Antigenic comparison of a truncated form of VP2 of infectious pancreatic necrosis (IPN) virus expressed in four different cell types

Marie Labus, Suzanne Breeman, D. A. Smail, A. E. Ellis, M. Kervick, William Thomas Melvin

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9 Citations (Scopus)

Abstract

A truncated form of the structural protein VP2 (truncVP2) of infectious pancreatic necrosis (IPN) virus encompassing amino acids 147-307 was expressed in bacterial, yeast, piscine and mammalian cells. All four recombinant antigens were recognised by a VP2-specific monoclonal antibody by ELISA and immunoblot analysis. However, the minimum amount of r-truncVP2 needed for detection by these methods varies depending on the cell type used for expression. Furthermore, all four recombinant preparations, when used to immunise Atlantic salmon, were capable of inducing antibodies reactive with whole IPNV in ELISA. (C) 2001 Academic Press.

Original languageEnglish
Pages (from-to)203-216
Number of pages13
JournalFish & Shellfish Immunology
Volume11
Issue number3
DOIs
Publication statusPublished - 2001

Keywords

  • infectious pancreatic necrosis virus
  • monoclonal antibody
  • recombinant protein
  • ELISA
  • immunoblot
  • SEROTYPE-SPECIFIC EPITOPE
  • HIGH-LEVEL EXPRESSION
  • CAPSID PROTEIN VP2
  • SALMO-SALAR L
  • MONOCLONAL-ANTIBODIES
  • ATLANTIC SALMON
  • PICHIA-PASTORIS
  • RECOMBINANT VP2
  • IDENTIFICATION
  • GLYCOSYLATION

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