Antifungal rhizosphere bacteria can increase as response to the presence of saprotrophic fungi

Wietse De Boer, Maria P J Hundscheid, Paulien J A Klein Gunnewiek, Annelies S. De Ridder-Duine, Cecile Thion, Johannes A. Van Veen, Annemieke Van Der Wal

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Abstract

Knowledge on the factors that determine the composition of bacterial communities in the vicinity of roots (rhizosphere) is essential to understand plant-soil interactions. Plant species identity, plant growth stage and soil properties have been indicated as major determinants of rhizosphere bacterial community composition. Here we show that the presence of saprotrophic fungi can be an additional factor steering rhizosphere bacterial community composition and functioning. We studied the impact of presence of two common fungal rhizosphere inhabitants (Mucor hiemalis and Trichoderma harzianum) on the composition of cultivable bacterial communities developing in the rhizosphere of Carex arenaria (sand sedge) in sand microcosms. Identification and phenotypic characterization of bacterial isolates revealed clear shifts in the rhizosphere bacterial community composition by the presence of two fungal strains (M. hiemalis BHB1 and T. harzianum PvdG2), whereas another M. hiemalis strain did not show this effect. Presence of both M. hiemalis BHB1 and T. harzianum PvdG2 resulted in a significant increase of chitinolytic and (in vitro) antifungal bacteria. The latter was most pronounced for M. hiemalis BHB1, an isolate from Carex roots, which stimulated the development of the bacterial genera Achromobacter and Stenotrophomonas. In vitro tests showed that these genera were strongly antagonistic against M. hiemalis but also against the plant-pathogenic fungus Rhizoctonia solani. The most likely explanation for fungal-induced shifts in the composition of rhizosphere bacteria is that bacteria are being selected which are successful in competing with fungi for root exudates. Based on the results we propose that measures increasing saprotrophic fungi in agricultural soils should be explored as an alternative approach to enhance natural biocontrol against soil-borne plant-pathogenic fungi, namely by stimulating indigenous antifungal rhizosphere bacteria.

Original languageEnglish
Article numbere0137988
Number of pages15
JournalPloS ONE
Volume10
Issue number9
Early online date22 Sep 2015
DOIs
Publication statusPublished - 22 Sep 2015

Fingerprint

Mucor hiemalis
Rhizosphere
rhizosphere bacteria
Fungi
rhizosphere
Bacteria
bacterial communities
fungi
Trichoderma harzianum
Chemical analysis
Soils
Soil
Carex
plant pathogenic fungi
Sand
Biocontrol
Achromobacter
Stenotrophomonas
sand
soil-plant interactions

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

De Boer, W., Hundscheid, M. P. J., Gunnewiek, P. J. A. K., De Ridder-Duine, A. S., Thion, C., Van Veen, J. A., & Van Der Wal, A. (2015). Antifungal rhizosphere bacteria can increase as response to the presence of saprotrophic fungi. PloS ONE, 10(9), [e0137988]. https://doi.org/10.1371/journal.pone.0137988

Antifungal rhizosphere bacteria can increase as response to the presence of saprotrophic fungi. / De Boer, Wietse; Hundscheid, Maria P J; Gunnewiek, Paulien J A Klein; De Ridder-Duine, Annelies S.; Thion, Cecile; Van Veen, Johannes A.; Van Der Wal, Annemieke.

In: PloS ONE, Vol. 10, No. 9, e0137988, 22.09.2015.

Research output: Contribution to journalArticle

De Boer, W, Hundscheid, MPJ, Gunnewiek, PJAK, De Ridder-Duine, AS, Thion, C, Van Veen, JA & Van Der Wal, A 2015, 'Antifungal rhizosphere bacteria can increase as response to the presence of saprotrophic fungi', PloS ONE, vol. 10, no. 9, e0137988. https://doi.org/10.1371/journal.pone.0137988
De Boer W, Hundscheid MPJ, Gunnewiek PJAK, De Ridder-Duine AS, Thion C, Van Veen JA et al. Antifungal rhizosphere bacteria can increase as response to the presence of saprotrophic fungi. PloS ONE. 2015 Sep 22;10(9). e0137988. https://doi.org/10.1371/journal.pone.0137988
De Boer, Wietse ; Hundscheid, Maria P J ; Gunnewiek, Paulien J A Klein ; De Ridder-Duine, Annelies S. ; Thion, Cecile ; Van Veen, Johannes A. ; Van Der Wal, Annemieke. / Antifungal rhizosphere bacteria can increase as response to the presence of saprotrophic fungi. In: PloS ONE. 2015 ; Vol. 10, No. 9.
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