Anti-Candida targets and cytotoxicity of casuarinin isolated from Plinia cauliflora leaves in a bioactivity-guided study

Tatiana M Souza-Moreira, Juliana A Severi, Keunsook Lee, Kanya Preechasuth, Emerson Santos, Neil A R Gow, Carol A Munro, Wagner Vilegas, Rosemeire C L R Pietro

Research output: Contribution to journalArticle

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Abstract

In addition to the bio-guided investigation of the antifungal activity of Plinia cauliflora leaves against different Candida species, the major aim of the present study was the search for targets on the fungal cell. The most active antifungal fraction was purified by chromatography and characterized by NMR and mass spectrometry. The antifungal activity was evaluated against five Candida strains according to referenced guidelines. Cytotoxicity against fibroblast cells was determined. The likely targets of Candida albicans cells were assessed through interactions with ergosterol and cell wall composition, porosity and architecture. The chemical major component within the most active antifungal fraction of P. cauliflora leaves identified was the hydrolysable tannin casuarinin. The cytotoxic concentration was higher than the antifungal one. The first indication of plant target on cellular integrity was suggested by the antifungal activity ameliorated when using an osmotic support. The most important target for the tannin fraction studied was suggested by ultrastructural analysis of yeast cell walls revealing a denser mannan outer layer and wall porosity reduced. It is possible to imply that P. cauliflora targeted the C. albicans cell wall inducing some changes in the architecture, notably the outer glycoprotein layer, affecting the cell wall porosity without alteration of the polysaccharide or protein level.

Original languageEnglish
Pages (from-to)8095-8108
Number of pages14
JournalMolecules
Volume18
Issue number7
DOIs
Publication statusPublished - 2013

Fingerprint

Candida
Cytotoxicity
Bioactivity
Cell Wall
leaves
Porosity
Cells
Candida albicans
porosity
Hydrolyzable Tannins
Mannans
Ergosterol
Tannins
polysaccharides
yeast
Polysaccharides
fibroblasts
Chromatography
chromatography
Fibroblasts

Keywords

  • Antifungal Agents
  • Candida
  • Candidiasis
  • Cell Wall
  • Glycoproteins
  • Hydrolyzable Tannins
  • Microbial Sensitivity Tests
  • Myrtaceae
  • Plant Leaves
  • Porosity

Cite this

Souza-Moreira, T. M., Severi, J. A., Lee, K., Preechasuth, K., Santos, E., Gow, N. A. R., ... Pietro, R. C. L. R. (2013). Anti-Candida targets and cytotoxicity of casuarinin isolated from Plinia cauliflora leaves in a bioactivity-guided study. Molecules, 18(7), 8095-8108. https://doi.org/10.3390/molecules18078095

Anti-Candida targets and cytotoxicity of casuarinin isolated from Plinia cauliflora leaves in a bioactivity-guided study. / Souza-Moreira, Tatiana M; Severi, Juliana A; Lee, Keunsook; Preechasuth, Kanya; Santos, Emerson; Gow, Neil A R; Munro, Carol A; Vilegas, Wagner; Pietro, Rosemeire C L R.

In: Molecules, Vol. 18, No. 7, 2013, p. 8095-8108.

Research output: Contribution to journalArticle

Souza-Moreira, TM, Severi, JA, Lee, K, Preechasuth, K, Santos, E, Gow, NAR, Munro, CA, Vilegas, W & Pietro, RCLR 2013, 'Anti-Candida targets and cytotoxicity of casuarinin isolated from Plinia cauliflora leaves in a bioactivity-guided study', Molecules, vol. 18, no. 7, pp. 8095-8108. https://doi.org/10.3390/molecules18078095
Souza-Moreira, Tatiana M ; Severi, Juliana A ; Lee, Keunsook ; Preechasuth, Kanya ; Santos, Emerson ; Gow, Neil A R ; Munro, Carol A ; Vilegas, Wagner ; Pietro, Rosemeire C L R. / Anti-Candida targets and cytotoxicity of casuarinin isolated from Plinia cauliflora leaves in a bioactivity-guided study. In: Molecules. 2013 ; Vol. 18, No. 7. pp. 8095-8108.
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