Abstract
Allyl isothiocyanate (AITC), a dietary phytochemical in some cruciferous vegetables, exhibits promising anticancer activities in many cancer models. However, previous data showed AITC to have a biphasic effect on cell viability, DNA damage and migration in human hepatoma HepG2 cells. Moreover, in a 3D co-culture of HUVEC with pericytes, it inhibited tube formation at high doses but promoted this at low doses, which confirmed its biphasic effect on angiogenesis. siRNA knockdown of Nrf2 and glutathione inhibition abolished the stimulation effect of AITC on cell migration and DNA damage. The biological activity of a novel AITC-conjugated silicon quantum dots (AITC-SiQDs) has been investigated for the first time. AITC-SiQDs showed similar anti-cancer properties to AITC at high doses while avoiding the low doses stimulation effect. In addition, AITC-SiQDs showed a lower and long-lasting activation of Nrf2 translocation into nucleus which correlated with their levels of cellular uptake, as detected by the intrinsic fluorescence of SiQDs. ROS production could be one of the mechanisms behind the anti-cancer effect of AITC-SiQDs. These data provide novel insights into the biphasic effect of AITC and highlight the application of nanotechnology to optimize the therapeutic potential of dietary isothiocyanates in cancer treatment.
| Original language | English |
|---|---|
| Article number | 1084 |
| Journal | Scientific Reports |
| Volume | 8 |
| DOIs | |
| Publication status | Published - 18 Jan 2018 |
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Keywords
- Antineoplastic Agents/chemistry
- Apoptosis/drug effects
- Cell Line, Tumor
- Cell Movement/drug effects
- Cell Proliferation/drug effects
- Cell Survival/drug effects
- Humans
- Isothiocyanates/chemistry
- NF-E2-Related Factor 2/metabolism
- Neovascularization, Physiologic/drug effects
- Quantum Dots/chemistry
- Reactive Oxygen Species/metabolism
- Silicon/chemistry
Cite this
Anti-cancer activities of allyl isothiocyanate and its conjugated silicon quantum dots. / Liu, Peng; Behray, Mehrnaz; Wang, Qi; Wang, Wei; Zhou, Zhigang; Chao, Yimin; Bao, Yongping (Corresponding Author).
In: Scientific Reports, Vol. 8, 1084, 18.01.2018.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Anti-cancer activities of allyl isothiocyanate and its conjugated silicon quantum dots
AU - Liu, Peng
AU - Behray, Mehrnaz
AU - Wang, Qi
AU - Wang, Wei
AU - Zhou, Zhigang
AU - Chao, Yimin
AU - Bao, Yongping
N1 - The authors are grateful to Dr. Richard Bowater in School of Biological Sciences, UEA, for the Comet assay assistance, Dr. Paul Thomas in Henry Welcome Laboratory for Cell Imaging, UEA, for the confocal microscopy assistance.
PY - 2018/1/18
Y1 - 2018/1/18
N2 - Allyl isothiocyanate (AITC), a dietary phytochemical in some cruciferous vegetables, exhibits promising anticancer activities in many cancer models. However, previous data showed AITC to have a biphasic effect on cell viability, DNA damage and migration in human hepatoma HepG2 cells. Moreover, in a 3D co-culture of HUVEC with pericytes, it inhibited tube formation at high doses but promoted this at low doses, which confirmed its biphasic effect on angiogenesis. siRNA knockdown of Nrf2 and glutathione inhibition abolished the stimulation effect of AITC on cell migration and DNA damage. The biological activity of a novel AITC-conjugated silicon quantum dots (AITC-SiQDs) has been investigated for the first time. AITC-SiQDs showed similar anti-cancer properties to AITC at high doses while avoiding the low doses stimulation effect. In addition, AITC-SiQDs showed a lower and long-lasting activation of Nrf2 translocation into nucleus which correlated with their levels of cellular uptake, as detected by the intrinsic fluorescence of SiQDs. ROS production could be one of the mechanisms behind the anti-cancer effect of AITC-SiQDs. These data provide novel insights into the biphasic effect of AITC and highlight the application of nanotechnology to optimize the therapeutic potential of dietary isothiocyanates in cancer treatment.
AB - Allyl isothiocyanate (AITC), a dietary phytochemical in some cruciferous vegetables, exhibits promising anticancer activities in many cancer models. However, previous data showed AITC to have a biphasic effect on cell viability, DNA damage and migration in human hepatoma HepG2 cells. Moreover, in a 3D co-culture of HUVEC with pericytes, it inhibited tube formation at high doses but promoted this at low doses, which confirmed its biphasic effect on angiogenesis. siRNA knockdown of Nrf2 and glutathione inhibition abolished the stimulation effect of AITC on cell migration and DNA damage. The biological activity of a novel AITC-conjugated silicon quantum dots (AITC-SiQDs) has been investigated for the first time. AITC-SiQDs showed similar anti-cancer properties to AITC at high doses while avoiding the low doses stimulation effect. In addition, AITC-SiQDs showed a lower and long-lasting activation of Nrf2 translocation into nucleus which correlated with their levels of cellular uptake, as detected by the intrinsic fluorescence of SiQDs. ROS production could be one of the mechanisms behind the anti-cancer effect of AITC-SiQDs. These data provide novel insights into the biphasic effect of AITC and highlight the application of nanotechnology to optimize the therapeutic potential of dietary isothiocyanates in cancer treatment.
KW - Antineoplastic Agents/chemistry
KW - Apoptosis/drug effects
KW - Cell Line, Tumor
KW - Cell Movement/drug effects
KW - Cell Proliferation/drug effects
KW - Cell Survival/drug effects
KW - Humans
KW - Isothiocyanates/chemistry
KW - NF-E2-Related Factor 2/metabolism
KW - Neovascularization, Physiologic/drug effects
KW - Quantum Dots/chemistry
KW - Reactive Oxygen Species/metabolism
KW - Silicon/chemistry
U2 - 10.1038/s41598-018-19353-7
DO - 10.1038/s41598-018-19353-7
M3 - Article
VL - 8
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
M1 - 1084
ER -