteria from food and environmental matrices processes 1 ml volumes. Pathogens present at low levels (< 1 pathogenic bacteria per ml) will not be consistently detected by this method. Here a flow through immunomagnetic separator (FTIMS) has been designed and tested to process large volume samples (> 50ml). Preliminary results show that between 70 and 113 times more Escherchia coli O157 are recovered compared with the standard 1 ml method. (c) 2005 Published by Elsevier B.V.

Original languageEnglish
Pages (from-to)589-596
Number of pages7
JournalJournal of Magnetism and Magnetic Materials
Volume293
DOIs
Publication statusPublished - 2005

Keywords

  • immunomagnetic separation
  • magnetic particles
  • pathogenic micro-organisms
  • E. coli O157
  • mathematical modeling
  • food pathogens
  • ESCHERICHIA-COLI O157
  • BOUNDED FLOW-FIELD
  • FILTER
  • CELL

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