Anti-cancer activities of allyl isothiocyanate and its conjugated silicon quantum dots

Peng Liu, Mehrnaz Behray, Qi Wang, Wei Wang, Zhigang Zhou, Yimin Chao, Yongping Bao (Corresponding Author)

Research output: Contribution to journalArticle

Abstract

Allyl isothiocyanate (AITC), a dietary phytochemical in some cruciferous vegetables, exhibits promising anticancer activities in many cancer models. However, previous data showed AITC to have a biphasic effect on cell viability, DNA damage and migration in human hepatoma HepG2 cells. Moreover, in a 3D co-culture of HUVEC with pericytes, it inhibited tube formation at high doses but promoted this at low doses, which confirmed its biphasic effect on angiogenesis. siRNA knockdown of Nrf2 and glutathione inhibition abolished the stimulation effect of AITC on cell migration and DNA damage. The biological activity of a novel AITC-conjugated silicon quantum dots (AITC-SiQDs) has been investigated for the first time. AITC-SiQDs showed similar anti-cancer properties to AITC at high doses while avoiding the low doses stimulation effect. In addition, AITC-SiQDs showed a lower and long-lasting activation of Nrf2 translocation into nucleus which correlated with their levels of cellular uptake, as detected by the intrinsic fluorescence of SiQDs. ROS production could be one of the mechanisms behind the anti-cancer effect of AITC-SiQDs. These data provide novel insights into the biphasic effect of AITC and highlight the application of nanotechnology to optimize the therapeutic potential of dietary isothiocyanates in cancer treatment.

Original languageEnglish
Article number1084
JournalScientific Reports
Volume8
DOIs
Publication statusPublished - 18 Jan 2018

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Quantum Dots
Silicon
Neoplasms
DNA Damage
Isothiocyanates
allyl isothiocyanate
Pericytes
Nanotechnology
Hep G2 Cells
Phytochemicals
Coculture Techniques
Vegetables
Small Interfering RNA
Cell Movement
Glutathione
Hepatocellular Carcinoma
Cell Survival
Fluorescence

Keywords

  • Antineoplastic Agents/chemistry
  • Apoptosis/drug effects
  • Cell Line, Tumor
  • Cell Movement/drug effects
  • Cell Proliferation/drug effects
  • Cell Survival/drug effects
  • Humans
  • Isothiocyanates/chemistry
  • NF-E2-Related Factor 2/metabolism
  • Neovascularization, Physiologic/drug effects
  • Quantum Dots/chemistry
  • Reactive Oxygen Species/metabolism
  • Silicon/chemistry

Cite this

Anti-cancer activities of allyl isothiocyanate and its conjugated silicon quantum dots. / Liu, Peng; Behray, Mehrnaz; Wang, Qi; Wang, Wei; Zhou, Zhigang; Chao, Yimin; Bao, Yongping (Corresponding Author).

In: Scientific Reports, Vol. 8, 1084, 18.01.2018.

Research output: Contribution to journalArticle

Liu, Peng ; Behray, Mehrnaz ; Wang, Qi ; Wang, Wei ; Zhou, Zhigang ; Chao, Yimin ; Bao, Yongping. / Anti-cancer activities of allyl isothiocyanate and its conjugated silicon quantum dots. In: Scientific Reports. 2018 ; Vol. 8.
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abstract = "Allyl isothiocyanate (AITC), a dietary phytochemical in some cruciferous vegetables, exhibits promising anticancer activities in many cancer models. However, previous data showed AITC to have a biphasic effect on cell viability, DNA damage and migration in human hepatoma HepG2 cells. Moreover, in a 3D co-culture of HUVEC with pericytes, it inhibited tube formation at high doses but promoted this at low doses, which confirmed its biphasic effect on angiogenesis. siRNA knockdown of Nrf2 and glutathione inhibition abolished the stimulation effect of AITC on cell migration and DNA damage. The biological activity of a novel AITC-conjugated silicon quantum dots (AITC-SiQDs) has been investigated for the first time. AITC-SiQDs showed similar anti-cancer properties to AITC at high doses while avoiding the low doses stimulation effect. In addition, AITC-SiQDs showed a lower and long-lasting activation of Nrf2 translocation into nucleus which correlated with their levels of cellular uptake, as detected by the intrinsic fluorescence of SiQDs. ROS production could be one of the mechanisms behind the anti-cancer effect of AITC-SiQDs. These data provide novel insights into the biphasic effect of AITC and highlight the application of nanotechnology to optimize the therapeutic potential of dietary isothiocyanates in cancer treatment.",
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AU - Liu, Peng

