Abstract
The study of the murine macrophage mannose receptor (MR) has been hampered by the lack of specific reagents. We have generated and characterized novel anti-MR monoclonal antibodies and used them to analyze MR expression in primary mouse macrophages (MO). In BioGel- and thioglycollate-elicited MO, interleukin (IL)-4 up-regulated total cell-associated MR (cMR), correlating with enhanced surface expression. We investigated the influence of IL-10, a well-characterized deactivator of MO function, on MR levels and observed that it had a similar effect to IL-4. In both cases, enhanced cMR levels translated into increased production of the soluble form of the receptor (sMR). We have demonstrated the presence of sMR in cultures of stable non-MO transductants expressing full-length MR, indicating that the proteolytic activity responsible for cMR cleavage is not MO-restricted. These data support a role for the MR in T helper cell type 2 cytokine-driven, immune responses and suggest a non-MO contribution to sMR production in vivo.
Original language | English |
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Pages (from-to) | 604-613 |
Number of pages | 9 |
Journal | Journal of Leukocyte Biology |
Volume | 73 |
DOIs | |
Publication status | Published - 2003 |
Keywords
- macrophage
- shedding
- CYSTEINE-RICH DOMAIN
- CARBOHYDRATE-RECOGNITION DOMAINS
- MACROPHAGES IN-VITRO
- BETA 1,4GLCNAC BETA
- B-CELL FOLLICLES
- DENDRITIC CELLS
- MURINE MACROPHAGES
- INTERFERON-GAMMA
- HOST-DEFENSE
- ENDOTHELIAL-CELLS