Analysis of an adenine nucleotide-containing metabolite of clodronate using ion pair high-performance liquid chromatography-electrospray ionisation mass spectrometry

H Monkkonen, P Moilanen, J Monkkonen, J C Frith, M J Rogers, S Auriola

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Clodronate belongs to the family of bisphosphonates, which are synthetic analogues of pyrophosphate. Bisphosphonates are widely used in the treatment of metabolic bone diseases. Some bisphosphonates, including clodronate, can be metabolized in cells into non-hydrolysable nucleotide analogues. In this paper. we describe a new method for extraction and quantitation of the clodronate metabolite in cell lysates by using ion-pairing HPLC method that is compatible with negative ion electrospray ionization mass spectrometry (ESI-MS). The method was used for detection of the metabolite of clodronate in extracts from RAW 264 macrophage cells after treatment with clodronate. (C) 2000 Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)395-403
Number of pages9
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume738
Publication statusPublished - 2000

Keywords

  • clodronate
  • MOLD DICTYOSTELIUM-DISCOIDEUM
  • IN-VITRO
  • BISPHOSPHONATES
  • AMEBAS

Cite this

Analysis of an adenine nucleotide-containing metabolite of clodronate using ion pair high-performance liquid chromatography-electrospray ionisation mass spectrometry. / Monkkonen, H ; Moilanen, P ; Monkkonen, J ; Frith, J C ; Rogers, M J ; Auriola, S .

In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 738, 2000, p. 395-403.

Research output: Contribution to journalArticle

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T1 - Analysis of an adenine nucleotide-containing metabolite of clodronate using ion pair high-performance liquid chromatography-electrospray ionisation mass spectrometry

AU - Monkkonen, H

AU - Moilanen, P

AU - Monkkonen, J

AU - Frith, J C

AU - Rogers, M J

AU - Auriola, S

PY - 2000

Y1 - 2000

N2 - Clodronate belongs to the family of bisphosphonates, which are synthetic analogues of pyrophosphate. Bisphosphonates are widely used in the treatment of metabolic bone diseases. Some bisphosphonates, including clodronate, can be metabolized in cells into non-hydrolysable nucleotide analogues. In this paper. we describe a new method for extraction and quantitation of the clodronate metabolite in cell lysates by using ion-pairing HPLC method that is compatible with negative ion electrospray ionization mass spectrometry (ESI-MS). The method was used for detection of the metabolite of clodronate in extracts from RAW 264 macrophage cells after treatment with clodronate. (C) 2000 Elsevier Science B.V. All rights reserved.

AB - Clodronate belongs to the family of bisphosphonates, which are synthetic analogues of pyrophosphate. Bisphosphonates are widely used in the treatment of metabolic bone diseases. Some bisphosphonates, including clodronate, can be metabolized in cells into non-hydrolysable nucleotide analogues. In this paper. we describe a new method for extraction and quantitation of the clodronate metabolite in cell lysates by using ion-pairing HPLC method that is compatible with negative ion electrospray ionization mass spectrometry (ESI-MS). The method was used for detection of the metabolite of clodronate in extracts from RAW 264 macrophage cells after treatment with clodronate. (C) 2000 Elsevier Science B.V. All rights reserved.

KW - clodronate

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M3 - Article

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JO - Journal of Chromatography B

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