Analysis of Gene Expression in Xenopus Embryos

Danielle Lavery, Stefan Hoppler

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Citations (Scopus)

Abstract

Determining the expression pattern of a gene of interest is critical to understanding when, where, and how it may function during development. This chapter describes methods for determining the localization and expression levels for both mRNA and protein. Some of these methods can be described as quantitative or semi-quantitative while others are considered more qualitative in nature. To determine the spatial localization of mRNA expression, RNA in situ hybridization can be used on both whole-mount or sectioned embryos. Northern blot and qPCR are more quantitative methods for analyzing mRNA expression levels. For determining protein localization, antibody staining either by immunocytochemistry or immunofluorescence can be performed on both whole-mount or sectioned embryos; whereas Western blot is the method generally used for quantifying protein expression levels.
Original languageEnglish
Title of host publicationWnt Signaling
Subtitle of host publicationVolume 2, Pathway Models
EditorsElizabeth Vincan
Place of PublicationTotowa, NJ, USA
PublisherHumana Press
Pages335-361
Number of pages27
Volume469 (2)
ISBN (Print)1603274685 , 978-1603274685
DOIs
Publication statusPublished - 23 Oct 2008

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Number2
Volume469
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • xenopus
  • RNA
  • histology
  • antibody
  • northern blot
  • western blot
  • in situ
  • immunocy-tochemistry

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  • Cite this

    Lavery, D., & Hoppler, S. (2008). Analysis of Gene Expression in Xenopus Embryos. In E. Vincan (Ed.), Wnt Signaling: Volume 2, Pathway Models (Vol. 469 (2), pp. 335-361). (Methods in Molecular Biology; Vol. 469, No. 2). Humana Press. https://doi.org/10.1007/978-1-60327-469-2_22