Antigen presentation by macrophages is enhanced by the uptake of necrotic, but not apoptotic, cells

Robert Norman Barker, Lars Peter Erwig, K. S. Hill, A. Devine, W. P. Pearce, Andrew Jackson Rees

Research output: Contribution to journalArticle

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Abstract

The aim of this study was to determine whether phagocytosis of necrotic or apoptotic cells affects antigen presentation by murine bone marrow-derived macrophages. After uptake of necrotic neutrophils, macrophages were able to stimulate significantly higher T cell proliferation in vitro against both the recall antigen albumin and the mitogen concanavalin A. No such effect was seen following phagocytosis of apoptotic neutrophils. Flow cytometry revealed that, within 4h of ingestion, macrophages that had taken up the necrotic cells expressed higher levels of CD40 than those that had phagocytosed apoptotic cells. Macrophage cultures pulsed with apoptotic, but not necrotic, neutrophils contained higher levels of transforming growth factor beta1, but lower concentrations of tumour necrosis factor alpha, compared to untreated controls. Our interpretation of these results is that macrophages that have taken up necrotic neutrophils co-stimulate T cells with greater efficiency due to rapid CD40 up-regulation, whereas those that have ingested apoptotic cells are not only ineffective in co-stimulation, but also secrete inhibitory cytokine.

Original languageEnglish
Pages (from-to)220-225
Number of pages5
JournalClinical and Experimental Immunology
Volume127
Issue number2
DOIs
Publication statusPublished - Feb 2002

Keywords

  • antigen presentation
  • apoptosis
  • co-stimulatory molecules
  • macrophages
  • transforming growth factor beta
  • ACTIVATED MACROPHAGE
  • INNATE IMMUNITY
  • TGF-BETA
  • NEUTROPHILS
  • CYTOKINES
  • PHAGOCYTOSIS
  • TOLERANCE

Cite this

Antigen presentation by macrophages is enhanced by the uptake of necrotic, but not apoptotic, cells. / Barker, Robert Norman; Erwig, Lars Peter; Hill, K. S.; Devine, A.; Pearce, W. P.; Rees, Andrew Jackson.

In: Clinical and Experimental Immunology, Vol. 127, No. 2, 02.2002, p. 220-225.

Research output: Contribution to journalArticle

Barker, Robert Norman ; Erwig, Lars Peter ; Hill, K. S. ; Devine, A. ; Pearce, W. P. ; Rees, Andrew Jackson. / Antigen presentation by macrophages is enhanced by the uptake of necrotic, but not apoptotic, cells. In: Clinical and Experimental Immunology. 2002 ; Vol. 127, No. 2. pp. 220-225.
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