An Ultrastructural Study Of Cellular Response To Variation In Porosity In Phase-Pure Hydroxyapatite

Basil Annaz, K. A. Hing, M. Kayser, T. Buckland, L. Di Silvio

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Hydroxyapatite has been shown to be biocompatible and bioactive. Incorporation of porosity has been shown to enhance osteointegration; however, difficulty in controlling the extent and type of porosity has limited investigation into determining the role of both macro- and microporosity. The current investigation reports on the synthesis of four types of phase-pure hydroxyapatite with varying levels of porosity (HA1-HA4), and with defined levels of macro- and microporosities. Transmission electron microscopy was used to evaluate qualitatively the effect of these two parameters on cell-material interactions following a 30-day incubation period. Biological mineralization was observed within vesicles and the needle-like minerals were confirmed as hydroxyapatite using X-ray microanalysis. This demonstrated the suitability of primary human osteoblast-like cells as a tool to assess the extent of mineralization. Furthermore, internalization of hydroxyapatite particles was observed. Our findings show that the variation in macro- and microporosity does not affect the extent of cell-material interaction, with collagen synthesis evident in all samples.

Original languageEnglish
Pages (from-to)97-109
Number of pages12
JournalJournal of Microscopy
Volume216
DOIs
Publication statusPublished - 2004

Keywords

  • hydroxyapatite
  • macroporosity
  • microporosity
  • osteoblasts
  • transmission electron microscopy
  • POROUS HYDROXYAPATITE
  • BETA-GLYCEROPHOSPHATE
  • OSTEOBLAST PHENOTYPE
  • BONE INGROWTH
  • PARTICLES
  • INVITRO
  • GROWTH
  • DIFFERENTIATION
  • MORPHOLOGY
  • CERAMICS

Cite this

An Ultrastructural Study Of Cellular Response To Variation In Porosity In Phase-Pure Hydroxyapatite. / Annaz, Basil; Hing, K. A.; Kayser, M.; Buckland, T.; Di Silvio, L.

In: Journal of Microscopy, Vol. 216, 2004, p. 97-109.

Research output: Contribution to journalArticle

Annaz, Basil ; Hing, K. A. ; Kayser, M. ; Buckland, T. ; Di Silvio, L. / An Ultrastructural Study Of Cellular Response To Variation In Porosity In Phase-Pure Hydroxyapatite. In: Journal of Microscopy. 2004 ; Vol. 216. pp. 97-109.
@article{547f81b6e9ad4bdfb939cba480d3f413,
title = "An Ultrastructural Study Of Cellular Response To Variation In Porosity In Phase-Pure Hydroxyapatite",
abstract = "Hydroxyapatite has been shown to be biocompatible and bioactive. Incorporation of porosity has been shown to enhance osteointegration; however, difficulty in controlling the extent and type of porosity has limited investigation into determining the role of both macro- and microporosity. The current investigation reports on the synthesis of four types of phase-pure hydroxyapatite with varying levels of porosity (HA1-HA4), and with defined levels of macro- and microporosities. Transmission electron microscopy was used to evaluate qualitatively the effect of these two parameters on cell-material interactions following a 30-day incubation period. Biological mineralization was observed within vesicles and the needle-like minerals were confirmed as hydroxyapatite using X-ray microanalysis. This demonstrated the suitability of primary human osteoblast-like cells as a tool to assess the extent of mineralization. Furthermore, internalization of hydroxyapatite particles was observed. Our findings show that the variation in macro- and microporosity does not affect the extent of cell-material interaction, with collagen synthesis evident in all samples.",
keywords = "hydroxyapatite, macroporosity, microporosity, osteoblasts, transmission electron microscopy, POROUS HYDROXYAPATITE, BETA-GLYCEROPHOSPHATE, OSTEOBLAST PHENOTYPE, BONE INGROWTH, PARTICLES, INVITRO, GROWTH, DIFFERENTIATION, MORPHOLOGY, CERAMICS",
author = "Basil Annaz and Hing, {K. A.} and M. Kayser and T. Buckland and {Di Silvio}, L.",
year = "2004",
doi = "10.1111/j.0022-2720.2004.01403.x",
language = "English",
volume = "216",
pages = "97--109",
journal = "Journal of Microscopy",
issn = "0022-2720",
publisher = "Wiley-Blackwell",

}

TY - JOUR

T1 - An Ultrastructural Study Of Cellular Response To Variation In Porosity In Phase-Pure Hydroxyapatite

AU - Annaz, Basil

AU - Hing, K. A.

AU - Kayser, M.

AU - Buckland, T.

AU - Di Silvio, L.

PY - 2004

Y1 - 2004

N2 - Hydroxyapatite has been shown to be biocompatible and bioactive. Incorporation of porosity has been shown to enhance osteointegration; however, difficulty in controlling the extent and type of porosity has limited investigation into determining the role of both macro- and microporosity. The current investigation reports on the synthesis of four types of phase-pure hydroxyapatite with varying levels of porosity (HA1-HA4), and with defined levels of macro- and microporosities. Transmission electron microscopy was used to evaluate qualitatively the effect of these two parameters on cell-material interactions following a 30-day incubation period. Biological mineralization was observed within vesicles and the needle-like minerals were confirmed as hydroxyapatite using X-ray microanalysis. This demonstrated the suitability of primary human osteoblast-like cells as a tool to assess the extent of mineralization. Furthermore, internalization of hydroxyapatite particles was observed. Our findings show that the variation in macro- and microporosity does not affect the extent of cell-material interaction, with collagen synthesis evident in all samples.

AB - Hydroxyapatite has been shown to be biocompatible and bioactive. Incorporation of porosity has been shown to enhance osteointegration; however, difficulty in controlling the extent and type of porosity has limited investigation into determining the role of both macro- and microporosity. The current investigation reports on the synthesis of four types of phase-pure hydroxyapatite with varying levels of porosity (HA1-HA4), and with defined levels of macro- and microporosities. Transmission electron microscopy was used to evaluate qualitatively the effect of these two parameters on cell-material interactions following a 30-day incubation period. Biological mineralization was observed within vesicles and the needle-like minerals were confirmed as hydroxyapatite using X-ray microanalysis. This demonstrated the suitability of primary human osteoblast-like cells as a tool to assess the extent of mineralization. Furthermore, internalization of hydroxyapatite particles was observed. Our findings show that the variation in macro- and microporosity does not affect the extent of cell-material interaction, with collagen synthesis evident in all samples.

KW - hydroxyapatite

KW - macroporosity

KW - microporosity

KW - osteoblasts

KW - transmission electron microscopy

KW - POROUS HYDROXYAPATITE

KW - BETA-GLYCEROPHOSPHATE

KW - OSTEOBLAST PHENOTYPE

KW - BONE INGROWTH

KW - PARTICLES

KW - INVITRO

KW - GROWTH

KW - DIFFERENTIATION

KW - MORPHOLOGY

KW - CERAMICS

U2 - 10.1111/j.0022-2720.2004.01403.x

DO - 10.1111/j.0022-2720.2004.01403.x

M3 - Article

VL - 216

SP - 97

EP - 109

JO - Journal of Microscopy

JF - Journal of Microscopy

SN - 0022-2720

ER -