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AU - Wang, Wei

AU - Zhou, Zhigang

AU - Chao, Yimin

AU - Bao, Yongping

N1 - The authors are grateful to Dr. Richard Bowater in School of Biological Sciences, UEA, for the Comet assay assistance, Dr. Paul Thomas in Henry Welcome Laboratory for Cell Imaging, UEA, for the confocal microscopy assistance.

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Y1 - 2018/1/18

N2 - Allyl isothiocyanate (AITC), a dietary phytochemical in some cruciferous vegetables, exhibits promising anticancer activities in many cancer models. However, previous data showed AITC to have a biphasic effect on cell viability, DNA damage and migration in human hepatoma HepG2 cells. Moreover, in a 3D co-culture of HUVEC with pericytes, it inhibited tube formation at high doses but promoted this at low doses, which confirmed its biphasic effect on angiogenesis. siRNA knockdown of Nrf2 and glutathione inhibition abolished the stimulation effect of AITC on cell migration and DNA damage. The biological activity of a novel AITC-conjugated silicon quantum dots (AITC-SiQDs) has been investigated for the first time. AITC-SiQDs showed similar anti-cancer properties to AITC at high doses while avoiding the low doses stimulation effect. In addition, AITC-SiQDs showed a lower and long-lasting activation of Nrf2 translocation into nucleus which correlated with their levels of cellular uptake, as detected by the intrinsic fluorescence of SiQDs. ROS production could be one of the mechanisms behind the anti-cancer effect of AITC-SiQDs. These data provide novel insights into the biphasic effect of AITC and highlight the application of nanotechnology to optimize the therapeutic potential of dietary isothiocyanates in cancer treatment.

AB - Allyl isothiocyanate (AITC), a dietary phytochemical in some cruciferous vegetables, exhibits promising anticancer activities in many cancer models. However, previous data showed AITC to have a biphasic effect on cell viability, DNA damage and migration in human hepatoma HepG2 cells. Moreover, in a 3D co-culture of HUVEC with pericytes, it inhibited tube formation at high doses but promoted this at low doses, which confirmed its biphasic effect on angiogenesis. siRNA knockdown of Nrf2 and glutathione inhibition abolished the stimulation effect of AITC on cell migration and DNA damage. The biological activity of a novel AITC-conjugated silicon quantum dots (AITC-SiQDs) has been investigated for the first time. AITC-SiQDs showed similar anti-cancer properties to AITC at high doses while avoiding the low doses stimulation effect. In addition, AITC-SiQDs showed a lower and long-lasting activation of Nrf2 translocation into nucleus which correlated with their levels of cellular uptake, as detected by the intrinsic fluorescence of SiQDs. ROS production could be one of the mechanisms behind the anti-cancer effect of AITC-SiQDs. These data provide novel insights into the biphasic effect of AITC and highlight the application of nanotechnology to optimize the therapeutic potential of dietary isothiocyanates in cancer treatment.

KW - Antineoplastic Agents/chemistry

KW - Apoptosis/drug effects

KW - Cell Line, Tumor

KW - Cell Movement/drug effects

KW - Cell Proliferation/drug effects

KW - Cell Survival/drug effects

KW - Humans

KW - Isothiocyanates/chemistry

KW - NF-E2-Related Factor 2/metabolism

KW - Neovascularization, Physiologic/drug effects

KW - Quantum Dots/chemistry

KW - Reactive Oxygen Species/metabolism

KW - Silicon/chemistry

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