Clodronate belongs to the family of bisphosphonates, which are synthetic analogues of pyrophosphate. Bisphosphonates are widely used in the treatment of metabolic bone diseases. Some bisphosphonates, including clodronate, can be metabolized in cells into non-hydrolysable nucleotide analogues. In this paper. we describe a new method for extraction and quantitation of the clodronate metabolite in cell lysates by using ion-pairing HPLC method that is compatible with negative ion electrospray ionization mass spectrometry (ESI-MS). The method was used for detection of the metabolite of clodronate in extracts from RAW 264 macrophage cells after treatment with clodronate. (C) 2000 Elsevier Science B.V. All rights reserved.
|Number of pages||9|
|Journal||Journal of Chromatography B: Biomedical Sciences and Applications|
|Publication status||Published - 2000|
- MOLD DICTYOSTELIUM-DISCOIDEUM