An observational prospective study of topical acidified nitrite for killing methicillin-resistant Staphylococcus aureus (MRSA) in contaminated wounds

Anthony D Ormerod, Amjad A J Shah, Hong Li, Nigel B Benjamin, Gail P Ferguson, Carlo Leifert

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3 Downloads (Pure)

Abstract

Background
Endogenous nitric oxide (NO) kills bacteria and other organisms as part of the innate immune response. When nitrite is exposed to low pH, NO is generated and has been used as an NO delivery system to treat skin infections. We demonstrated eradication of MRSA carriage from wounds using a topical formulation of citric acid (4.5%) and sodium nitrite (3%) creams co-applied for 5 days to 15 wounds in an observational prospective pilot study of 8 patients.

Findings
Following treatment with topical citric acid and sodium nitrite, 9 of 15 wounds (60%) and 3 of 8 patients (37%) were cleared of infection. MRSA isolates from these patients were all sensitive to acidified nitrite in vitro compared to methicillin-sensitive S. aureus and a reference strain of MRSA.

Conclusions
Nitric oxide and acidified nitrite offer a novel therapy for control of MRSA in wounds. Wounds that were not cleared of infection may have been re-contaminated or the bioavailability of acidified nitrite impaired by local factors in the tissue.
Original languageEnglish
Article number458
Number of pages7
JournalBMC Research Notes
Volume4
DOIs
Publication statusPublished - 27 Oct 2011

Fingerprint

Methicillin
Methicillin-Resistant Staphylococcus aureus
Nitrites
Observational Studies
Prospective Studies
Wounds and Injuries
Sodium Nitrite
Nitric Oxide
Citric Acid
Infection
Thromboplastin
Innate Immunity
Oxides
Biological Availability
Staphylococcus aureus
Bacteria
Skin
Tissue
Therapeutics

Cite this

An observational prospective study of topical acidified nitrite for killing methicillin-resistant Staphylococcus aureus (MRSA) in contaminated wounds. / Ormerod, Anthony D; Shah, Amjad A J; Li, Hong; Benjamin, Nigel B; Ferguson, Gail P; Leifert, Carlo.

In: BMC Research Notes, Vol. 4, 458, 27.10.2011.

Research output: Contribution to journalArticle

Ormerod, Anthony D ; Shah, Amjad A J ; Li, Hong ; Benjamin, Nigel B ; Ferguson, Gail P ; Leifert, Carlo. / An observational prospective study of topical acidified nitrite for killing methicillin-resistant Staphylococcus aureus (MRSA) in contaminated wounds. In: BMC Research Notes. 2011 ; Vol. 4.
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abstract = "BackgroundEndogenous nitric oxide (NO) kills bacteria and other organisms as part of the innate immune response. When nitrite is exposed to low pH, NO is generated and has been used as an NO delivery system to treat skin infections. We demonstrated eradication of MRSA carriage from wounds using a topical formulation of citric acid (4.5{\%}) and sodium nitrite (3{\%}) creams co-applied for 5 days to 15 wounds in an observational prospective pilot study of 8 patients. FindingsFollowing treatment with topical citric acid and sodium nitrite, 9 of 15 wounds (60{\%}) and 3 of 8 patients (37{\%}) were cleared of infection. MRSA isolates from these patients were all sensitive to acidified nitrite in vitro compared to methicillin-sensitive S. aureus and a reference strain of MRSA. ConclusionsNitric oxide and acidified nitrite offer a novel therapy for control of MRSA in wounds. Wounds that were not cleared of infection may have been re-contaminated or the bioavailability of acidified nitrite impaired by local factors in the tissue.",
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AU - Ferguson, Gail P

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