An Ultrastructural Study Of Cellular Response To Variation In Porosity In Phase-Pure Hydroxyapatite

Basil Annaz, K. A. Hing, M. Kayser, T. Buckland, L. Di Silvio

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30 Citations (Scopus)

Abstract

Hydroxyapatite has been shown to be biocompatible and bioactive. Incorporation of porosity has been shown to enhance osteointegration; however, difficulty in controlling the extent and type of porosity has limited investigation into determining the role of both macro- and microporosity. The current investigation reports on the synthesis of four types of phase-pure hydroxyapatite with varying levels of porosity (HA1-HA4), and with defined levels of macro- and microporosities. Transmission electron microscopy was used to evaluate qualitatively the effect of these two parameters on cell-material interactions following a 30-day incubation period. Biological mineralization was observed within vesicles and the needle-like minerals were confirmed as hydroxyapatite using X-ray microanalysis. This demonstrated the suitability of primary human osteoblast-like cells as a tool to assess the extent of mineralization. Furthermore, internalization of hydroxyapatite particles was observed. Our findings show that the variation in macro- and microporosity does not affect the extent of cell-material interaction, with collagen synthesis evident in all samples.

Original languageEnglish
Pages (from-to)97-109
Number of pages12
JournalJournal of Microscopy
Volume216
DOIs
Publication statusPublished - 2004

Keywords

  • hydroxyapatite
  • macroporosity
  • microporosity
  • osteoblasts
  • transmission electron microscopy
  • POROUS HYDROXYAPATITE
  • BETA-GLYCEROPHOSPHATE
  • OSTEOBLAST PHENOTYPE
  • BONE INGROWTH
  • PARTICLES
  • INVITRO
  • GROWTH
  • DIFFERENTIATION
  • MORPHOLOGY
  • CERAMICS

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