Analysis of the Root System Architecture of Arabidopsis Provides a Quantitative Readout of Crosstalk between Nutritional Signals

Fabian Kellermeier, Patrick Armengaud, Triona J Seditas, John Danku, David E Salt, Anna Amtmann

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

As plant roots forage the soil for food and water, they translate a multifactorial input of environmental stimuli into a multifactorial developmental output that manifests itself as root system architecture (RSA). Our current understanding of the underlying regulatory network is limited because root responses have traditionally been studied separately for individual nutrient deficiencies. In this study, we quantified 13 RSA parameters of Arabidopsis thaliana in 32 binary combinations of N, P, K, S, and light. Analysis of variance showed that each RSA parameter was determined by a typical pattern of environmental signals and their interactions. P caused the most important single-nutrient effects, while N-effects were strongly light dependent. Effects of K and S occurred mostly through nutrient interactions in paired or multiple combinations. Several RSA parameters were selected for further analysis through mutant phenotyping, which revealed combinations of transporters, receptors, and kinases acting as signaling modules in K-N interactions. Furthermore, nutrient response profiles of individual RSA features across NPK combinations could be assigned to transcriptionally coregulated clusters of nutrient-responsive genes in the roots and to ionome patterns in the shoots. The obtained data set provides a quantitative basis for understanding how plants integrate multiple nutritional stimuli into complex developmental programs.
Original languageEnglish
Pages (from-to)1480-1496
Number of pages17
JournalThe Plant Cell
Volume26
Issue number4
Early online date1 Apr 2014
DOIs
Publication statusPublished - Apr 2014

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Systems Analysis
Arabidopsis
root systems
Food
nutrients
nutrient-nutrient interactions
Light
nutrient deficiencies
Plant Roots
transporters
phosphotransferases (kinases)
Arabidopsis thaliana
analysis of variance
forage
Analysis of Variance
Phosphotransferases
Soil
phenotype
mutants
receptors

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Analysis of the Root System Architecture of Arabidopsis Provides a Quantitative Readout of Crosstalk between Nutritional Signals. / Kellermeier, Fabian; Armengaud, Patrick; Seditas, Triona J; Danku, John; Salt, David E; Amtmann, Anna.

In: The Plant Cell, Vol. 26, No. 4, 04.2014, p. 1480-1496.

Research output: Contribution to journalArticle

Kellermeier, Fabian ; Armengaud, Patrick ; Seditas, Triona J ; Danku, John ; Salt, David E ; Amtmann, Anna. / Analysis of the Root System Architecture of Arabidopsis Provides a Quantitative Readout of Crosstalk between Nutritional Signals. In: The Plant Cell. 2014 ; Vol. 26, No. 4. pp. 1480-1496.
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