Antifungal defense of probiotic Lactobacillus rhamnosus GG is mediated by blocking adhesion and nutrient depletion

Daniela Mailaender-Sanchez, Christina Braunsdorf, Christian Grumaz, Christoph Mueller, Stefan Lorenz, Philip Stevens, Jeanette Wagener, Betty Hebecker, Bernhard Hube, Franz Bracher, Kai Sohn, Martin Schaller*

*Corresponding author for this work

Research output: Contribution to journalArticle

10 Citations (Scopus)
7 Downloads (Pure)

Abstract

Candida albicans is an inhabitant of mucosal surfaces in healthy individuals but also the most common cause of fungal nosocomial blood stream infections, associated with high morbidity and mortality. As such life-threatening infections often disseminate from superficial mucosal infections we aimed to study the use of probiotic Lactobacillus rhamnosus GG (LGG) in prevention of mucosal C. albicans infections. Here, we demonstrate that LGG protects oral epithelial tissue from damage caused by C. albicans in our in vitro model of oral candidiasis. Furthermore, we provide insights into the mechanisms behind this protection and dissect direct and indirect effects of LGG on C. albicans pathogenicity. C. albicans viability was not affected by LGG. Instead, transcriptional profiling using RNA-Seq indicated dramatic metabolic reprogramming of C. albicans. Additionally, LGG had a significant impact on major virulence attributes, including adhesion, invasion, and hyphal extension, whose reduction, consequently, prevented epithelial damage. This was accompanied by glucose depletion and repression of ergosterol synthesis, caused by LGG, but also due to blocked adhesion sites. Therefore, LGG protects oral epithelia against C. albicans infection by preventing fungal adhesion, invasion and damage, driven, at least in parts, by metabolic reprogramming due to nutrient limitation caused by LGG.

Original languageEnglish
Article number0184438
Number of pages19
JournalPloS ONE
Volume12
Issue number10
DOIs
Publication statusPublished - 12 Oct 2017

Keywords

  • VULVO-VAGINAL CANDIDIASIS
  • IRRITABLE-BOWEL-SYNDROME
  • FUNGAL-INFECTIONS
  • RANDOMIZED-TRIAL
  • GENE-EXPRESSION
  • FATTY-ACID
  • ALBICANS
  • DIARRHEA
  • ASSAY
  • SUPPLEMENTATION

Cite this

Mailaender-Sanchez, D., Braunsdorf, C., Grumaz, C., Mueller, C., Lorenz, S., Stevens, P., ... Schaller, M. (2017). Antifungal defense of probiotic Lactobacillus rhamnosus GG is mediated by blocking adhesion and nutrient depletion. PloS ONE, 12(10), [0184438]. https://doi.org/10.1371/journal.pone.0184438

Antifungal defense of probiotic Lactobacillus rhamnosus GG is mediated by blocking adhesion and nutrient depletion. / Mailaender-Sanchez, Daniela; Braunsdorf, Christina; Grumaz, Christian; Mueller, Christoph; Lorenz, Stefan; Stevens, Philip; Wagener, Jeanette; Hebecker, Betty; Hube, Bernhard; Bracher, Franz; Sohn, Kai; Schaller, Martin.

In: PloS ONE, Vol. 12, No. 10, 0184438, 12.10.2017.

Research output: Contribution to journalArticle

Mailaender-Sanchez, D, Braunsdorf, C, Grumaz, C, Mueller, C, Lorenz, S, Stevens, P, Wagener, J, Hebecker, B, Hube, B, Bracher, F, Sohn, K & Schaller, M 2017, 'Antifungal defense of probiotic Lactobacillus rhamnosus GG is mediated by blocking adhesion and nutrient depletion', PloS ONE, vol. 12, no. 10, 0184438. https://doi.org/10.1371/journal.pone.0184438
Mailaender-Sanchez D, Braunsdorf C, Grumaz C, Mueller C, Lorenz S, Stevens P et al. Antifungal defense of probiotic Lactobacillus rhamnosus GG is mediated by blocking adhesion and nutrient depletion. PloS ONE. 2017 Oct 12;12(10). 0184438. https://doi.org/10.1371/journal.pone.0184438
Mailaender-Sanchez, Daniela ; Braunsdorf, Christina ; Grumaz, Christian ; Mueller, Christoph ; Lorenz, Stefan ; Stevens, Philip ; Wagener, Jeanette ; Hebecker, Betty ; Hube, Bernhard ; Bracher, Franz ; Sohn, Kai ; Schaller, Martin. / Antifungal defense of probiotic Lactobacillus rhamnosus GG is mediated by blocking adhesion and nutrient depletion. In: PloS ONE. 2017 ; Vol. 12, No. 10.
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abstract = "Candida albicans is an inhabitant of mucosal surfaces in healthy individuals but also the most common cause of fungal nosocomial blood stream infections, associated with high morbidity and mortality. As such life-threatening infections often disseminate from superficial mucosal infections we aimed to study the use of probiotic Lactobacillus rhamnosus GG (LGG) in prevention of mucosal C. albicans infections. Here, we demonstrate that LGG protects oral epithelial tissue from damage caused by C. albicans in our in vitro model of oral candidiasis. Furthermore, we provide insights into the mechanisms behind this protection and dissect direct and indirect effects of LGG on C. albicans pathogenicity. C. albicans viability was not affected by LGG. Instead, transcriptional profiling using RNA-Seq indicated dramatic metabolic reprogramming of C. albicans. Additionally, LGG had a significant impact on major virulence attributes, including adhesion, invasion, and hyphal extension, whose reduction, consequently, prevented epithelial damage. This was accompanied by glucose depletion and repression of ergosterol synthesis, caused by LGG, but also due to blocked adhesion sites. Therefore, LGG protects oral epithelia against C. albicans infection by preventing fungal adhesion, invasion and damage, driven, at least in parts, by metabolic reprogramming due to nutrient limitation caused by LGG.",
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AU - Mailaender-Sanchez, Daniela

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AU - Grumaz, Christian

AU - Mueller, Christoph

AU - Lorenz, Stefan

AU - Stevens, Philip

AU - Wagener, Jeanette

AU - Hebecker, Betty

AU - Hube, Bernhard

AU - Bracher, Franz

AU - Sohn, Kai

AU - Schaller, Martin

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KW - FUNGAL-INFECTIONS

KW - RANDOMIZED-TRIAL

KW - GENE-EXPRESSION

KW - FATTY-ACID

KW - ALBICANS

KW - DIARRHEA

KW - ASSAY

KW - SUPPLEMENTATION

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