Analysis of GPCR localization and trafficking

James N Hislop, Mark von Zastrow

Research output: Chapter in Book/Report/Conference proceedingChapter

12 Citations (Scopus)

Abstract

Localization and trafficking of G protein-coupled receptors (GPCRs) is increasingly recognized to play a fundamental role in receptor-mediated signaling and its regulation. Individual receptors, including closely homologous subtypes with otherwise similar functional properties, can differ considerably in their membrane trafficking properties. In this chapter, we describe several approaches for experimentally assessing the subcellular localization and trafficking of selected GPCRs. Firstly, we describe a flexible method for receptor localization using fluorescence microscopy. We then describe two complementary approaches, using fluorescence flow cytometry and surface biotinylation, for examining receptor internalization and trafficking in the endocytic pathway.
Original languageEnglish
Title of host publicationReceptor Signal Transduction Protocols
Subtitle of host publicationThird Edition
EditorsGary B Willars, R A John Challiss
PublisherHumana Press
Pages425-440
Number of pages16
Volume746
ISBN (Electronic)978-1-61779-126-0
ISBN (Print)978-1-61779-125-3
DOIs
Publication statusPublished - 2011

Publication series

NameMethods in Molecular Biology: Methods and Protocols
Volume746
ISSN (Print)1064-3745

Fingerprint

G-Protein-Coupled Receptors
Biotinylation
Fluorescence Microscopy
Flow Cytometry
Fluorescence
Membranes

Keywords

  • biotinylation
  • flow cytometry
  • HEK293 cells
  • humans
  • intracellular space
  • protein transport
  • receptors, G-protein-coupled
  • staining and labeling
  • tissue fixation

Cite this

Hislop, J. N., & von Zastrow, M. (2011). Analysis of GPCR localization and trafficking. In G. B. Willars, & R. A. J. Challiss (Eds.), Receptor Signal Transduction Protocols: Third Edition (Vol. 746, pp. 425-440). (Methods in Molecular Biology: Methods and Protocols; Vol. 746). Humana Press. https://doi.org/10.1007/978-1-61779-126-0_25

Analysis of GPCR localization and trafficking. / Hislop, James N; von Zastrow, Mark.

Receptor Signal Transduction Protocols: Third Edition. ed. / Gary B Willars; R A John Challiss. Vol. 746 Humana Press, 2011. p. 425-440 (Methods in Molecular Biology: Methods and Protocols; Vol. 746).

Research output: Chapter in Book/Report/Conference proceedingChapter

Hislop, JN & von Zastrow, M 2011, Analysis of GPCR localization and trafficking. in GB Willars & RAJ Challiss (eds), Receptor Signal Transduction Protocols: Third Edition. vol. 746, Methods in Molecular Biology: Methods and Protocols, vol. 746, Humana Press, pp. 425-440. https://doi.org/10.1007/978-1-61779-126-0_25
Hislop JN, von Zastrow M. Analysis of GPCR localization and trafficking. In Willars GB, Challiss RAJ, editors, Receptor Signal Transduction Protocols: Third Edition. Vol. 746. Humana Press. 2011. p. 425-440. (Methods in Molecular Biology: Methods and Protocols). https://doi.org/10.1007/978-1-61779-126-0_25
Hislop, James N ; von Zastrow, Mark. / Analysis of GPCR localization and trafficking. Receptor Signal Transduction Protocols: Third Edition. editor / Gary B Willars ; R A John Challiss. Vol. 746 Humana Press, 2011. pp. 425-440 (Methods in Molecular Biology: Methods and Protocols).
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