An Immunomagnetic Separator for Concentration of Pathogenic Microorganisms from Large Volume Samples

Ovidiu Rotariu; Iain D Ogden; Marion MacRae; V. Badescu; N. J. C. Strachan

doi:10.1016/j.jmmm.2005.01.078

An Immunomagnetic Separator for Concentration of Pathogenic Microorganisms from Large Volume Samples

Ovidiu Rotariu, Iain D Ogden, Marion MacRae, V. Badescu, N. J. C. Strachan

Applied Medicine

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

The standard method of immunomagnetic separation of pathogenic bacteria from food and environmental matrices processes 1 ml volumes. Pathogens present at low levels (< 1 pathogenic bacteria per ml) will not be consistently detected by this method. Here a flow through immunomagnetic separator (FTIMS) has been designed and tested to process large volume samples (> 50ml). Preliminary results show that between 70 and 113 times more Escherchia coli O157 are recovered compared with the standard 1 ml method. (c) 2005 Published by Elsevier B.V.

Original language	English
Pages (from-to)	589-596
Number of pages	7
Journal	Journal of Magnetism and Magnetic Materials
Volume	293
DOIs	https://doi.org/10.1016/j.jmmm.2005.01.078
Publication status	Published - 2005

Keywords

immunomagnetic separation
magnetic particles
pathogenic micro-organisms
E. coli O157
mathematical modeling
food pathogens
ESCHERICHIA-COLI O157
BOUNDED FLOW-FIELD
FILTER
CELL

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title = "An Immunomagnetic Separator for Concentration of Pathogenic Microorganisms from Large Volume Samples",

abstract = "The standard method of immunomagnetic separation of pathogenic bacteria from food and environmental matrices processes 1 ml volumes. Pathogens present at low levels (< 1 pathogenic bacteria per ml) will not be consistently detected by this method. Here a flow through immunomagnetic separator (FTIMS) has been designed and tested to process large volume samples (> 50ml). Preliminary results show that between 70 and 113 times more Escherchia coli O157 are recovered compared with the standard 1 ml method. (c) 2005 Published by Elsevier B.V.",

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language = "English",

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journal = "Journal of Magnetism and Magnetic Materials",

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T1 - An Immunomagnetic Separator for Concentration of Pathogenic Microorganisms from Large Volume Samples

AU - Rotariu, Ovidiu

AU - Ogden, Iain D

AU - MacRae, Marion

AU - Badescu, V.

AU - Strachan, N. J. C.

PY - 2005

Y1 - 2005

N2 - The standard method of immunomagnetic separation of pathogenic bacteria from food and environmental matrices processes 1 ml volumes. Pathogens present at low levels (< 1 pathogenic bacteria per ml) will not be consistently detected by this method. Here a flow through immunomagnetic separator (FTIMS) has been designed and tested to process large volume samples (> 50ml). Preliminary results show that between 70 and 113 times more Escherchia coli O157 are recovered compared with the standard 1 ml method. (c) 2005 Published by Elsevier B.V.

AB - The standard method of immunomagnetic separation of pathogenic bacteria from food and environmental matrices processes 1 ml volumes. Pathogens present at low levels (< 1 pathogenic bacteria per ml) will not be consistently detected by this method. Here a flow through immunomagnetic separator (FTIMS) has been designed and tested to process large volume samples (> 50ml). Preliminary results show that between 70 and 113 times more Escherchia coli O157 are recovered compared with the standard 1 ml method. (c) 2005 Published by Elsevier B.V.

KW - immunomagnetic separation

KW - magnetic particles

KW - pathogenic micro-organisms

KW - E. coli O157

KW - mathematical modeling

KW - food pathogens

KW - ESCHERICHIA-COLI O157

KW - BOUNDED FLOW-FIELD

KW - FILTER

KW - CELL

U2 - 10.1016/j.jmmm.2005.01.078

DO - 10.1016/j.jmmm.2005.01.078

M3 - Article

SN - 0304-8853

VL - 293

SP - 589

EP - 596

JO - Journal of Magnetism and Magnetic Materials

JF - Journal of Magnetism and Magnetic Materials

ER -

An Immunomagnetic Separator for Concentration of Pathogenic Microorganisms from Large Volume Samples

Abstract

Keywords

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