An investigation of the effects of endometriosis on the proteome of human eutopic endometrium: A heterogeneous tissue with a complex disease

Paul Alfred Francois Fowler, Jenny Tattum, Siladitya Bhattacharya, Thomas Klonisch, Sabine Hombach-Klonisch, Rafet Gazvani, Richard G. Lea, Iain D Miller, William Simpson, Phillip Cash

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

The pathogenesis of endometriosis includes the proliferation of heterogeneous endometrial cells and their invasion into ectopic sites within the peritoneal cavity. This may be due to abnormalities of the eutopic endometrium itself, predisposing the cells to survive and implant ectopically. We investigated the applicability of 2-DE gels and peptide mass mapping to identify candidate endometrial proteins with a role in endometriosis. Despite the heterogeneous nature of endometrium, our results show that combining the analysis of 2-DE gels and peptide mass mapping yields consistent data. We identified dysregulated proteins in women with endometriosis which included: (i) molecular chaperones including heat shock protein 90 and annexin A2, (ii) proteins involved in cellular redox state, such as peroxiredoxin 2, (iii) proteins involved in protein and DNA formation/breakdown, including ribonucleoside-diphosphate reductase, prohibitin and prolyl 4-hydroxylase, and (iv) secreted proteins, such as apolipoprotein A1. These proteins have functions which suggest that they could play a role in the pathogenesis of endometriosis. This study demonstrated that 2-DE gel analysis and mass spectroscopic protein identification are suitable for the identification of proteins with candidate associations with endometriosis. These techniques should be used on a larger scale to identify endometriosis-related proteins, thus improving the understanding of this complex disease.

Original languageEnglish
Pages (from-to)130-142
Number of pages13
JournalProteomics
Volume7
Issue number1
Early online date24 Nov 2006
DOIs
Publication statusPublished - Jan 2007

Keywords

  • endometriosis
  • eutopic
  • human
  • menstrual cycle
  • 2-dimensional gel analysis
  • DNA microarray analysis
  • normal menstrual-cycle
  • heat-shock proteins
  • gene-expression
  • in-vitro
  • cell-proliferation
  • peritoneal-fluid
  • oxidative stress
  • stromal cells

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