Analysis of adipose tissue immune gene expression after vaccination of rainbow trout with adjuvanted bacterins reveals an association with side effects

Kimberly A Veenstra; Tiehui Wang; Ayham Alnabulsi; Alex Douglas; K Spencer Russell; Lincoln Tubbs; Juliette Ben Arous; Christopher J Secombes

doi:10.1016/j.molimm.2017.05.026

Analysis of adipose tissue immune gene expression after vaccination of rainbow trout with adjuvanted bacterins reveals an association with side effects

Kimberly A Veenstra, Tiehui Wang, Ayham Alnabulsi, Alex Douglas, K Spencer Russell, Lincoln Tubbs, Juliette Ben Arous, Christopher J Secombes

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

43 Downloads (Pure)

Abstract

Most existing fish vaccines are presented in the form of oil-based emulsions delivered by intraperitoneal injection. Whilst very effective they are frequently associated with inflammatory responses that can result in clinically significant side-effects often involving the adipose tissue that is in direct contact with the vaccine. To explore the potential of immune gene expression changes in the adipose tissue of fish to be markers of vaccination efficacy or development of side-effects we have studied the response to a bacterial (Aeromonas salmonicida) vaccine administered with two different adjuvants. The first adjuvant was Montanide™ ISA 763A VG, thought to induce a mostly humoral response, and the second was Montanide™ ISA 761 VG that gives a more balanced humoral and cell mediated response. Following vaccination tissue samples were collected at days 3, 14 and 28 for RTqPCR analysis. Fifty immune genes were studied with a focus on a) pro-inflammatory associated molecules and b) adaptive immune response related molecules linked with possible Th1, Th2, Th17 and T-regulatory pathways, with the expression data analysed for associations with Speilberg post-vaccination side effect scores. The results showed that the adipose tissue is a particularly sensitive and discriminatory tissue for studying adjuvant effects. A clear upregulation of many immune genes occurred in response to both vaccine groups, which persisted over time and overlapped with the appearance of visible adhesions. Our analysis revealed a relationship between adipose tissue immune function and the development of vaccine-induced adhesions giving the potential to use immune gene expression profiling in this tissue to predict the side-effects seen.

Original language	English
Pages (from-to)	89-98
Number of pages	10
Journal	Molecular Immunology
Volume	88
Early online date	13 Jun 2017
DOIs	https://doi.org/10.1016/j.molimm.2017.05.026
Publication status	Published - Aug 2017

Bibliographical note

Acknowledgements
KAV was supported by ELANCO andSEPPIC to undertake a PhD program at the University of Aberdeen. TH received funding from the MASTS pooling initiative (The Marine Alliance for Science and Technology for Scotland). MASTS is funded by the Scottish Funding Council (grant reference HR09011).

Keywords

Journal Article
Water-in-oil adjuvant
Rainbow trout
Gene expression
Adipose tissue
Side-effects
Adhesions

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.molimm.2017.05.026Licence: Unspecified

Accepted Manuscript
© 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Accepted author manuscript, 89.7 KBLicence: CC BY-NC-ND
Fig 1Accepted author manuscript, 129 KBLicence: CC BY-NC-ND
Figure 2Accepted author manuscript, 880 KBLicence: CC BY-NC-ND
Figure 3Accepted author manuscript, 4.68 MBLicence: CC BY-NC-ND
Figure 4Accepted author manuscript, 4.3 MBLicence: CC BY-NC-ND
Figure 5Accepted author manuscript, 4.47 MBLicence: CC BY-NC-ND
Figure 6Accepted author manuscript, 733 KBLicence: CC BY-NC-ND

Cite this

@article{6f6a2f9082984b8184ea8772df951105,

title = "Analysis of adipose tissue immune gene expression after vaccination of rainbow trout with adjuvanted bacterins reveals an association with side effects",

abstract = "Most existing fish vaccines are presented in the form of oil-based emulsions delivered by intraperitoneal injection. Whilst very effective they are frequently associated with inflammatory responses that can result in clinically significant side-effects often involving the adipose tissue that is in direct contact with the vaccine. To explore the potential of immune gene expression changes in the adipose tissue of fish to be markers of vaccination efficacy or development of side-effects we have studied the response to a bacterial (Aeromonas salmonicida) vaccine administered with two different adjuvants. The first adjuvant was Montanide{\texttrademark} ISA 763A VG, thought to induce a mostly humoral response, and the second was Montanide{\texttrademark} ISA 761 VG that gives a more balanced humoral and cell mediated response. Following vaccination tissue samples were collected at days 3, 14 and 28 for RTqPCR analysis. Fifty immune genes were studied with a focus on a) pro-inflammatory associated molecules and b) adaptive immune response related molecules linked with possible Th1, Th2, Th17 and T-regulatory pathways, with the expression data analysed for associations with Speilberg post-vaccination side effect scores. The results showed that the adipose tissue is a particularly sensitive and discriminatory tissue for studying adjuvant effects. A clear upregulation of many immune genes occurred in response to both vaccine groups, which persisted over time and overlapped with the appearance of visible adhesions. Our analysis revealed a relationship between adipose tissue immune function and the development of vaccine-induced adhesions giving the potential to use immune gene expression profiling in this tissue to predict the side-effects seen.",

