Analysis of mannose receptor regulation by IL-4, IL-10, and proteolytic processing using novel monoclonal antibodies

L. Martinez-Pomares, D. M. Reid, G. D. Brown, P. R. Taylor, R. J. Stillion, S. A. Linehan, S. Zamze, S. Gordon, Simon Yuk Chun Wong

Research output: Contribution to journalArticle

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Abstract

The study of the murine macrophage mannose receptor (MR) has been hampered by the lack of specific reagents. We have generated and characterized novel anti-MR monoclonal antibodies and used them to analyze MR expression in primary mouse macrophages (MO). In BioGel- and thioglycollate-elicited MO, interleukin (IL)-4 up-regulated total cell-associated MR (cMR), correlating with enhanced surface expression. We investigated the influence of IL-10, a well-characterized deactivator of MO function, on MR levels and observed that it had a similar effect to IL-4. In both cases, enhanced cMR levels translated into increased production of the soluble form of the receptor (sMR). We have demonstrated the presence of sMR in cultures of stable non-MO transductants expressing full-length MR, indicating that the proteolytic activity responsible for cMR cleavage is not MO-restricted. These data support a role for the MR in T helper cell type 2 cytokine-driven, immune responses and suggest a non-MO contribution to sMR production in vivo.

Original languageEnglish
Pages (from-to)604-613
Number of pages9
JournalJournal of Leukocyte Biology
Volume73
DOIs
Publication statusPublished - 2003

Keywords

  • macrophage
  • shedding
  • CYSTEINE-RICH DOMAIN
  • CARBOHYDRATE-RECOGNITION DOMAINS
  • MACROPHAGES IN-VITRO
  • BETA 1,4GLCNAC BETA
  • B-CELL FOLLICLES
  • DENDRITIC CELLS
  • MURINE MACROPHAGES
  • INTERFERON-GAMMA
  • HOST-DEFENSE
  • ENDOTHELIAL-CELLS

Cite this

Martinez-Pomares, L., Reid, D. M., Brown, G. D., Taylor, P. R., Stillion, R. J., Linehan, S. A., ... Wong, S. Y. C. (2003). Analysis of mannose receptor regulation by IL-4, IL-10, and proteolytic processing using novel monoclonal antibodies. Journal of Leukocyte Biology, 73, 604-613. https://doi.org/10.1189/jlb.0902450

Analysis of mannose receptor regulation by IL-4, IL-10, and proteolytic processing using novel monoclonal antibodies. / Martinez-Pomares, L.; Reid, D. M.; Brown, G. D.; Taylor, P. R.; Stillion, R. J.; Linehan, S. A.; Zamze, S.; Gordon, S.; Wong, Simon Yuk Chun.

In: Journal of Leukocyte Biology, Vol. 73, 2003, p. 604-613.

Research output: Contribution to journalArticle

Martinez-Pomares, L, Reid, DM, Brown, GD, Taylor, PR, Stillion, RJ, Linehan, SA, Zamze, S, Gordon, S & Wong, SYC 2003, 'Analysis of mannose receptor regulation by IL-4, IL-10, and proteolytic processing using novel monoclonal antibodies', Journal of Leukocyte Biology, vol. 73, pp. 604-613. https://doi.org/10.1189/jlb.0902450
Martinez-Pomares, L. ; Reid, D. M. ; Brown, G. D. ; Taylor, P. R. ; Stillion, R. J. ; Linehan, S. A. ; Zamze, S. ; Gordon, S. ; Wong, Simon Yuk Chun. / Analysis of mannose receptor regulation by IL-4, IL-10, and proteolytic processing using novel monoclonal antibodies. In: Journal of Leukocyte Biology. 2003 ; Vol. 73. pp. 604-613.
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abstract = "The study of the murine macrophage mannose receptor (MR) has been hampered by the lack of specific reagents. We have generated and characterized novel anti-MR monoclonal antibodies and used them to analyze MR expression in primary mouse macrophages (MO). In BioGel- and thioglycollate-elicited MO, interleukin (IL)-4 up-regulated total cell-associated MR (cMR), correlating with enhanced surface expression. We investigated the influence of IL-10, a well-characterized deactivator of MO function, on MR levels and observed that it had a similar effect to IL-4. In both cases, enhanced cMR levels translated into increased production of the soluble form of the receptor (sMR). We have demonstrated the presence of sMR in cultures of stable non-MO transductants expressing full-length MR, indicating that the proteolytic activity responsible for cMR cleavage is not MO-restricted. These data support a role for the MR in T helper cell type 2 cytokine-driven, immune responses and suggest a non-MO contribution to sMR production in vivo.",
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AU - Wong, Simon Yuk Chun

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AB - The study of the murine macrophage mannose receptor (MR) has been hampered by the lack of specific reagents. We have generated and characterized novel anti-MR monoclonal antibodies and used them to analyze MR expression in primary mouse macrophages (MO). In BioGel- and thioglycollate-elicited MO, interleukin (IL)-4 up-regulated total cell-associated MR (cMR), correlating with enhanced surface expression. We investigated the influence of IL-10, a well-characterized deactivator of MO function, on MR levels and observed that it had a similar effect to IL-4. In both cases, enhanced cMR levels translated into increased production of the soluble form of the receptor (sMR). We have demonstrated the presence of sMR in cultures of stable non-MO transductants expressing full-length MR, indicating that the proteolytic activity responsible for cMR cleavage is not MO-restricted. These data support a role for the MR in T helper cell type 2 cytokine-driven, immune responses and suggest a non-MO contribution to sMR production in vivo.

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