Analysis of the diversity of a sheep antibody repertoire as revealed from a bacteriophage display library

K A Charlton, S Moyle, A J R Porter, W J Harris

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

We have applied bacteriophage display technology to construct and analyze the diversity of an IgG library of >1 x 10(8) clones from an adult sheep immunized against the hapten atrazine. We have identified eight new V-H gene families (V(H)2-V(H)9) and five new V kappa gene families (V kappa V-V kappa IX). The heavy and kappa light chain variable region gene loci were found to be far more diverse than previously thought.

Original languageEnglish
Pages (from-to)6221-6229
Number of pages9
JournalThe Journal of Immunology
Volume164
Publication statusPublished - 2000

Keywords

  • CHAIN VARIABLE REGION
  • H GENE FAMILY
  • CHICKEN IMMUNOGLOBULIN GENES
  • PHAGE-DISPLAY
  • HEAVY-CHAINS
  • VH-GENE
  • GENERATION
  • CATTLE
  • DIVERSIFICATION
  • ORGANIZATION

Cite this