Antioxidant capacity is repeatable across years but does not consistently correlate with a marker of peroxidation in a free-living passerine bird

Charlotte Récapet (Corresponding Author), Mathilde Arrive, Blandine Doligez, Pierre Bize

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Oxidative stress occurs when reactive oxygen species (ROS) exceed antioxidant defences, which can have deleterious effects on cell function, health and survival. Therefore, organisms are expected to finely regulate pro-oxidant and antioxidant processes. ROS are mainly produced through aerobic metabolism and vary in response to changes in energetic requirements, whereas antioxidants may be enhanced, depleted or show no changes in response to changes in ROS levels. We investigated the repeatability, within-individual variation and correlation across different conditions of two plasmatic markers of the oxidative balance in 1108 samples from 635 free-living adult collared flycatchers (Ficedula albicollis). We sought to manipulate energy constraints by increasing wing load in 2012 and 2013 and by providing additional food in 2014. We then tested the relative importance of within- and between-individual variation on reactive oxygen metabolites (ROMs), a marker of lipid and protein peroxidation, and on non-enzymatic antioxidant defences (OXY test). We also investigated whether the experimental treatments modified the correlation between markers. Antioxidant defences were repeatable (range of repeatability estimates = 0.128 - 0.581), whereas ROMs were not (0 - 0.061). Antioxidants varied neither between incubation and nestling feeding nor between sexes. ROMs increased from incubation to nestling feeding in females and were higher in females than males. Antioxidant defences and ROM concentration were globally positively correlated, but the correlation varied between experimental conditions and between years. Hence, the management of oxidative balance in wild animals appears flexible under variable environmental conditions, an observation which should be confirmed over a wider range of markers.
Original languageEnglish
Pages (from-to)283-298
Number of pages16
JournalJournal of Comparative Physiology B
Volume189
Issue number2
Early online date8 Mar 2019
DOIs
Publication statusPublished - Apr 2019

Fingerprint

peroxidation
Birds
passerine
repeatability
antioxidant
reactive oxygen species
Antioxidants
bird
antioxidants
Metabolites
birds
Reactive Oxygen Species
metabolite
antioxidant activity
Oxygen
oxygen
individual variation
nestling
incubation
Songbirds

Keywords

  • energetic constraints
  • food supplementation
  • reactive oxygen metabolites
  • antioxidant defences
  • oxidative stress
  • Ficedula albicollis
  • Oxidative stress
  • Food supplementation
  • Energetic constraints
  • Antioxidant defences
  • Reactive oxygen metabolites

Cite this

Antioxidant capacity is repeatable across years but does not consistently correlate with a marker of peroxidation in a free-living passerine bird. / Récapet, Charlotte (Corresponding Author); Arrive, Mathilde; Doligez, Blandine; Bize, Pierre.

In: Journal of Comparative Physiology B, Vol. 189, No. 2, 04.2019, p. 283-298.

Research output: Contribution to journalArticle

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title = "Antioxidant capacity is repeatable across years but does not consistently correlate with a marker of peroxidation in a free-living passerine bird",
abstract = "Oxidative stress occurs when reactive oxygen species (ROS) exceed antioxidant defences, which can have deleterious effects on cell function, health and survival. Therefore, organisms are expected to finely regulate pro-oxidant and antioxidant processes. ROS are mainly produced through aerobic metabolism and vary in response to changes in energetic requirements, whereas antioxidants may be enhanced, depleted or show no changes in response to changes in ROS levels. We investigated the repeatability, within-individual variation and correlation across different conditions of two plasmatic markers of the oxidative balance in 1108 samples from 635 free-living adult collared flycatchers (Ficedula albicollis). We sought to manipulate energy constraints by increasing wing load in 2012 and 2013 and by providing additional food in 2014. We then tested the relative importance of within- and between-individual variation on reactive oxygen metabolites (ROMs), a marker of lipid and protein peroxidation, and on non-enzymatic antioxidant defences (OXY test). We also investigated whether the experimental treatments modified the correlation between markers. Antioxidant defences were repeatable (range of repeatability estimates = 0.128 - 0.581), whereas ROMs were not (0 - 0.061). Antioxidants varied neither between incubation and nestling feeding nor between sexes. ROMs increased from incubation to nestling feeding in females and were higher in females than males. Antioxidant defences and ROM concentration were globally positively correlated, but the correlation varied between experimental conditions and between years. Hence, the management of oxidative balance in wild animals appears flexible under variable environmental conditions, an observation which should be confirmed over a wider range of markers.",
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author = "Charlotte R{\'e}capet and Mathilde Arrive and Blandine Doligez and Pierre Bize",
note = "Many thanks to the landowners of Gotland for access to the study sites; to Lars Gustafsson for logistics on the field; to fifteen students and field assistants for their contribution to fieldwork; to Fran{\cc}ois Criscuolo for his help designing the study and his comments on this manuscript; to Holger Schielzeth and Pierre de Villemereuil for their advice on adjusting repeatability and correlation estimates for measurement error and to Alan A. Cohen, David Costantini and anonymous reviewers for their comments on previous versions of this manuscript. This work was supported by the French National Center for Scientific Research (PICS France34 Switzerland to B.D.); the French Ministry of Research (PhD fellowship to C.R.); the University of Aberdeen (stipend to C.R.); the L’Or{\'e}al Foundation-UNESCO “For Women in Science” program (fellowship to C.R.); the R{\'e}gion Rh{\^o}ne-Alpes (Explora’doc mobility grant to C.R.); the F{\'e}d{\'e}ration de Recherche 41 BioEnvironnement et Sant{\'e} (training grant to CR); the Rectors’ Conference of the Swiss Universities (joint doctoral program grant to C.R.) and the Fondation pour l'Universit{\'e} de Lausanne (exchange grant to C.R.); and the Journal of Experimental Biology (travel grant to CR).",
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N1 - Many thanks to the landowners of Gotland for access to the study sites; to Lars Gustafsson for logistics on the field; to fifteen students and field assistants for their contribution to fieldwork; to François Criscuolo for his help designing the study and his comments on this manuscript; to Holger Schielzeth and Pierre de Villemereuil for their advice on adjusting repeatability and correlation estimates for measurement error and to Alan A. Cohen, David Costantini and anonymous reviewers for their comments on previous versions of this manuscript. This work was supported by the French National Center for Scientific Research (PICS France34 Switzerland to B.D.); the French Ministry of Research (PhD fellowship to C.R.); the University of Aberdeen (stipend to C.R.); the L’Oréal Foundation-UNESCO “For Women in Science” program (fellowship to C.R.); the Région Rhône-Alpes (Explora’doc mobility grant to C.R.); the Fédération de Recherche 41 BioEnvironnement et Santé (training grant to CR); the Rectors’ Conference of the Swiss Universities (joint doctoral program grant to C.R.) and the Fondation pour l'Université de Lausanne (exchange grant to C.R.); and the Journal of Experimental Biology (travel grant to CR).

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