Antimicrobial activities of marine fungi from Malaysia

N. Zainuddin, S.A. Alias, C.W. Lee, R. Ebel, N.A. Othman, M.R. Mukhtar, K. Awang

Research output: Contribution to journalArticle

10 Citations (Scopus)
12 Downloads (Pure)

Abstract

In a systematic screening effort, extracts of marine fungi from Malaysia were investigated for antimicrobial activity and potentially active secondary metabolites. In preliminary experiments, the plug assay method was employed to screen 152 strains for antimicrobial activity. Of these, 82 exhibited biological activity, with most of the active strains (90.2%) having antibacterial activity, mainly towards Gram-positive bacteria, while only 9.6% had antifungal activity. On the basis of these results, five marine fungi, Fasciatispora nypae, Caryosporella rhizophorae, Melaspilea mangrovei, Leptosphaeria sp. and ascomycete strain 19 (NF) were selected for further investigation to confirm their biological activity by the disc diffusion assay method. The selected species had various degrees of activity against the test microorganisms, depending on culture conditions (stationary vs. shaking cultures) and incubation time (10-25 days). Only F. nypae showed a wider range of antifungal and antibacterial activity as compared to the remaining fungal strains under investigation. Therefore, bioactivity-guided fractionation was undertaken to isolate the active principles, resulting in the characterisation of 2,2,7-trimethyl-2H-chromen-5-ol (1) which had antimicrobial activity towards test microorganisms. The structure of 1, which previously had only been reported as a synthetic intermediate, but not as a natural product, was elucidated by mass spectrometry in conjunction with one- and two-dimensional NMR spectroscopy.
Original languageEnglish
Pages (from-to)507-513
Number of pages7
JournalBotanica Marina
Volume53
Issue number6
DOIs
Publication statusPublished - Dec 2010

Fingerprint

marine fungi
antimicrobial activity
Malaysia
anti-infective agents
fungus
microorganism
assay
Melaspilea
bioactive properties
bioactivity
secondary metabolite
Leptosphaeria
microorganisms
nuclear magnetic resonance
assays
Gram-positive bacteria
fractionation
mass spectrometry
incubation
spectroscopy

Keywords

  • antimicrobial activity
  • biologically active natural products
  • Fasciatispora nypae
  • marine fungi

Cite this

Zainuddin, N., Alias, S. A., Lee, C. W., Ebel, R., Othman, N. A., Mukhtar, M. R., & Awang, K. (2010). Antimicrobial activities of marine fungi from Malaysia. Botanica Marina, 53(6), 507-513. https://doi.org/10.1515/BOT.2010.067

Antimicrobial activities of marine fungi from Malaysia. / Zainuddin, N.; Alias, S.A.; Lee, C.W.; Ebel, R.; Othman, N.A.; Mukhtar, M.R.; Awang, K.

In: Botanica Marina, Vol. 53, No. 6, 12.2010, p. 507-513.

Research output: Contribution to journalArticle

Zainuddin, N, Alias, SA, Lee, CW, Ebel, R, Othman, NA, Mukhtar, MR & Awang, K 2010, 'Antimicrobial activities of marine fungi from Malaysia', Botanica Marina, vol. 53, no. 6, pp. 507-513. https://doi.org/10.1515/BOT.2010.067
Zainuddin N, Alias SA, Lee CW, Ebel R, Othman NA, Mukhtar MR et al. Antimicrobial activities of marine fungi from Malaysia. Botanica Marina. 2010 Dec;53(6):507-513. https://doi.org/10.1515/BOT.2010.067
Zainuddin, N. ; Alias, S.A. ; Lee, C.W. ; Ebel, R. ; Othman, N.A. ; Mukhtar, M.R. ; Awang, K. / Antimicrobial activities of marine fungi from Malaysia. In: Botanica Marina. 2010 ; Vol. 53, No. 6. pp. 507-513.
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