Antimony speciation in soils: Improving the detection limits using post-column pre-reduction hydride generation atomic fluorescence spectroscopy (HPLC/pre-reduction/HG-AFS)

Waldo Quiroz, David Olivares, Manuel Bravo, Jorg Feldmann, Andrea Raab

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

HG-AFS is highly sensitive and low cost detection system and its use for antimony chemical speciation coupled to HPLC is gaining popularity. However speciation analysis in soils is strongly hampered because the most efficient extractant reported in the literature (oxalic acid) strongly inhibits the generation of SbH3 by Sb(V), the major species in this kind of matrix, severely affecting its detection limits. The purpose of this research is to reduce the detection limit of Sb(V), by using a post column on-line reduction system with L-cysteine reagent (HPLC/pre-reduction/HG-AFS). The system was optimized by experimental design, optimum conditions found were 2% (w/v) and 10 degrees C temperature coil. Detection limits of Sb(V) and Sb(III) in oxalic acid (0.25 mol L-1) were improved from 0.3 and 0.1 mu g L-1 to 0.07 and 0.07 mu g L-1, respectively. The methodology developed was applied to Chilean soils, where Sb(V) was the predominant species. (C) 2011 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)593-598
Number of pages6
JournalTalanta
Volume84
Issue number2
Early online date15 Jan 2011
DOIs
Publication statusPublished - 15 Apr 2011

Keywords

  • Antimony speciation
  • Soils
  • HC-AFS
  • plasma-mass spectrometry
  • ID-ICP-MS
  • ion chromatography
  • flow-injection
  • HG-AFS
  • absorption spectrometry
  • environmental-samples
  • emission-spectrometry
  • assisted extraction
  • biological samples

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