keywords = "Journal Article, Water-in-oil adjuvant, Rainbow trout, Gene expression, Adipose tissue, Side-effects, Adhesions",

author = "Veenstra, {Kimberly A} and Tiehui Wang and Ayham Alnabulsi and Alex Douglas and Russell, {K Spencer} and Lincoln Tubbs and Arous, {Juliette Ben} and Secombes, {Christopher J}",

note = "Acknowledgements KAV was supported by ELANCO andSEPPIC to undertake a PhD program at the University of Aberdeen. TH received funding from the MASTS pooling initiative (The Marine Alliance for Science and Technology for Scotland). MASTS is funded by the Scottish Funding Council (grant reference HR09011).",

year = "2017",

month = aug,

doi = "10.1016/j.molimm.2017.05.026",

language = "English",

volume = "88",

pages = "89--98",

journal = "Molecular Immunology",

issn = "0161-5890",

publisher = "PERGAMON-ELSEVIER SCIENCE LTD",

}

TY - JOUR

T1 - Analysis of adipose tissue immune gene expression after vaccination of rainbow trout with adjuvanted bacterins reveals an association with side effects

AU - Veenstra, Kimberly A

AU - Wang, Tiehui

AU - Alnabulsi, Ayham

AU - Douglas, Alex

AU - Russell, K Spencer

AU - Tubbs, Lincoln

AU - Arous, Juliette Ben

AU - Secombes, Christopher J

N1 - Acknowledgements KAV was supported by ELANCO andSEPPIC to undertake a PhD program at the University of Aberdeen. TH received funding from the MASTS pooling initiative (The Marine Alliance for Science and Technology for Scotland). MASTS is funded by the Scottish Funding Council (grant reference HR09011).

PY - 2017/8

Y1 - 2017/8

N2 - Most existing fish vaccines are presented in the form of oil-based emulsions delivered by intraperitoneal injection. Whilst very effective they are frequently associated with inflammatory responses that can result in clinically significant side-effects often involving the adipose tissue that is in direct contact with the vaccine. To explore the potential of immune gene expression changes in the adipose tissue of fish to be markers of vaccination efficacy or development of side-effects we have studied the response to a bacterial (Aeromonas salmonicida) vaccine administered with two different adjuvants. The first adjuvant was Montanide™ ISA 763A VG, thought to induce a mostly humoral response, and the second was Montanide™ ISA 761 VG that gives a more balanced humoral and cell mediated response. Following vaccination tissue samples were collected at days 3, 14 and 28 for RTqPCR analysis. Fifty immune genes were studied with a focus on a) pro-inflammatory associated molecules and b) adaptive immune response related molecules linked with possible Th1, Th2, Th17 and T-regulatory pathways, with the expression data analysed for associations with Speilberg post-vaccination side effect scores. The results showed that the adipose tissue is a particularly sensitive and discriminatory tissue for studying adjuvant effects. A clear upregulation of many immune genes occurred in response to both vaccine groups, which persisted over time and overlapped with the appearance of visible adhesions. Our analysis revealed a relationship between adipose tissue immune function and the development of vaccine-induced adhesions giving the potential to use immune gene expression profiling in this tissue to predict the side-effects seen.

AB - Most existing fish vaccines are presented in the form of oil-based emulsions delivered by intraperitoneal injection. Whilst very effective they are frequently associated with inflammatory responses that can result in clinically significant side-effects often involving the adipose tissue that is in direct contact with the vaccine. To explore the potential of immune gene expression changes in the adipose tissue of fish to be markers of vaccination efficacy or development of side-effects we have studied the response to a bacterial (Aeromonas salmonicida) vaccine administered with two different adjuvants. The first adjuvant was Montanide™ ISA 763A VG, thought to induce a mostly humoral response, and the second was Montanide™ ISA 761 VG that gives a more balanced humoral and cell mediated response. Following vaccination tissue samples were collected at days 3, 14 and 28 for RTqPCR analysis. Fifty immune genes were studied with a focus on a) pro-inflammatory associated molecules and b) adaptive immune response related molecules linked with possible Th1, Th2, Th17 and T-regulatory pathways, with the expression data analysed for associations with Speilberg post-vaccination side effect scores. The results showed that the adipose tissue is a particularly sensitive and discriminatory tissue for studying adjuvant effects. A clear upregulation of many immune genes occurred in response to both vaccine groups, which persisted over time and overlapped with the appearance of visible adhesions. Our analysis revealed a relationship between adipose tissue immune function and the development of vaccine-induced adhesions giving the potential to use immune gene expression profiling in this tissue to predict the side-effects seen.

KW - Journal Article

KW - Water-in-oil adjuvant

KW - Rainbow trout

KW - Gene expression

KW - Adipose tissue

KW - Side-effects

KW - Adhesions

U2 - 10.1016/j.molimm.2017.05.026

DO - 10.1016/j.molimm.2017.05.026

M3 - Article

C2 - 28622574

SN - 0161-5890

VL - 88

SP - 89

EP - 98

JO - Molecular Immunology

JF - Molecular Immunology

ER -

Analysis of adipose tissue immune gene expression after vaccination of rainbow trout with adjuvanted bacterins reveals an association with side effects

Abstract

Bibliographical note

Keywords

UN SDGs

Access to Document

Fingerprint

Cite this