An internal polarity landmark is important for externally induced hyphal behaviors in Candida albicans

Alexandra Carolyn Brand, Anjalee Vacharaksa, Catherine Bendel, J Norton, Paula Haynes, Michelle Henry-Stanley, Colin Wells, Karen Ross, Neil Andrew Robert Gow, Cheryl A Gale

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Directional growth is a function of polarized cells such as neurites, pollen tubes, and fungal hyphae. Correct orientation of the extending cell tip depends on signaling pathways and effectors that mediate asymmetric responses to specific environmental cues. In the hyphal form of the eukaryotic fungal pathogen Candida albicans, these responses include thigmotropism and galvanotropism (hyphal turning in response to changes in substrate topography and imposed electrical fields, respectively) and penetration into semisolid substrates. During vegetative growth in C. albicans, as in the model yeast Saccharomyces cerevisiae, the Ras-like GTPase Rsr1 mediates internal cellular cues to position new buds in a prespecified pattern on the mother cell cortex. Here, we demonstrate that Rsr1 is also important for hyphal tip orientation in response to the external environmental cues that induce thigmotropic and galvanotropic growth. In addition, Rsr1 is involved in hyphal interactions with epithelial cells in vitro and its deletion diminishes the hyphal invasion of kidney tissue during systemic infection. Thus, Rsr1, an internal polarity landmark in yeast, is also involved in polarized growth responses to asymmetric environmental signals, a paradigm that is different from that described for the homologous protein in S. cerevisiae. Rsr1 may thereby contribute to the pathogenesis of C. albicans infections by influencing hyphal tip responses triggered by interaction with host tissues.
Original languageEnglish
Pages (from-to)712-720
Number of pages9
JournalEukaryotic Cell
Volume7
Issue number4
DOIs
Publication statusPublished - Apr 2008

Fingerprint

Candida albicans
Cues
Growth
Yeasts
Pollen Tube
Saccharomyces cerevisiae Proteins
ras Proteins
Hyphae
Neurites
Infection
Saccharomyces cerevisiae
Stem Cells
Epithelial Cells
Kidney

Keywords

  • Animals
  • Candida albicans
  • Candidiasis
  • Cell Polarity
  • Epithelial Cells
  • Female
  • Fungal Proteins
  • GTP Phosphohydrolases
  • Hyphae
  • Kidney
  • Mice
  • Mouth
  • rab GTP-Binding Proteins

Cite this

Brand, A. C., Vacharaksa, A., Bendel, C., Norton, J., Haynes, P., Henry-Stanley, M., ... Gale, C. A. (2008). An internal polarity landmark is important for externally induced hyphal behaviors in Candida albicans. Eukaryotic Cell, 7(4), 712-720. https://doi.org/10.1128/EC.00453-07

An internal polarity landmark is important for externally induced hyphal behaviors in Candida albicans. / Brand, Alexandra Carolyn; Vacharaksa, Anjalee; Bendel, Catherine; Norton, J; Haynes, Paula; Henry-Stanley, Michelle; Wells, Colin; Ross, Karen; Gow, Neil Andrew Robert; Gale, Cheryl A.

In: Eukaryotic Cell, Vol. 7, No. 4, 04.2008, p. 712-720.

Research output: Contribution to journalArticle

Brand, AC, Vacharaksa, A, Bendel, C, Norton, J, Haynes, P, Henry-Stanley, M, Wells, C, Ross, K, Gow, NAR & Gale, CA 2008, 'An internal polarity landmark is important for externally induced hyphal behaviors in Candida albicans', Eukaryotic Cell, vol. 7, no. 4, pp. 712-720. https://doi.org/10.1128/EC.00453-07
Brand AC, Vacharaksa A, Bendel C, Norton J, Haynes P, Henry-Stanley M et al. An internal polarity landmark is important for externally induced hyphal behaviors in Candida albicans. Eukaryotic Cell. 2008 Apr;7(4):712-720. https://doi.org/10.1128/EC.00453-07
Brand, Alexandra Carolyn ; Vacharaksa, Anjalee ; Bendel, Catherine ; Norton, J ; Haynes, Paula ; Henry-Stanley, Michelle ; Wells, Colin ; Ross, Karen ; Gow, Neil Andrew Robert ; Gale, Cheryl A. / An internal polarity landmark is important for externally induced hyphal behaviors in Candida albicans. In: Eukaryotic Cell. 2008 ; Vol. 7, No. 4. pp. 712-720.
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abstract = "Directional growth is a function of polarized cells such as neurites, pollen tubes, and fungal hyphae. Correct orientation of the extending cell tip depends on signaling pathways and effectors that mediate asymmetric responses to specific environmental cues. In the hyphal form of the eukaryotic fungal pathogen Candida albicans, these responses include thigmotropism and galvanotropism (hyphal turning in response to changes in substrate topography and imposed electrical fields, respectively) and penetration into semisolid substrates. During vegetative growth in C. albicans, as in the model yeast Saccharomyces cerevisiae, the Ras-like GTPase Rsr1 mediates internal cellular cues to position new buds in a prespecified pattern on the mother cell cortex. Here, we demonstrate that Rsr1 is also important for hyphal tip orientation in response to the external environmental cues that induce thigmotropic and galvanotropic growth. In addition, Rsr1 is involved in hyphal interactions with epithelial cells in vitro and its deletion diminishes the hyphal invasion of kidney tissue during systemic infection. Thus, Rsr1, an internal polarity landmark in yeast, is also involved in polarized growth responses to asymmetric environmental signals, a paradigm that is different from that described for the homologous protein in S. cerevisiae. Rsr1 may thereby contribute to the pathogenesis of C. albicans infections by influencing hyphal tip responses triggered by interaction with host tissues.",
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